BMRB Entry 7318

Name: Backbone 1HN, 15N, and 13C Chemical Shift Assignments for wt Im7* (* denotes his-tag) and its variants, Im7*L53AI54A and Im7*YY
Published: 2007-05-04

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7318

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1HN, 15N, and 13C Chemical Shift Assignments for wt Im7* (* denotes his-tag) and its variants, Im7*L53AI54A and Im7*YY

Deposition date:

2006-10-17

Original release date:

2007-05-04

Authors:

Whittaker, Sara; Spence, Graham; Grossmann, Guenter; Radford, Sheena; Moore, Geoffrey

Citation:

Citation: Whittaker, Sara; Spence, Graham; Grossmann, Guenter; Radford, Sheena; Moore, Geoffrey. "NMR analysis of the conformational properties of the trapped on-pathway folding intermediate of the bacterial immunity protein Im7." J. Mol. Biol. 366, 1001-1015 (2007).
PubMed: 17188712

Assembly members:

Assembly members:
YY, polymer, 94 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Escherichia coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
YY: MEHHHHHHELKNSISDYTEA EFVQLLKEIEKENVAATDDV LDVLLEHFVKITEHPDGGGG GYYPSDNRDDSPEGIVKEIK EWRAANGKPGFKQG

Data sets:

assigned_chemical_shifts
- Im7*_YY_chemical_shifts

Data type	Count
13C chemical shifts	202
15N chemical shifts	68
1H chemical shifts	101

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Monomer	1

Entities:

Entity 1, Monomer 94 residues - Formula weight is not available

The sequence of YY contains an N-terminal His-tag. Residue numbering starts at residue 2 of wt Im7 (no his-tag). Compared with Im7*, the residues T51-I54 have been mutated to Gly.

1

MET

GLU

HIS

GLU

LEU

2

LYS

ASN

SER

ILE

SER

ASP

TYR

THR

GLU

ALA

3

GLU

PHE

VAL

GLN

LEU

LYS

GLU

ILE

GLU

4

LYS

GLU

ASN

VAL

ALA

THR

ASP

VAL

5

LEU

ASP

VAL

LEU

GLU

HIS

PHE

VAL

LYS

6

ILE

THR

GLU

HIS

PRO

ASP

GLY

7

GLY

TYR

PRO

SER

ASP

ASN

ARG

ASP

8

SER

PRO

GLU

GLY

ILE

VAL

LYS

GLU

ILE

LYS

9

GLU

TRP

ARG

ALA

ASN

GLY

LYS

PRO

GLY

10

PHE

LYS

GLN

GLY

Samples:

sample_1: Im7*, [U-13C; U-15N], 1 ± 0.2 mM; potassium phosphate buffer 50 mM; sodium sulphate 400 mM; H2O 90%; D2O 10%

sample_2: Im7*, [U-99% 15N], 1 ± 0.2 mM; potassium phosphate buffer 50 mM; sodium sulphate 400 mM; H2O 90%; D2O 10%

conditions_1: pH: 7; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N HSQC	not available	not available	conditions_1
HNCO	Samples_1_and_3_and_5	not available	conditions_1
HNCA	Samples_3_and_5	not available	conditions_1
HNCOCA	Samples_3_and_5_again	not available	conditions_1
CBCACONH	Samples_3_and_5_again2	not available	conditions_1
HNCACB	sample_1	not available	conditions_1
1H-1H-15N NOESY-HSQC	not available	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Varian Inova 500 MHz
Varian Inova 600 MHz
Varian Inova 750 MHz

GB	EYB47026
REF	WP_032277812