BMRB Entry 7307

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7307

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Title:
Backbone assignment and RDCs of L11 in complex with RNA
Deposition date:
2006-09-28
Original release date:
2006-12-20
Authors:
Jonker, Henry; Ilin, Serge; Grimm, Steffen; Wohnert, Jens; Schwalbe, Harald
Citation:

Citation: Jonker, Henry; Ilin, Serge; Grimm, Steffen; Wohnert, Jens; Schwalbe, Harald. "L11 domain rearrangement upon binding to RNA and thiostrepton studied by NMR spectroscopy."  Nucleic Acids Res. 35, 441-454 (2007).
PubMed: 17169991

Assembly members:

Assembly members:
ribosomal L11 protein, polymer, 141 residues, 15088 Da.
23S GAR RNA, polymer, 60 residues, 19258 Da.

Natural source:

Natural source:   Common Name: Thermotoga maritima   Taxonomy ID: 2336   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermotoga maritima

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ribosomal L11 protein: MAKKVAAQIKLQLPAGKATP APPVGPALGQHGVNIMEFCK RFNAETADKAGMILPVVITV YEDKSFTFIIKTPPASFLLK KAAGIEKGSSEPKRKIVGKV TRKQIEEIAKTKMPDLNANS LEAAMKIIEGTAKSMGIEVV D
23S GAR RNA: GGGCAGGAUGUAGGCUUAGA AGCAGCCAUCAUUUAAAGAA AGCGUAAUAGCUCACUGCCC

Data sets:
Data typeCount
13C chemical shifts256
15N chemical shifts127
1H chemical shifts127
residual dipolar couplings127

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1L11 protein1
2GAR RNA2

Entities:

Entity 1, L11 protein 141 residues - 15088 Da.

1-141, Thermotoga maritima

1   METALALYSLYSVALALAALAGLNILELYS
2   LEUGLNLEUPROALAGLYLYSALATHRPRO
3   ALAPROPROVALGLYPROALALEUGLYGLN
4   HISGLYVALASNILEMETGLUPHECYSLYS
5   ARGPHEASNALAGLUTHRALAASPLYSALA
6   GLYMETILELEUPROVALVALILETHRVAL
7   TYRGLUASPLYSSERPHETHRPHEILEILE
8   LYSTHRPROPROALASERPHELEULEULYS
9   LYSALAALAGLYILEGLULYSGLYSERSER
10   GLUPROLYSARGLYSILEVALGLYLYSVAL
11   THRARGLYSGLNILEGLUGLUILEALALYS
12   THRLYSMETPROASPLEUASNALAASNSER
13   LEUGLUALAALAMETLYSILEILEGLUGLY
14   THRALALYSSERMETGLYILEGLUVALVAL
15   ASP

Entity 2, GAR RNA 60 residues - 19258 Da.

1050-1109, based on Escherichia coli 23S rRNA (U1061A)

1   GGGCAGGAUG
2   UAGGCUUAGA
3   AGCAGCCAUC
4   AUUUAAAGAA
5   AGCGUAAUAG
6   CUCACUGCCC

Samples:

L11+RNA: ribosomal L11 protein, [U->95% 2D; U->98% 15N; U->98% 13C], 0.3 mM; 23S GAR RNA 0.5 mM; potassium phosphate 20 mM; potassium chloride 200 mM; complete; superase

25C: pH: 6.1; temperature: 298 K

35C: pH: 6.1; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D (1H,15N)HSQCL11+RNAnot availablenot available
3D HNCOL11+RNAnot availablenot available
3D HNCAL11+RNAnot availablenot available

Software:

NMRPipe, Delaglio et al. - processing

SPARKY v3, Goddard et al. - analysis & assignment

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 5513
PDB
EMBL CAA77859
GB AAD35537 ABQ46490 ACB08837 ADA66341 AGL49376
REF NP_228264 WP_004081512 WP_011943108 WP_038033608 YP_001244066
SP A5IJW7 B1L939 P29395
AlphaFold A5IJW7 B1L939 P29395

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks