BMRB Entry 7319
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7319
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Mueller, Geoffrey; DeRose, Eugene; Kirby, Thomas; London, Robert. "NMR assignment of polymerase beta labeled with 2H, 13C, and 15N in complex with substrate DNA" Biomol. NMR Assignments 1, 33-35 (2007).
PubMed: 19421423
Assembly members:
pol beta, polymer, 335 residues, 39000 Da.
double gap double hairpin dna, polymer, 22 residues, Formula weight is not available
Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
pol beta: MSKRKAPQETLNGGITDMLV
ELANFEKNVSQAIHKYNAYR
KAASVIAKYPHKIKSGAEAK
KLPGVGTKIAEKIDEFLATG
KLRKLEKIRQDDTSSSINFL
TRVTGIGPSAARKLVDEGIK
TLEDLRKNEDKLNHHQRIGL
KYFEDFEKRIPREEMLQMQD
IVLNEVKKLDPEYIATVCGS
FRRGAESSGDMDVLLTHPNF
TSESSKQPKLLHRVVEQLQK
VRFITDTLSKGETKFMGVCQ
LPSENDENEYPHRRIDIRLI
PKDQYYCGVLYFTGSDIFNK
NMRAHALEKGFTINEYTIRP
LGVTGVAGEPLPVDSEQDIF
DYIQWRYREPKDRSE
double gap double hairpin dna: CAGCGAAGCTGGTCGCGAAG
CG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 775 |
| 15N chemical shifts | 268 |
| 1H chemical shifts | 268 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | polyermase beta | 1 |
| 2 | double gap double hairpin dna | 2 |
Entities:
Entity 1, polyermase beta 335 residues - 39000 Da.
| 1 | MET | SER | LYS | ARG | LYS | ALA | PRO | GLN | GLU | THR | ||||
| 2 | LEU | ASN | GLY | GLY | ILE | THR | ASP | MET | LEU | VAL | ||||
| 3 | GLU | LEU | ALA | ASN | PHE | GLU | LYS | ASN | VAL | SER | ||||
| 4 | GLN | ALA | ILE | HIS | LYS | TYR | ASN | ALA | TYR | ARG | ||||
| 5 | LYS | ALA | ALA | SER | VAL | ILE | ALA | LYS | TYR | PRO | ||||
| 6 | HIS | LYS | ILE | LYS | SER | GLY | ALA | GLU | ALA | LYS | ||||
| 7 | LYS | LEU | PRO | GLY | VAL | GLY | THR | LYS | ILE | ALA | ||||
| 8 | GLU | LYS | ILE | ASP | GLU | PHE | LEU | ALA | THR | GLY | ||||
| 9 | LYS | LEU | ARG | LYS | LEU | GLU | LYS | ILE | ARG | GLN | ||||
| 10 | ASP | ASP | THR | SER | SER | SER | ILE | ASN | PHE | LEU | ||||
| 11 | THR | ARG | VAL | THR | GLY | ILE | GLY | PRO | SER | ALA | ||||
| 12 | ALA | ARG | LYS | LEU | VAL | ASP | GLU | GLY | ILE | LYS | ||||
| 13 | THR | LEU | GLU | ASP | LEU | ARG | LYS | ASN | GLU | ASP | ||||
| 14 | LYS | LEU | ASN | HIS | HIS | GLN | ARG | ILE | GLY | LEU | ||||
| 15 | LYS | TYR | PHE | GLU | ASP | PHE | GLU | LYS | ARG | ILE | ||||
| 16 | PRO | ARG | GLU | GLU | MET | LEU | GLN | MET | GLN | ASP | ||||
| 17 | ILE | VAL | LEU | ASN | GLU | VAL | LYS | LYS | LEU | ASP | ||||
| 18 | PRO | GLU | TYR | ILE | ALA | THR | VAL | CYS | GLY | SER | ||||
| 19 | PHE | ARG | ARG | GLY | ALA | GLU | SER | SER | GLY | ASP | ||||
| 20 | MET | ASP | VAL | LEU | LEU | THR | HIS | PRO | ASN | PHE | ||||
| 21 | THR | SER | GLU | SER | SER | LYS | GLN | PRO | LYS | LEU | ||||
| 22 | LEU | HIS | ARG | VAL | VAL | GLU | GLN | LEU | GLN | LYS | ||||
| 23 | VAL | ARG | PHE | ILE | THR | ASP | THR | LEU | SER | LYS | ||||
| 24 | GLY | GLU | THR | LYS | PHE | MET | GLY | VAL | CYS | GLN | ||||
| 25 | LEU | PRO | SER | GLU | ASN | ASP | GLU | ASN | GLU | TYR | ||||
| 26 | PRO | HIS | ARG | ARG | ILE | ASP | ILE | ARG | LEU | ILE | ||||
| 27 | PRO | LYS | ASP | GLN | TYR | TYR | CYS | GLY | VAL | LEU | ||||
| 28 | TYR | PHE | THR | GLY | SER | ASP | ILE | PHE | ASN | LYS | ||||
| 29 | ASN | MET | ARG | ALA | HIS | ALA | LEU | GLU | LYS | GLY | ||||
| 30 | PHE | THR | ILE | ASN | GLU | TYR | THR | ILE | ARG | PRO | ||||
| 31 | LEU | GLY | VAL | THR | GLY | VAL | ALA | GLY | GLU | PRO | ||||
| 32 | LEU | PRO | VAL | ASP | SER | GLU | GLN | ASP | ILE | PHE | ||||
| 33 | ASP | TYR | ILE | GLN | TRP | ARG | TYR | ARG | GLU | PRO | ||||
| 34 | LYS | ASP | ARG | SER | GLU |
Entity 2, double gap double hairpin dna 22 residues - Formula weight is not available
| 1 | DC | DA | DG | DC | DG | DA | DA | DG | DC | DT | ||||
| 2 | DG | DG | DT | DC | DG | DC | DG | DA | DA | DG | ||||
| 3 | DC | DG |
Samples:
sample_1: pol beta, [U-2H; U-13C; U-15N], 0.5 mM; dgdhp 0.5 mM; KCl 150 mM; sodium azide 3 mM; Tris buffer 150 mM
conditions_1: pH: 7.4; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D TROSY HNCO | sample_1 | not available | conditions_1 |
| 3D TROSY HN(CA)CO | sample_1 | not available | conditions_1 |
| 3D TROSY HN(CO)CA | sample_1 | not available | conditions_1 |
| 3D TROSY HNCA | sample_1 | not available | conditions_1 |
| 3D TROSY HNCACB | sample_1 | not available | conditions_1 |
| 3D TROSY HN(CO)CACB | sample_1 | not available | conditions_1 |
| 3D TROSY HN(COCA)CB | sample_1 | not available | conditions_1 |
| 15N separated NOE | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 800 MHz
Related Database Links:
| BMRB | 18267 5208 |
| PDB | |
| DBJ | BAC36630 BAE27405 BAE30399 |
| GB | AAA41900 AAA41901 AAB00389 AAH60998 AAH98668 |
| REF | NP_035260 NP_058837 XP_005066593 XP_005362476 |
| SP | P06766 Q8K409 |
| AlphaFold | P06766 Q8K409 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks