BMRB Entry 7245

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7245

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Title:
Structural and Functional Characterization of TM VII of the NHE1 Isoform of the Na+/H+ Exchanger
Deposition date:
2006-07-25
Original release date:
2006-08-12
Authors:
Rainey, Jan; Ding, Jie; Xu, Carolyn; Fliegel, Larry; Sykes, Brian
Citation:

Citation: Ding, Jie; Rainey, Jan; Xu, Carolyn; Sykes, Brian; Fliegel, Larry. "Structural and functional characterization of transmembrane segment VII of the Na+/H+ exchanger isoform 1."  J. Biol. Chem. 281, 29817-29829 (2006).
PubMed: 16861220

Assembly members:

Assembly members:
TM VII of Na+/H+ exchanger, polymer, 28 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TM VII of Na+/H+ exchanger: HINELLHILVFGESLLNDAV TVVLYKKX

Data sets:
Data typeCount
13C chemical shifts69
15N chemical shifts6
1H chemical shifts226

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TM VII of Na+/H+ exchanger1

Entities:

Entity 1, TM VII of Na+/H+ exchanger 28 residues - Formula weight is not available

1   HISILEASNGLULEULEUHISILELEUVAL
2   PHEGLYGLUSERLEULEUASNASPALAVAL
3   THRVALVALLEUTYRLYSLYSNH2

Samples:

sample_1: TM VII, none, 1 mM; DPC, [U-2H], 75 mM; DSS 1 mM

sample_2: TM VII, [15N]-L5,L9,G12,L15,A19,L24, 1 mM; DPC, [U-2H], 75 mM; DSS 1 mM

conditions_1: pH: 4.8; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HSQC (natural abundance)not availablenot availableconditions_1
NOESYnot availablenot availableconditions_1
TOCSYnot availablenot availableconditions_1
15N-1H HSQCnot availablenot availableconditions_1
15N-edited NOESY-HSQC (at 500 MHz)not availablenot availableconditions_1
15N-edited TOCSY-HSQC (at 500 MHz)not availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 500 MHz

Related Database Links:

BMRB 19484
PDB
DBJ BAE02258 BAE29332 BAE32882 BAE38221 BAE75800
EMBL CAA42558 CAA43721 CAA48771 CAA69925 CAB45232
GB AAA31092 AAA91483 AAA92976 AAA98479 AAB20633
REF NP_001007104 NP_001038108 NP_001081553 NP_001095191 NP_001106952
SP P19634 P23791 P26431 P48761 P48762
TPG DAA32066
AlphaFold P19634 P23791 P26431 P48761 P48762

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks