BMRB Entry 7276

Name: Complete 1H, 13C and 15N resonance assignments of Blo t 5, a major mite allergen from Blomia tropicalis.
Published: 2007-01-10

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PDB ID: 2mey
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7276
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Complete 1H, 13C and 15N resonance assignments of Blo t 5, a major mite allergen from Blomia tropicalis.

Deposition date:

2006-08-27

Original release date:

2007-01-10

Authors:

Naik, Mandar; Chang, Chi-Fon; Huang, Tai-Huang

Citation:

Citation: Naik, Mandar; Chang, Chi-Fon; Kuo, I-Chun; Yu, Tsunai; Fang, Pei-Ju; Chua, Kaw-Yan; Huang, Tai-Huang. "Complete 1H, 13C and 15N resonance assignments of Blo t 5, a major mite allergen from Blomia tropicalis." J. Biomol. NMR 38, 189-189 (2006).
PubMed: 17206470

Assembly members:

Assembly members:
Blo t 5, polymer, 119 residues, 14035.8 Da.

Natural source:

Natural source: Common Name: House dust mite Taxonomy ID: 40697 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: mites and ticks Blomia tropicalis

Experimental source:

Experimental source: Production method: recombinant technology Vector: pGEX4T-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Blo t 5: GSQEHKPKKDDFRNEFDHLL IEQANHAIEKGEHQLLYLQH QLDELNENKSKELQEKIIRE LDVVCAMIEGAQGALERELK RTDLNILERFNYEEAQTLSK ILLKDLKETEQKVKDIQTQ

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	595
15N chemical shifts	142
1H chemical shifts	903

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Blo t 5	1

Entities:

Entity 1, Blo t 5 119 residues - 14035.8 Da.

-2 GLY, -1 SER, 1 GLN, 2 GLU, ..., 117 GLN

1

GLY

SER

GLN

GLU

HIS

LYS

PRO

LYS

ASP

2

ASP

PHE

ARG

ASN

GLU

PHE

ASP

HIS

LEU

3

ILE

GLU

GLN

ALA

ASN

HIS

ALA

ILE

GLU

LYS

4

GLY

GLU

HIS

GLN

LEU

TYR

LEU

GLN

HIS

5

GLN

LEU

ASP

GLU

LEU

ASN

GLU

ASN

LYS

SER

6

LYS

GLU

LEU

GLN

GLU

LYS

ILE

ARG

GLU

7

LEU

ASP

VAL

CYS

ALA

MET

ILE

GLU

GLY

8

ALA

GLN

GLY

ALA

LEU

GLU

ARG

GLU

LEU

LYS

9

ARG

THR

ASP

LEU

ASN

ILE

LEU

GLU

ARG

PHE

10

ASN

TYR

GLU

ALA

GLN

THR

LEU

SER

LYS

11

ILE

LEU

LYS

ASP

LEU

LYS

GLU

THR

GLU

12

GLN

LYS

VAL

LYS

ASP

ILE

GLN

THR

GLN

Samples:

sample_1: Blo t 5, [U-13C; U-15N], 1 ± 0.05 mM; Potassium Phosphate 50 ± 0.01 mM; Sodium Chloride 100 ± 0.01 mM; EDTA 2 ± 0.01 mM; Sodium Azide 0.001 ± 0.0001 %

sample_2: Blo t 5, [U-15N], 1 ± 0.05 mM; Potassium Phosphate 50 ± 0.01 mM; Sodium Chloride 100 ± 0.01 mM; EDTA 2 ± 0.01 mM; Sodium Azide 0.001 ± 0.0001 %

sample_3: Blo t 5 1 ± 0.05 mM; Potassium Phosphate 50 ± 0.01 mM; Sodium Chloride 100 ± 0.01 mM; EDTA 2 ± 0.01 mM; Sodium Azide 0.001 ± 0.0001 %

sample_4: Blo t 5, [U-10% 13C], 0.8 ± 0.05 mM; Potassium Phosphate 50 ± 0.01 mM; Sodium Chloride 100 ± 0.01 mM; EDTA 2 ± 0.01 mM; Sodium Azide 0.001 ± 0.0001 %

sample_5: Blo t 5, [15N]-Lys,Leu, 0.8 ± 0.05 mM; Potassium Phosphate 50 ± 0.01 mM; Sodium Chloride 100 ± 0.01 mM; EDTA 2 ± 0.01 mM; Sodium Azide 0.001 ± 0.0001 %

ph7.5_295K: pH: 7.5; temperature: 295 K

Experiments:

Name	Sample	Sample state	Sample conditions
15N HSQC	sample_2	not available	ph7.5_295K
13C HSQC	sample_1	not available	ph7.5_295K
HNCa	sample_1	not available	ph7.5_295K
HN(CO)Ca	sample_1	not available	ph7.5_295K
HNCaCb	sample_1	not available	ph7.5_295K
CbCa(CO)NH	sample_1	not available	ph7.5_295K
HNCO	sample_1	not available	ph7.5_295K
HN(Ca)CO	sample_1	not available	ph7.5_295K
HCaCO	sample_1	not available	ph7.5_295K
HbHa(CO)NH	sample_1	not available	ph7.5_295K
(H)C(CO)NH	sample_1	not available	ph7.5_295K
H(CCO)NH	sample_1	not available	ph7.5_295K
15N_TocsyHSQC	sample_1	not available	ph7.5_295K
HCCH-Cosy	sample_1	not available	ph7.5_295K
CCH-Tocsy	sample_1	not available	ph7.5_295K
HCCH-Tocsy	sample_1	not available	ph7.5_295K
Cosy	sample_3	not available	ph7.5_295K
15N edited NoesyHSQC	not available	not available	ph7.5_295K
13C edited NoesyHSQC	not available	not available	ph7.5_295K
Tocsy	sample_3	not available	ph7.5_295K
Noesy	sample_3	not available	ph7.5_295K
(Hb)Cb(CgCd)Hd	sample_1	not available	ph7.5_295K
(Hb)Cb(CgCdCe)He	sample_1	not available	ph7.5_295K
15N HMBC	sample_2	not available	ph7.5_295K
15N HMQC	sample_2	not available	ph7.5_295K
HNHa	sample_1	not available	ph7.5_295K
13C CT-HSQC	sample_4	not available	ph7.5_295K

Software:

xwinnmr v3.5, Bruker - NMR data acquisition, processing

SPARKY v3.112, UCSF - NMR data analysis

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 500 MHz
Bruker Avance 800 MHz

BMRB	15345
PDB	2JMH 2JRK 2MEY
GB	AAD10850 ABH06351 ABH06352 ABH06353 ABH06354
SP	O96870
AlphaFold	O96870