BMRB Entry 7243
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7243
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Ghasriani, Houman; Teilum, Kaare; Johnsson, Ylva; Fernlund, Per; Drakenberg, Torbjorn. "Solution structures of human and porcine beta-microseminoprotein" J. Mol. Biol. 362, 502-515 (2006).
PubMed: 16930619
Assembly members:
beta-microseminoprotein, polymer, 94 residues, Formula weight is not available
Natural source: Common Name: Porcine Taxonomy ID: 9823 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: sus scorfa
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
beta-microseminoprotein: GGGQCYFIPNQSLKPNECQD
LKGVSHPLNSVWKTKDCEEC
TCGQDAISCCNTAAIPTGYD
TNKCQKILNKKTCTYTVVEK
KDPGKTCDVTGWVL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 322 |
| 15N chemical shifts | 98 |
| 1H chemical shifts | 581 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | pMSP | 1 |
Entities:
Entity 1, pMSP 94 residues - Formula weight is not available
| 1 | GLY | GLY | GLY | GLN | CYS | TYR | PHE | ILE | PRO | ASN | ||||
| 2 | GLN | SER | LEU | LYS | PRO | ASN | GLU | CYS | GLN | ASP | ||||
| 3 | LEU | LYS | GLY | VAL | SER | HIS | PRO | LEU | ASN | SER | ||||
| 4 | VAL | TRP | LYS | THR | LYS | ASP | CYS | GLU | GLU | CYS | ||||
| 5 | THR | CYS | GLY | GLN | ASP | ALA | ILE | SER | CYS | CYS | ||||
| 6 | ASN | THR | ALA | ALA | ILE | PRO | THR | GLY | TYR | ASP | ||||
| 7 | THR | ASN | LYS | CYS | GLN | LYS | ILE | LEU | ASN | LYS | ||||
| 8 | LYS | THR | CYS | THR | TYR | THR | VAL | VAL | GLU | LYS | ||||
| 9 | LYS | ASP | PRO | GLY | LYS | THR | CYS | ASP | VAL | THR | ||||
| 10 | GLY | TRP | VAL | LEU |
Samples:
sample_1: beta-microseminoprotein 4.0 ± 0.2 mM; H2O 90%; D2O 10%
sample_2: beta-microseminoprotein, [U-15N], 3.4 – 4.2 mM; H2O 90%; D2O 10%
sample_3: beta-microseminoprotein, [U-13C; U-15N], 3.4 – 4.2 mM; H2O 90%; D2O 10%
conditions_1: pH: 6.0; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | sample_1 | not available | conditions_1 |
| HNCA | sample_1 | not available | conditions_1 |
| HNCO | sample_1 | not available | conditions_1 |
| CbCACONH | sample_1 | not available | conditions_1 |
| 2D-TOCSY | sample_1 | not available | conditions_1 |
Software:
software_1 -
NMR spectrometers:
- Varian Unity 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks