BMRB Entry 7242

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7242

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Title:
13Ca resonances (100%), 1Ha(99.6%), non-proline backbone 1HN and 15N (94%), and side-chain 13C and 1H (85%) chemical shift assignment for URO-synthase
Deposition date:
2006-07-24
Original release date:
2007-11-21
Authors:
Cunha, Luis; Kuti, Miklos; Bishop, David; Mezei, Mihaly; Zeng, Lei; Zhou, Ming-Ming; Desnick, Robert
Citation:

Citation: Cunha, Luis; Kuti, Miklos; Bishop, David; Mezei, Mihaly; Zeng, Lei; Zhou, Ming-Ming; Desnick, Robert. "Human uroporphyrinogen III synthase: NMR-based mapping of the active site"  Proteins 71, 855-873 (2008).
PubMed: 18004775

Assembly members:

Assembly members:
Uroporphyrinogen-III-synthase, polymer, 266 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Ulp1 yeast   Taxonomy ID: 4932   Superkingdom: Eubacteria   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Uroporphyrinogen-III-synthase: SMKVLLLKDAKEDDCGQDPY IRELGLYGLEATLIPVLSFE FLSLPSFSEKLSHPEDYGGL IFTSPRAVEAAELCLEQNNK TEVWERSLKEKWNAKSVYVV GNATASLVSKIGLDTEGETC GNAEKLAEYICSRESSALPL LFPCGNLKREILPKALKDKG IAMESITVYQTVAHPGIQGN LNSYYSQQGVPASITFFSPS GLTYSLKHIQELSGDNIDQI KFAAIGPTTARALAAQGLPV SCTAESPTPQALATGIRKAL QPHGCC

Data sets:
Data typeCount
13C chemical shifts789
15N chemical shifts241
1H chemical shifts1531

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Uroporphyrinogen-III-Synthase (URO-synthase)1

Entities:

Entity 1, Uroporphyrinogen-III-Synthase (URO-synthase) 266 residues - Formula weight is not available

1   SERMETLYSVALLEULEULEULYSASPALA
2   LYSGLUASPASPCYSGLYGLNASPPROTYR
3   ILEARGGLULEUGLYLEUTYRGLYLEUGLU
4   ALATHRLEUILEPROVALLEUSERPHEGLU
5   PHELEUSERLEUPROSERPHESERGLULYS
6   LEUSERHISPROGLUASPTYRGLYGLYLEU
7   ILEPHETHRSERPROARGALAVALGLUALA
8   ALAGLULEUCYSLEUGLUGLNASNASNLYS
9   THRGLUVALTRPGLUARGSERLEULYSGLU
10   LYSTRPASNALALYSSERVALTYRVALVAL
11   GLYASNALATHRALASERLEUVALSERLYS
12   ILEGLYLEUASPTHRGLUGLYGLUTHRCYS
13   GLYASNALAGLULYSLEUALAGLUTYRILE
14   CYSSERARGGLUSERSERALALEUPROLEU
15   LEUPHEPROCYSGLYASNLEULYSARGGLU
16   ILELEUPROLYSALALEULYSASPLYSGLY
17   ILEALAMETGLUSERILETHRVALTYRGLN
18   THRVALALAHISPROGLYILEGLNGLYASN
19   LEUASNSERTYRTYRSERGLNGLNGLYVAL
20   PROALASERILETHRPHEPHESERPROSER
21   GLYLEUTHRTYRSERLEULYSHISILEGLN
22   GLULEUSERGLYASPASNILEASPGLNILE
23   LYSPHEALAALAILEGLYPROTHRTHRALA
24   ARGALALEUALAALAGLNGLYLEUPROVAL
25   SERCYSTHRALAGLUSERPROTHRPROGLN
26   ALALEUALATHRGLYILEARGLYSALALEU
27   GLNPROHISGLYCYSCYS

Samples:

sample_1: Uroporphyrinogen-III-synthase, [U-2H; U-13C; U-15N], 0.7 mM; NaH2PO4 buffer 20 mM; NaCl 100 mM; EDTA 1 mM; perdeuterated DTT 2 mM; H2O 90%; D2O 10%

sample_2: Uroporphyrinogen-III-synthase, [U-15N], 0.7 mM; NaH2PO4 buffer 20 mM; NaCl 100 mM; EDTA 1 mM; perdeuterated DTT 2 mM; H2O 90%; D2O 10%

sample_3: Uroporphyrinogen-III-synthase, [U-13C], 0.7 mM; NaH2PO4 buffer 20 mM; NaCl 100 mM; EDTA 1 mM; perdeuterated DTT 2 mM; H2O 90%; D2O 10%

sample_4: Uroporphyrinogen-III-synthase, [U-2H; U-13C; U-15N]-{I(d1-13CH3),L(13CH3,12CD3),V(13CH3,12CD3)}, 0.7 mM; NaH2PO4 buffer 20 mM; NaCl 100 mM; EDTA 1 mM; perdeuterated DTT 2 mM; H2O 90%; D2O 10%

sample_5: Uroporphyrinogen-III-synthase, [U-2H; U-15N]-{I(d1-13CH3),L(13CH3,13CH3),V(13CH3,13CH3),F(1H),Y(1H)}, 0.7 mM; NaH2PO4 buffer 20 mM; NaCl 100 mM; EDTA 1 mM; perdeuterated DTT 2 mM; H2O 90%; D2O 10%

sample_6: Uroporphyrinogen-III-synthase, [10% 13C], 0.7 mM; NaH2PO4 buffer 20 mM; NaCl 100 mM; EDTA 1 mM; perdeuterated DTT 2 mM; H2O 90%; D2O 10%

conditions_1: pH: 7.45; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
HNCACBnot availablenot availableconditions_1
HNCOCACBnot availablenot availableconditions_1
3D (H)C(CO)NH-TOCSYnot availablenot availableconditions_1
3D H(CCO)NH-TOCSYnot availablenot availableconditions_1
15N-TOCSY-HSQCnot availablenot availableconditions_1
15N-NOESY-HSQCnot availablenot availableconditions_1
13C-HSQC-NOESYnot availablenot availableconditions_1
2D-TOCSYnot availablenot availableconditions_1
2D-NOESYnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
DBJ BAG37410
GB AAA60273 AAB21242 AAG36795 AAH02573 ABM82862
REF NP_000366 NP_001253711 XP_002756746 XP_002821304 XP_003277827
SP P10746
AlphaFold P10746

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks