BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7229

Title: Solution structure of Brak/CXCL14   PubMed: 16987528

Deposition date: 2006-07-14 Original release date: 2007-01-04

Authors: Volkman, B.; Peterson, F.; Schwarze, S.; Harder, A.

Citation: Peterson, F.; Thorpe, J.; Harder, A.; Volkman, B.; Schwarze, S.. "Structural Determinants Involved in the Regulation of CXCL14/BRAK Expression by the 26 S Proteasome"  J. Mol. Biol. 363, 813-822 (2006).

Assembly members:
Small inducible cytokine B14, polymer, 78 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology' 'Escherichia coli' 'Escherichia coli

Entity Sequences (FASTA):
Small inducible cytokine B14: GSKCKCSRKGPKIRYSDVKK LEMKPKYPHCEEKMVIITTK SVSRYRGQEHCLHPKLQSTK RFIKWYNAWNEKRRVYEE

Data sets:
Data typeCount
13C chemical shifts325
15N chemical shifts71
1H chemical shifts518

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Small inducible cytokine B141

Entities:

Entity 1, Small inducible cytokine B14 78 residues - Formula weight is not available

1   GLYSERLYSCYSLYSCYSSERARGLYSGLY
2   PROLYSILEARGTYRSERASPVALLYSLYS
3   LEUGLUMETLYSPROLYSTYRPROHISCYS
4   GLUGLULYSMETVALILEILETHRTHRLYS
5   SERVALSERARGTYRARGGLYGLNGLUHIS
6   CYSLEUHISPROLYSLEUGLNSERTHRLYS
7   ARGPHEILELYSTRPTYRASNALATRPASN
8   GLULYSARGARGVALTYRGLUGLU

Samples:

sample_1: Small inducible cytokine B14' '[U-15N; U-13C]; .; .; .; .

sample_cond_1: ionic strength: 300 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
3D 13C-separated NOESY (AROMATIC)not availablenot availablenot available

Software:

xwinnmr v3.5 - collection

NMRPipe v2004 - processing

SPSCAN v1.1.0 - data analysis

XEASY v1.3 - data analysis

GARANT v2.1 - data analysis

CYANA v2.1 - structure solution

X-PLOR NIH v2.9.3 - refinement

NMR spectrometers:

  • Bruker DRX 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts