BMRB Entry 7225

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PDB ID: 2hep
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7225
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of the UPF0291 protein ynzC from Bacillus subtilis. Northeast Structural Genomics target SR384. (CASP Target)

Deposition date:

2006-07-14

Original release date:

2008-07-16

Authors:

Aramini, J.; Swapna, G.; Ho, C.; Shetty, K.; Cunningham, K.; Ma, L.-C.; Xiao, R.; Liu, J.; Baran, M.; Acton, T.; Rost, B.; Montelione, G.

Citation:

Citation: Aramini, J.; Sharma, S.; Huang, Y.; Swapna, G.; Ho, C.; Shetty, K.; Cunningham, K.; Ma, L.-C.; Zhao, L.; Owens, L.; Jiang, M.; Xiao, R.; Liu, J.; Baran, M.; Acton, T.; Rost, B.; Montelione, G.. "Solution NMR structure of the SOS response protein YnzC from Bacillus subtilis" Proteins 72, 526-530 (2008).
PubMed: 18431750

Assembly members:

Assembly members:
UPF0291 protein ynzC, polymer, 85 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Bacillus subtilis Taxonomy ID: 1423 Superkingdom: Bacteria Kingdom: not available Genus/species: Bacillus subtilis

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UPF0291 protein ynzC: MISNAKIARINELAAKAKAG VITEEEKAEQQKLRQEYLKG FRSSMKNTLKSVKIIDPEGN DVTPEKLKREQRNNKLHLEH HHHHH

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	338
15N chemical shifts	75
1H chemical shifts	489

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UPF0291 protein ynzC	1

Entities:

Entity 1, UPF0291 protein ynzC 85 residues - Formula weight is not available

1

MET

ILE

SER

ASN

ALA

LYS

ILE

ALA

ARG

ILE

2

ASN

GLU

LEU

ALA

LYS

ALA

LYS

ALA

GLY

3

VAL

ILE

THR

GLU

LYS

ALA

GLU

GLN

4

GLN

LYS

LEU

ARG

GLN

GLU

TYR

LEU

LYS

GLY

5

PHE

ARG

SER

MET

LYS

ASN

THR

LEU

LYS

6

SER

VAL

LYS

ILE

ASP

PRO

GLU

GLY

ASN

7

ASP

VAL

THR

PRO

GLU

LYS

LEU

LYS

ARG

GLU

8

GLN

ARG

ASN

LYS

LEU

HIS

LEU

GLU

HIS

9

HIS

Samples:

sample_1: UPF0291 protein ynzC, [U-13C; U-15N], 1.12 mM; MES 20 mM; NaCl 100 mM; CaCl2 5 mM; DTT 10 mM; NaN3 0.02%; D2O 5%; H2O 95%

sample_2: UPF0291 protein ynzC, [U-5% 13C; U-15N], 1.12 mM; MES 20 mM; NaCl 100 mM; CaCl2 5 mM; DTT 10 mM; NaN3 0.02%; D2O 5%; H2O 95%

sample_cond_1: ionic strength: 100 mM; pH: 6.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-separated NOESY	not available	not available	not available
3D 13C-separated NOESY	not available	not available	not available
HNHA	not available	not available	not available
3D GFT-CBCACAcoNHN	not available	not available	not available
GFT-HNNCACBCA	not available	not available	not available
GFT-HABCABcoNHN	not available	not available	not available
3D HCCH-TOCSY	not available	not available	not available
CCcoNH TOCSY	not available	not available	not available
HCCH-COSY	not available	not available	not available
high resolution 2D CH-HQSC (for stereospecific assignment of Val/Leu methyls)	not available	not available	not available
3D HNCO	not available	not available	not available
HNcaCO	not available	not available	not available
2D 15N1H-heteronuclear NOE	not available	not available	not available

Software:

VNMR v6.1C - collection

xwinnmr v3.5pl6 - collection

AutoAssign v2.2.1 - data analysis

SPARKY v3.110 - data analysis

AutoStruct v2.1.1 - refinement

X-PLOR NIH v2.11.2 - refinement

AGNUS v2.0 - data analysis

PDBStat v4.01 - data analysis

PSVS v1.2 - data analysis

NMR spectrometers:

Bruker AVANCE 600 MHz

BMRB	15476
PDB	2HEP 2JVD 3BHP
DBJ	BAI85472 BAM52440 BAM58016 GAK81555
EMBL	CAB13672 CCU58402 CEI56979 CEJ77403
GB	ADV92690 AEP90959 AFI28488 AFQ57722 AGE63629
REF	NP_389671 WP_003231595 WP_014664125 WP_019714523 WP_024573193
SP	O31818
AlphaFold	O31818