BMRB Entry 7298
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7298
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Pai, Ming-Tao; Tzeng, Shiou-Ru; Kovacs, Jeffrey; Keaton, Mignon; Li, Shawn; Yao, Tso-Pang; Zhou, Pei. "Solution structure of the Ubp-M BUZ domain, a highly specific protein module that recognizes the C-terminal tail of free ubiquitin" J. Mol. Biol. 370, 290-302 (2007).
PubMed: 17512543
Assembly members:
Ubp-M Znf-UBP domain, polymer, 126 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Vector: pET15b
Entity Sequences (FASTA):
Ubp-M Znf-UBP domain: GSHMPVCRHIRKGLEQGNLK
KALVNVEWNICQDCKTDNKV
KDKAEEETEEKPSVWLCLKC
GHQGCGRNSQEQHALKHYLT
PRSEPHCLVLSLDNWSVWCY
VCDNEVQYCSSNQLGQVVDY
VRKQAS
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 411 |
| 15N chemical shifts | 134 |
| 1H chemical shifts | 861 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Znf-UBP domain of Ubp-M | 1 |
| 2 | ZINC (II) ION, 1 | 2 |
| 3 | ZINC (II) ION, 2 | 2 |
| 4 | ZINC (II) ION, 3 | 2 |
Entities:
Entity 1, Znf-UBP domain of Ubp-M 126 residues - Formula weight is not available
| 1 | GLY | SER | HIS | MET | PRO | VAL | CYS | ARG | HIS | ILE | ||||
| 2 | ARG | LYS | GLY | LEU | GLU | GLN | GLY | ASN | LEU | LYS | ||||
| 3 | LYS | ALA | LEU | VAL | ASN | VAL | GLU | TRP | ASN | ILE | ||||
| 4 | CYS | GLN | ASP | CYS | LYS | THR | ASP | ASN | LYS | VAL | ||||
| 5 | LYS | ASP | LYS | ALA | GLU | GLU | GLU | THR | GLU | GLU | ||||
| 6 | LYS | PRO | SER | VAL | TRP | LEU | CYS | LEU | LYS | CYS | ||||
| 7 | GLY | HIS | GLN | GLY | CYS | GLY | ARG | ASN | SER | GLN | ||||
| 8 | GLU | GLN | HIS | ALA | LEU | LYS | HIS | TYR | LEU | THR | ||||
| 9 | PRO | ARG | SER | GLU | PRO | HIS | CYS | LEU | VAL | LEU | ||||
| 10 | SER | LEU | ASP | ASN | TRP | SER | VAL | TRP | CYS | TYR | ||||
| 11 | VAL | CYS | ASP | ASN | GLU | VAL | GLN | TYR | CYS | SER | ||||
| 12 | SER | ASN | GLN | LEU | GLY | GLN | VAL | VAL | ASP | TYR | ||||
| 13 | VAL | ARG | LYS | GLN | ALA | SER |
Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
15N_13C_labeled_1: Ubp-M Znf-UBP domain, [U-13C; U-15N], 1.0 mM; phosphate 25 mM; KCl 100 mM; D2O 100%
15N_13C_labeled_2: Ubp-M Znf-UBP domain, [U-13C; U-15N], 1.0 mM; phosphate 25 mM; KCl 100 mM; D2O 5%; H2O 95%
sample_condition_1: pH: 7.0; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | 15N_13C_labeled_2 | not available | sample_condition_1 |
| HNCA | not available | not available | sample_condition_1 |
| HN(CO)CA | not available | not available | sample_condition_1 |
| HN(CA)CB | not available | not available | sample_condition_1 |
| HN(COCA)CB | not available | not available | sample_condition_1 |
| 13C-aliphatic-NOESY | 15N_13C_labeled_1 | not available | sample_condition_1 |
| 13C-aromatic-NOESY | not available | not available | sample_condition_1 |
| 15N-NOESY | not available | not available | sample_condition_1 |
Software:
No software information available
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAD96401 BAD96604 BAE01147 BAG57569 |
| EMBL | CAB90432 |
| GB | AAD20949 AAH30777 AAR13293 ABM83321 ABM86536 |
| REF | NP_001001992 NP_001027582 NP_001247928 NP_006438 XP_001159952 |
| SP | Q4R6X7 Q9Y5T5 |
| AlphaFold | Q4R6X7 Q9Y5T5 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks