BMRB Entry 7351

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7351
MolProbity Validation Chart

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Title:
NMR STRUCTURE OF HUMAN RTF1 PLUS3 DOMAIN.
Deposition date:
2006-12-01
Original release date:
2007-10-23
Authors:
Truffault, V.; Diercks, T.; Ab, E.; De Jong, R.; Daniels, M.; Kaptein, R.; Folkers, G.
Citation:

Citation: De Jong, R.; Truffault, V.; Diercks, T.; Ab, E.; Daniels, M.; Kaptein, R.; Folkers, G.. "Structure and DNA binding of the human Rtf1 Plus3 domain."  Structure 16, 149-159 (2008).
PubMed: 18184592

Assembly members:

Assembly members:
KIAA0252_PROTEIN, polymer, 153 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: HUMAN   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: ESCHERICHIA COLI   Vector: PET15B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KIAA0252_PROTEIN: MGSSHHHHHHSSGLVPRGSH MVSLPEELNRVRLSRHKLER WCHMPFFAKTVTGCFVRIGI GNHNSKPVYRVAEITGVVET AKVYQLGGTRTNKGLQLRHG NDQRVFRLEFVSNQEFTESE FMKWKEAMFSAGMQLPTLDE INKKELSIKEALN

Data sets:
Data typeCount
13C chemical shifts631
15N chemical shifts157
1H chemical shifts1040

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KIAA0252_PROTEIN1

Entities:

Entity 1, KIAA0252_PROTEIN 153 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METVALSERLEUPROGLUGLULEUASNARG
4   VALARGLEUSERARGHISLYSLEUGLUARG
5   TRPCYSHISMETPROPHEPHEALALYSTHR
6   VALTHRGLYCYSPHEVALARGILEGLYILE
7   GLYASNHISASNSERLYSPROVALTYRARG
8   VALALAGLUILETHRGLYVALVALGLUTHR
9   ALALYSVALTYRGLNLEUGLYGLYTHRARG
10   THRASNLYSGLYLEUGLNLEUARGHISGLY
11   ASNASPGLNARGVALPHEARGLEUGLUPHE
12   VALSERASNGLNGLUPHETHRGLUSERGLU
13   PHEMETLYSTRPLYSGLUALAMETPHESER
14   ALAGLYMETGLNLEUPROTHRLEUASPGLU
15   ILEASNLYSLYSGLULEUSERILELYSGLU
16   ALALEUASN

Samples:

sample: KIAA0252_PROTEIN, [U-13C; U-15N], mM; NaCl 150 mM; Na_2 HPO_4 50 mM; NaH_2 PO_4 50 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 200 mM; pH: 6.0; pressure: 1.0 ATM; temperature: 298.0 K

Experiments:

NameSampleSample stateSample conditions
HH-NOESYsampleisotropicsample_conditions_1
HNCACOsampleisotropicsample_conditions_1
HNCACBsampleisotropicsample_conditions_1
CBCACONHsampleisotropicsample_conditions_1
HNCAsampleisotropicsample_conditions_1
HBHACONHsampleisotropicsample_conditions_1
HNCAHAsampleisotropicsample_conditions_1
CCH-COSYsampleisotropicsample_conditions_1
HCCH-TOCSYsampleisotropicsample_conditions_1

Software:

CNS, A Brunger, P Adams, M Clore, P Gros, M Nilges and R Read - refinement

SPARKY v3.110, T Goddard - structure solution

CYANA2.1, P Guntert, C Mumenthaler and K Wuthrich - structure solution

NMR spectrometers:

  • BRUKER DMX 700 MHz

Related Database Links:

BMRB 11330
PDB
GB EAW92491
REF XP_004609643

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks