BMRB Entry 7257

Name: Solution structure of the RRM domain of SR rich factor 9G8
Published: 2006-12-20

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PDB ID: 2hvz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR7257
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the RRM domain of SR rich factor 9G8

Deposition date:

2006-08-10

Original release date:

2006-12-20

Authors:

Tintaru, A.; Hautbergue, G.; Golovanov, A.; Lian, L.; Wilson, S.

Citation:

Citation: Hargous, Y.; Hautbergue, G.; Tintaru, A.; Skrisovska, L.; Golovanov, A.; Stevenin, J.; Lian, L.; Wilson, S.; Allain, F.. "Molecular basis of RNA recognition and TAP binding by the SR proteins SRp20 and 9G8" EMBO J. 25, 5126-5137 (2006).
PubMed: 17036044

Assembly members:

Assembly members:
RRM domain of SR rich factor 9G8, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RRM domain of SR rich factor 9G8: MKVYVGNLGTGAGKGELERA FSYYGPLRTVWIARNPPGFA FVEFEDPRDAEDAVRGLDGK VICGSRVRVELSTGMPRRSR FDRPPARRKLLEVLFNGPLE H

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	444
15N chemical shifts	109
1H chemical shifts	697

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RRM domain of SR rich factor 9G8	1

Entities:

Entity 1, RRM domain of SR rich factor 9G8 101 residues - Formula weight is not available

1

MET

LYS

VAL

TYR

VAL

GLY

ASN

LEU

GLY

THR

2

GLY

ALA

GLY

LYS

GLY

GLU

LEU

GLU

ARG

ALA

3

PHE

SER

TYR

GLY

PRO

LEU

ARG

THR

VAL

4

TRP

ILE

ALA

ARG

ASN

PRO

GLY

PHE

ALA

5

PHE

VAL

GLU

PHE

GLU

ASP

PRO

ARG

ASP

ALA

6

GLU

ASP

ALA

VAL

ARG

GLY

LEU

ASP

GLY

LYS

7

VAL

ILE

CYS

GLY

SER

ARG

VAL

ARG

VAL

GLU

8

LEU

SER

THR

GLY

MET

PRO

ARG

SER

ARG

9

PHE

ASP

ARG

PRO

ALA

ARG

LYS

LEU

10

LEU

GLU

VAL

LEU

PHE

ASN

GLY

PRO

LEU

GLU

11

HIS

Samples:

sample_1: RRM domain of SR rich factor 9G8, [U-13C; U-15N], 0.6 mM; CH3COONa 20 mM; L-ARG 25 mM; L-Glu 50 mM; EDTA 5 mM; DTT 10 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 5.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 13C-separated NOESY	not available	not available	not available
3D 15N-separated NOESY	not available	not available	not available
2D TOCSY	not available	not available	not available

Software:

xwinnmr v3.5 - collection

NMRPipe v1.0 - processing

NMRView v5.01 - data analysis

CYANA v2.1 - structure solution

AMBER v7.0 - refinement

NMR spectrometers:

Bruker DRX 600 MHz

PDB	2HVZ
DBJ	BAC03661 BAC28058 BAC32521 BAC38650 BAE01828
GB	AAA35495 AAA88098 AAH00997 AAH14857 AAH17369
REF	NP_001026854 NP_001029449 NP_001034124 NP_001086749 NP_001182375
SP	Q16629 Q3T106 Q8BL97
TPG	DAA24684
AlphaFold	Q16629 Q3T106 Q8BL97