BMRB Entry 7312

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7312

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Title:
1H,13C and 15N resonance assignment of the VHS domain of human STAM1 protein
Deposition date:
2006-10-02
Original release date:
2009-10-20
Authors:
Lee, Bong-jin
Citation:

Citation: Hong, Yoon-Hun; Ahn, Hee-Chul; Lim, Jongsoo; Kim, Hong-Man; Ji, Hye-Young; Lee, Seunga; Kim, Ji-Hun; Park, Eun-Young; Song, Hyun-Kyu; Lee, Bong-Jin. "Identification of a novel ubiquitin binding site of STAM1 VHS domain by NMR spectroscopy"  FEBS Lett. 583, 287-292 (2009).
PubMed: 19111546

Assembly members:

Assembly members:
signal transducing adaptor molecule 1, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
signal transducing adaptor molecule 1: PLFATNPFDQDVEKATSEMN TAEDWGLILDICDKVGQSRT GPKDCLRSIMRRVNHKDPHV AMQALTLLGACVSNCGKIFH LEVCSRDFASEVSNVLNKGH PKVCEKLKALMVEWTDEFKN DPQLSLISAMIKNLKEQG

Data sets:
Data typeCount
13C chemical shifts453
15N chemical shifts130
1H chemical shifts489

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1VHS domain monomer1

Entities:

Entity 1, VHS domain monomer 138 residues - Formula weight is not available

1   PROLEUPHEALATHRASNPROPHEASPGLN
2   ASPVALGLULYSALATHRSERGLUMETASN
3   THRALAGLUASPTRPGLYLEUILELEUASP
4   ILECYSASPLYSVALGLYGLNSERARGTHR
5   GLYPROLYSASPCYSLEUARGSERILEMET
6   ARGARGVALASNHISLYSASPPROHISVAL
7   ALAMETGLNALALEUTHRLEULEUGLYALA
8   CYSVALSERASNCYSGLYLYSILEPHEHIS
9   LEUGLUVALCYSSERARGASPPHEALASER
10   GLUVALSERASNVALLEUASNLYSGLYHIS
11   PROLYSVALCYSGLULYSLEULYSALALEU
12   METVALGLUTRPTHRASPGLUPHELYSASN
13   ASPPROGLNLEUSERLEUILESERALAMET
14   ILELYSASNLEULYSGLUGLNGLY

Samples:

sample_1: signal transducing adaptor molecule 1, [U-99% 13C; U-98% 15N; U-70% 2H], 0.3 – 0.6 mM

conditions_1: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D [15N, 1H]-HSQCsample_1not availableconditions_1
2D [15N, 1H]-TROSYsample_1not availableconditions_1
3D TROSY-HNCAsample_1not availableconditions_1
3D TROSY-HN(CO)CAsample_1not availableconditions_1
3D TROSY-HNCACBsample_1not availableconditions_1
TROSY-HN(CO)CACBsample_1not availableconditions_1
3D TROSY-HN(CA)COsample_1not availableconditions_1
3D TROSY-HNCOsample_1not availableconditions_1
3D HBHA(CO)NHsample_1not availableconditions_1
3D 15N-edited NOESY-TROSYsample_1not availableconditions_1
3D 15N-edited TOCSY-TROSYsample_1not availableconditions_1
3D 13C-edited NOESY-HSQCsample_1not availableconditions_1
3D 13C-edited TOCSY-HSQCsample_1not availableconditions_1
3D HCCH-COSYsample_1not availableconditions_1
3D HCCH-TOCSYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker AVANCE 600 MHz
  • Bruker AVANCE 900 MHz

Related Database Links:

BMRB 16763 17059 17065
PDB
DBJ BAE26170 BAE28168 BAE34187 BAE37536 BAE40949
GB AAC50734 AAC52840 AAH30586 AAH44666 AAH55326
REF NP_001070310 NP_001102591 NP_003464 NP_035614 XP_001094352
SP P70297 Q92783
TPG DAA23567
AlphaFold P70297 Q92783

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks