BMRB Entry 7326
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7326
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Placzek, William; Etezady-Esfarjani, Touraj; Herrmann, Torsten; Pedrini, Bill; Peti, Wolfgang; Alimenti, Claudio; Luporini, Pierangelo; Wuthrich, Kurt. "Cold-adapted signal proteins: NMR structures of pheromones from the Antarctic
ciliate Euplotes nobilii." IUBMB Life 59, 578-585 (2007).
PubMed: 17701553
Assembly members:
En-1, polymer, 52 residues, 5630.1 Da.
Natural source: Common Name: Euplotes nobilii Taxonomy ID: 184062 Superkingdom: Eukaryota Kingdom: Alveolata Genus/species: Euplotes nobilii
Experimental source: Production method: Purified from the natural source
Entity Sequences (FASTA):
En-1: NPEDWFTPDTCAYGDSNTAW
TTCTTPGQTCYTCCSSCFDV
VGEQACQMSAQC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 55 |
| 1H chemical shifts | 286 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | En-1 monomer | 1 |
Entities:
Entity 1, En-1 monomer 52 residues - 5630.1 Da.
| 1 | ASN | PRO | GLU | ASP | TRP | PHE | THR | PRO | ASP | THR | ||||
| 2 | CYS | ALA | TYR | GLY | ASP | SER | ASN | THR | ALA | TRP | ||||
| 3 | THR | THR | CYS | THR | THR | PRO | GLY | GLN | THR | CYS | ||||
| 4 | TYR | THR | CYS | CYS | SER | SER | CYS | PHE | ASP | VAL | ||||
| 5 | VAL | GLY | GLU | GLN | ALA | CYS | GLN | MET | SER | ALA | ||||
| 6 | GLN | CYS |
Samples:
sample_1: En-1, [U-13C], 0.9 ± 0.05 mM; Sodium Phosphate 20 ± 1 mM
conditions_1: ionic strength: 20 mM; pH: 6.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D TOCSY | sample_1 | not available | conditions_1 |
| 2D COSY | sample_1 | not available | conditions_1 |
| 2D natural abundance [13C, 1H]- HSQC | sample_1 | not available | conditions_1 |
| 2D NOESY | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 600 MHz
- Bruker Avance 900 MHz