BMRB Entry 7303

Name: 1H, 13C and 15N resonance assignments for proapoptotic protein Nix (1~156) from Danio rerio
Published: 2007-06-26

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7303

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N resonance assignments for proapoptotic protein Nix (1~156) from Danio rerio

Deposition date:

2006-09-26

Original release date:

2007-06-26

Authors:

Chu, Feng-I; Ho, Yu; Hung, Chang-Jen; Tzou, Der-Lii, M

Citation:

Citation: Chu, Feng-I; Ho, Yu; Huang, Chang-Jen; Tzou, Der-Lii. "1H, 13C and 15N resonance assignments for proapoptotic protein Nix (residue 1 approximately 156) from Danio rerio" Biomol. NMR Assignments 1, 29-31 (2007).
PubMed: 19636819

Assembly members:

Assembly members:
15N Zebrafish NIX, polymer, 164 residues, 18039 Da.

Natural source:

Natural source: Common Name: Zebrafish Taxonomy ID: 7955 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Danio rerio

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
15N Zebrafish NIX: MSTTAAAQLNNNEEPGLNGS WVELEMNGNTQHQSPSLPSL LGNGNQGEPQASQALEAVQE EEEVLTGGLEHVPSSSSIHN GDMEKILLDAQHESSPSSSS CNSPPRPHSPDQDEGQIMFD VEMPSERDCQSEDDSMEKDK EDDILMNKGESWVADWFEHH HHHH

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	512
15N chemical shifts	129
1H chemical shifts	761

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NIX	1

Entities:

Entity 1, NIX 164 residues - 18039 Da.

1

MET

SER

THR

ALA

GLN

LEU

ASN

2

ASN

GLU

PRO

GLY

LEU

ASN

GLY

SER

3

TRP

VAL

GLU

LEU

GLU

MET

ASN

GLY

ASN

THR

4

GLN

HIS

GLN

SER

PRO

SER

LEU

PRO

SER

LEU

5

LEU

GLY

ASN

GLY

ASN

GLN

GLY

GLU

PRO

GLN

6

ALA

SER

GLN

ALA

LEU

GLU

ALA

VAL

GLN

GLU

7

GLU

VAL

LEU

THR

GLY

LEU

GLU

8

HIS

VAL

PRO

SER

ILE

HIS

ASN

9

GLY

ASP

MET

GLU

LYS

ILE

LEU

ASP

ALA

10

GLN

HIS

GLU

SER

PRO

SER

11

CYS

ASN

SER

PRO

ARG

PRO

HIS

SER

PRO

12

ASP

GLN

ASP

GLU

GLY

GLN

ILE

MET

PHE

ASP

13

VAL

GLU

MET

PRO

SER

GLU

ARG

ASP

CYS

GLN

14

SER

GLU

ASP

SER

MET

GLU

LYS

ASP

LYS

15

GLU

ASP

ILE

LEU

MET

ASN

LYS

GLY

GLU

16

SER

TRP

VAL

ALA

ASP

TRP

PHE

GLU

HIS

17

HIS

Samples:

Zebrafish_NIX_1: Zebrafish NIX, [U-90% 15N], 1.5 ± 0.1 mM

Zebrafish_NIX_2: Zebrafish NIX, [U-95% 13C; U-90% 15N], 1.5 ± 0.1 mM

pH_6.5_PBS_buffer: pH: 6.5; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N_HSQC	Zebrafish_NIX_1	not available	pH_6.5_PBS_buffer
HNCO	Zebrafish_NIX_2	not available	pH_6.5_PBS_buffer
HN(CA)CO	Zebrafish_NIX_2	not available	pH_6.5_PBS_buffer
HNCA	Zebrafish_NIX_2	not available	pH_6.5_PBS_buffer
HN(CO)CA	Zebrafish_NIX_2	not available	pH_6.5_PBS_buffer
CBCANH	Zebrafish_NIX_2	not available	pH_6.5_PBS_buffer
CBCA(CO)NH	Zebrafish_NIX_2	not available	pH_6.5_PBS_buffer
HCCCONH_C	Zebrafish_NIX_2	not available	pH_6.5_PBS_buffer
HCCCONH_H	Zebrafish_NIX_2	not available	pH_6.5_PBS_buffer
HBHA(CO)NH	Zebrafish_NIX_2	not available	pH_6.5_PBS_buffer
TOSCY-HSQC	Zebrafish_NIX_2	not available	pH_6.5_PBS_buffer

Software:

xwinnmr v3.0.b.13 - data processing

AURELIA v2.5.7 - data analysis

NMR spectrometers:

BRUKER AVANCE 600 MHz

GB	AAI63834 AAI63857 AAR83677
REF	NP_001012242