BMRB Entry 7246
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7246
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NMR-STAR v3 text file.
XML gzip file.
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Citation: Perez-Alvarado, Gabriela; Munnerlyn, Audrey; Dyson, H.; Grosschedl, Rudolf; Wright, Peter. "Identification of the regions involved in DNA binding by the mouse PEBP2alpha protein" FEBS Lett. 470, 125-130 (2000).
PubMed: 10734220
Assembly members:
PEBP2alpha DNA binding domain, polymer, 136 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Vector: pet21d
Entity Sequences (FASTA):
PEBP2alpha DNA binding domain: DNRTMVEIIADHPAELVRTD
SPNFLCSVLPSHWRCNKTLP
VAFKVVALGEVPDGTVVTVM
AGNDENYSAELRNASAVMKN
QVARFNDLRFVGRSGRGKSF
TLTITVFTNPPQVATYHRAI
KVTVDGPREPRRHRQK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 96 |
| 15N chemical shifts | 94 |
| 1H chemical shifts | 94 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PEBP2alpha DNA binding domain | 1 |
Entities:
Entity 1, PEBP2alpha DNA binding domain 136 residues - Formula weight is not available
| 1 | ASP | ASN | ARG | THR | MET | VAL | GLU | ILE | ILE | ALA | ||||
| 2 | ASP | HIS | PRO | ALA | GLU | LEU | VAL | ARG | THR | ASP | ||||
| 3 | SER | PRO | ASN | PHE | LEU | CYS | SER | VAL | LEU | PRO | ||||
| 4 | SER | HIS | TRP | ARG | CYS | ASN | LYS | THR | LEU | PRO | ||||
| 5 | VAL | ALA | PHE | LYS | VAL | VAL | ALA | LEU | GLY | GLU | ||||
| 6 | VAL | PRO | ASP | GLY | THR | VAL | VAL | THR | VAL | MET | ||||
| 7 | ALA | GLY | ASN | ASP | GLU | ASN | TYR | SER | ALA | GLU | ||||
| 8 | LEU | ARG | ASN | ALA | SER | ALA | VAL | MET | LYS | ASN | ||||
| 9 | GLN | VAL | ALA | ARG | PHE | ASN | ASP | LEU | ARG | PHE | ||||
| 10 | VAL | GLY | ARG | SER | GLY | ARG | GLY | LYS | SER | PHE | ||||
| 11 | THR | LEU | THR | ILE | THR | VAL | PHE | THR | ASN | PRO | ||||
| 12 | PRO | GLN | VAL | ALA | THR | TYR | HIS | ARG | ALA | ILE | ||||
| 13 | LYS | VAL | THR | VAL | ASP | GLY | PRO | ARG | GLU | PRO | ||||
| 14 | ARG | ARG | HIS | ARG | GLN | LYS |
Samples:
sample_1: PEBP2alpha DNA binding domain, [U-2H; U-15N; U-13C], 0.2 mM; Tris HCl-d8 20 mM; K2SO4 20 mM; KCl 100 mM; DTT 10 mM; D2O 5%
conditions_1: pH: 6.5; temperature: 295 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | sample_1 | not available | conditions_1 |
| 1H15N_NOESY_HSQC | sample_1 | not available | conditions_1 |
| CT_HNCA | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
Software:
NMRPipe, NIH - processing
Felix97, Molecular Simulations - processing
NMRDraw, NIH - analysis
NMRView, OneMoonScientific - analysis
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| DBJ | BAA03485 BAA03486 BAB40441 BAG61865 BAK62687 |
| GB | AAA89072 AAB65158 AAB65159 AAB65409 AAB82419 |
| REF | NP_001015051 NP_001019801 NP_001139392 NP_001139510 NP_001258556 |
| SP | Q08775 Q13950 Q9Z2J9 |
| TPG | DAA16468 DAA16469 |
| AlphaFold | Q08775 Q13950 Q9Z2J9 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks