BMRB Entry 7293

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7293
MolProbity Validation Chart

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Title:
Backbone 1H, 13C, and 15N chemical shift assignments for recoverin bound to rhodopsin kinase
Deposition date:
2006-09-11
Original release date:
2006-12-20
Authors:
Ames, James
Citation:

Citation: Ames, James; Levay, K.; Wingard, J.; Lusin, J.; Slepak, V.. "Structural Basis for Calcium-induced Inhibition of Rhodopsin Kinase by Recoverin"  J. Biol. Chem. 281, 37237-37245 (2006).
PubMed: 17020884

Assembly members:

Assembly members:
recoverin, polymer, 202 residues, 40.078 Da.
rk25, polymer, 25 residues, 40.078 Da.
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Bovine   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos Taurus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
recoverin: MGNSKSGALSKEILEELQLN TKFTEEELSSWYQSFLKECP SGRITRQEFQTIYSKFFPEA DPKAYAQHVFRSFDANSDGT LDFKEYVIALHMTSAGKTNQ KLEWAFSLYDVDGNGTISKN EVLEIVTAIFKMISPEDTKH LPEDENTPEKRAEKIWGFFG KKDDDKLTEKEFIEGTLANK EILRLIQFEPQKVKEKLKEK KL
rk25: MDFGSLETVVANSAFIAARG SFDAS

Data sets:
Data typeCount
13C chemical shifts383
15N chemical shifts187
1H chemical shifts376

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1recoverin1
2rk252
3CALCIUM (II) ION 13
4CALCIUM (II) ION 23

Entities:

Entity 1, recoverin 202 residues - 40.078 Da.

1   METGLYASNSERLYSSERGLYALALEUSER
2   LYSGLUILELEUGLUGLULEUGLNLEUASN
3   THRLYSPHETHRGLUGLUGLULEUSERSER
4   TRPTYRGLNSERPHELEULYSGLUCYSPRO
5   SERGLYARGILETHRARGGLNGLUPHEGLN
6   THRILETYRSERLYSPHEPHEPROGLUALA
7   ASPPROLYSALATYRALAGLNHISVALPHE
8   ARGSERPHEASPALAASNSERASPGLYTHR
9   LEUASPPHELYSGLUTYRVALILEALALEU
10   HISMETTHRSERALAGLYLYSTHRASNGLN
11   LYSLEUGLUTRPALAPHESERLEUTYRASP
12   VALASPGLYASNGLYTHRILESERLYSASN
13   GLUVALLEUGLUILEVALTHRALAILEPHE
14   LYSMETILESERPROGLUASPTHRLYSHIS
15   LEUPROGLUASPGLUASNTHRPROGLULYS
16   ARGALAGLULYSILETRPGLYPHEPHEGLY
17   LYSLYSASPASPASPLYSLEUTHRGLULYS
18   GLUPHEILEGLUGLYTHRLEUALAASNLYS
19   GLUILELEUARGLEUILEGLNPHEGLUPRO
20   GLNLYSVALLYSGLULYSLEULYSGLULYS
21   LYSLEU

Entity 2, rk25 25 residues - 40.078 Da.

RK25 is the first 25 N-terminal residues of rhodopsin kinase

1   METASPPHEGLYSERLEUGLUTHRVALVAL
2   ALAASNSERALAPHEILEALAALAARGGLY
3   SERPHEASPALASER

Entity 3, CALCIUM (II) ION 1 - Ca - 40.078 Da.

1   CA

Samples:

sample_1: recoverin, [U-95% 13C; U-90% 15N], 0.5 mM; rk25, [U-95%; U-90% 15N], 0.5 mM

conditions_1: ionic strength: 20 mM; pH: 7.0; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
HNCOsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCACONHsample_1not availableconditions_1
HNCOCACBsample_1not availableconditions_1
HBHACONHsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance DRX 600 MHz

Related Database Links:

PDB
GB AAA30752 ELR45456
REF NP_776598 XP_001364656 XP_003765754 XP_005910585 XP_005963795
SP P28327
TPG DAA23691
AlphaFold P28327

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks