BMRB Entry 7263

Name: Complete 1H assignment of Meg-A12
Published: 2007-03-09

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PDB ID: 2i1p
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7263
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Complete 1H assignment of Meg-A12

Deposition date:

2006-08-14

Original release date:

2007-03-09

Authors:

Wolf, Christian; Dancea, Felician; Shi, Meichen; Bade-Noskova, Veronika; Rueterjans, Heinz; Kerjaschki, Dontscho; Luecke, Christian

Citation:

Citation: Wolf, Christian; Dancea, Felician; Shi, Meichen; Bade-Noskova, Veronika; Rueterjans, Heinz; Kerjaschki, Dontscho; Luecke, Christian. "Solution structure of the twelfth cysteine-rich ligand-binding repeat in rat megalin" J. Biomol. NMR 37, 321-328 (2007).
PubMed: 17245526

Assembly members:

Assembly members:
Meg-A12, polymer, 48 residues, 5092.67 Da.
CA, non-polymer, 40.078 Da.

Natural source:

Natural source: Common Name: Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Vector: pKM263

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Meg-A12: GAMVLNCTSAQFKCADGSSC INSRYRCDGVYDCRDNSDEA GCPTRPPG

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
1H chemical shifts	280

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Meg-A12	1
2	CALCIUM (II) ION	2

Entities:

Entity 1, Meg-A12 48 residues - 5092.67 Da.

the first three residues are cloning artifacts

1

GLY

ALA

MET

VAL

LEU

ASN

CYS

THR

SER

ALA

2

GLN

PHE

LYS

CYS

ALA

ASP

GLY

SER

CYS

3

ILE

ASN

SER

ARG

TYR

ARG

CYS

ASP

GLY

VAL

4

TYR

ASP

CYS

ARG

ASP

ASN

SER

ASP

GLU

ALA

5

GLY

CYS

PRO

THR

ARG

PRO

GLY

Entity 2, CALCIUM (II) ION - Ca - 40.078 Da.

1

CA

Samples:

sample_1: Meg-A12 1.5 mM

conditions_1: pH: 5.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H1H TOCSY	sample_1	not available	conditions_1
1H1H NOESY	sample_1	not available	conditions_1

Software:

xwinnmr v2.6, BRUKER - Data collection

AURELIA v2.5.9, BRUKER - Data analysis

NMR spectrometers:

BRUKER DMX 600 MHz