BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7298

Title: 1H, 13C, 15N-NMR resonance assignments for Znf-UBP domain of Ubp-M   PubMed: 17512543

Deposition date: 2006-09-20 Original release date: 2008-05-12

Authors: Pai, Ming-Tao; Zhou, Pei

Citation: Pai, Ming-Tao; Tzeng, Shiou-Ru; Kovacs, Jeffrey; Keaton, Mignon; Li, Shawn; Yao, Tso-Pang; Zhou, Pei. "Solution structure of the Ubp-M BUZ domain, a highly specific protein module that recognizes the C-terminal tail of free ubiquitin"  J. Mol. Biol. 370, 290-302 (2007).

Assembly members:
Ubp-M Znf-UBP domain, polymer, 126 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology' . 'E. coli

Entity Sequences (FASTA):
Ubp-M Znf-UBP domain: GSHMPVCRHIRKGLEQGNLK KALVNVEWNICQDCKTDNKV KDKAEEETEEKPSVWLCLKC GHQGCGRNSQEQHALKHYLT PRSEPHCLVLSLDNWSVWCY VCDNEVQYCSSNQLGQVVDY VRKQAS

Data sets:
Data typeCount
13C chemical shifts411
15N chemical shifts134
1H chemical shifts861

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Znf-UBP domain of Ubp-M1
2ZINC (II) ION, 12
3ZINC (II) ION, 22
4ZINC (II) ION, 32

Entities:

Entity 1, Znf-UBP domain of Ubp-M 126 residues - Formula weight is not available

1   GLYSERHISMETPROVALCYSARGHISILE
2   ARGLYSGLYLEUGLUGLNGLYASNLEULYS
3   LYSALALEUVALASNVALGLUTRPASNILE
4   CYSGLNASPCYSLYSTHRASPASNLYSVAL
5   LYSASPLYSALAGLUGLUGLUTHRGLUGLU
6   LYSPROSERVALTRPLEUCYSLEULYSCYS
7   GLYHISGLNGLYCYSGLYARGASNSERGLN
8   GLUGLNHISALALEULYSHISTYRLEUTHR
9   PROARGSERGLUPROHISCYSLEUVALLEU
10   SERLEUASPASNTRPSERVALTRPCYSTYR
11   VALCYSASPASNGLUVALGLNTYRCYSSER
12   SERASNGLNLEUGLYGLNVALVALASPTYR
13   VALARGLYSGLNALASER

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

15N_13C_labeled_1: Ubp-M Znf-UBP domain' '[U-13C; U-15N], 1, ; .; .; .

15N_13C_labeled_2: Ubp-M Znf-UBP domain' '[U-13C; U-15N], 1, ; .; .; .; .

sample_condition_1: pH: 7.0; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQC15N_13C_labeled_2not availablesample_condition_1
HNCAnot availablenot availablesample_condition_1
HN(CO)CAnot availablenot availablesample_condition_1
HN(CA)CBnot availablenot availablesample_condition_1
HN(COCA)CBnot availablenot availablesample_condition_1
13C-aliphatic-NOESY15N_13C_labeled_1not availablesample_condition_1
13C-aromatic-NOESYnot availablenot availablesample_condition_1
15N-NOESYnot availablenot availablesample_condition_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAD96401 BAD96604 BAE01147 BAG57569
EMBL CAB90432
GB AAD20949 AAH30777 AAR13293 ABM83321 ABM86536
REF NP_001001992 NP_001027582 NP_001247928 NP_006438 XP_001159952
SP Q4R6X7 Q9Y5T5

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts