BMRB Entry 7308

Title:
Backbone assignment and RDCs of L11 in complex with RNA and thiostrepton   PubMed: 17169991
Deposition date:
2006-09-28
Original release date:
2006-12-20
Authors:
Jonker, Henry; Ilin, Serge; Grimm, Steffen; Wohnert, Jens; Schwalbe, Harald
Citation:

Citation: Jonker, Henry; Ilin, Serge; Grimm, Steffen; Wohnert, Jens; Schwalbe, Harald. "L11 domain rearrangement upon binding to RNA and thiostrepton studied by NMR spectroscopy."  Nucleic Acids Res. 35, 441-454 (2007).

Assembly members:

Assembly members:
ribosomal L11 protein, polymer, 141 residues, 15088 Da.
23S GAR RNA, polymer, 60 residues, 19258 Da.
Thiostrepton antibiotic, polymer, 15 residues, 1665 Da.

Natural source:

Natural source:   Common Name: Thermotoga maritima   Taxonomy ID: 2336   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermotoga maritima

Experimental source:   Production method: recombinant technology

Experimental source:

Natural source:   Common Name: Thermotoga maritima   Taxonomy ID: 2336   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermotoga maritima

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts354
15N chemical shifts127
1H chemical shifts127
residual dipolar couplings120

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1L11 protein1
2GAR RNA2
3Thiostrepton3

Entities:

Entity 1, L11 protein 141 residues - 15088 Da.

1-141, Thermotoga maritima

1   METALALYSLYSVALALAALAGLNILELYS
2   LEUGLNLEUPROALAGLYLYSALATHRPRO
3   ALAPROPROVALGLYPROALALEUGLYGLN
4   HISGLYVALASNILEMETGLUPHECYSLYS
5   ARGPHEASNALAGLUTHRALAASPLYSALA
6   GLYMETILELEUPROVALVALILETHRVAL
7   TYRGLUASPLYSSERPHETHRPHEILEILE
8   LYSTHRPROPROALASERPHELEULEULYS
9   LYSALAALAGLYILEGLULYSGLYSERSER
10   GLUPROLYSARGLYSILEVALGLYLYSVAL
11   THRARGLYSGLNILEGLUGLUILEALALYS
12   THRLYSMETPROASPLEUASNALAASNSER
13   LEUGLUALAALAMETLYSILEILEGLUGLY
14   THRALALYSSERMETGLYILEGLUVALVAL
15   ASP

Entity 2, GAR RNA 60 residues - 19258 Da.

1050-1109, based on Escherichia coli 23S rRNA (U1061A)

1   GGGCAGGAUG
2   UAGGCUUAGA
3   AGCAGCCAUC
4   AUUUAAAGAA
5   AGCGUAAUAG
6   CUCACUGCCC

Entity 3, Thiostrepton 15 residues - 1665 Da.

1   TZOTHRTZBTSITZOXAAQUAILEALADHA
2   ALAXBBTZODHAPYT

Samples:

L11+RNA+thiostrepton: ribosomal L11 protein, [U->95% 2D; U->98% 15N; U->98% 13C], 0.3 mM; 23S GAR RNA 0.3 mM; Thiostrepton antibiotic 0.3 mM; potassium phosphate 20 mM; potassium chloride 200 mM; complete; superase

25C: pH: 6.1; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D (1H,15N)HSQCL11+RNA+thiostreptonnot available25C
3D HNCOL11+RNA+thiostreptonnot available25C
3D HNCAL11+RNA+thiostreptonnot available25C
3D HNCACBL11+RNA+thiostreptonnot available25C
IPAP (1H,15N)HSQCL11+RNA+thiostreptonnot available25C

Software:

NMRPipe, Delaglio et al. - processing

SPARKY v3, Goddard et al. - analysis & assignment

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker Avance 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts