BMRB Entry 7224

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: LACS
BMRB Entry DOI: doi:10.13018/BMR7224
MolProbity Validation Chart

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Title:
Solution NMR structure of Phage-like element PBSX protein xkdW, Northeast Structural Genomics Consortium Target SR355 (CASP Target)
Deposition date:
2006-07-14
Original release date:
2010-03-04
Authors:
Liu, G.; Parish, D.; Xu, D.; Atreya, H.; Sukumaran, D.; Ho, C.; Jiang, M.; Cunningham, K.; Ma, L.-C.; Xiao, R.; Liu, J.; Baran, M.; Swapna, G.; Acton, T.; Rost, B.; Montelione, G.; Szyperski, T.
Citation:

Citation: Liu, G.; Parish, D.; Xu, D.; Atreya, H.; Sukumaran, D.; Ho, C.; Jiang, M.; Cunningham, K.; Ma, L.-C.; Xiao, R.; Liu, J.; Baran, M.; Swapna, G.; Acton, T.; Rost, B.; Montelione, G.; Szyperski, T.. "Solution NMR structure of Phage-like element PBSX protein xkdW, Northeast Structural Genomics Consortium Target SR355"  .

Assembly members:

Assembly members:
Phage-like element PBSX protein xkdW, polymer, 110 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Phage-like element PBSX protein xkdW: MILYDAIMYKYPNAVSRKDF ELRNDGNGSYIEKWNLRAPL PTQAELETWWEELQKNPPYE PPDQVELLAQELSQEKLARK QLEELNKTLGNELSDIKLSL LSLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts216
15N chemical shifts63
1H chemical shifts445

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Phage-like element PBSX protein xkdW1

Entities:

Entity 1, Phage-like element PBSX protein xkdW 110 residues - Formula weight is not available

1   METILELEUTYRASPALAILEMETTYRLYS
2   TYRPROASNALAVALSERARGLYSASPPHE
3   GLULEUARGASNASPGLYASNGLYSERTYR
4   ILEGLULYSTRPASNLEUARGALAPROLEU
5   PROTHRGLNALAGLULEUGLUTHRTRPTRP
6   GLUGLULEUGLNLYSASNPROPROTYRGLU
7   PROPROASPGLNVALGLULEULEUALAGLN
8   GLULEUSERGLNGLULYSLEUALAARGLYS
9   GLNLEUGLUGLULEUASNLYSTHRLEUGLY
10   ASNGLULEUSERASPILELYSLEUSERLEU
11   LEUSERLEUGLUHISHISHISHISHISHIS

Samples:

sample_1: Phage-like element PBSX protein xkdW 1.05 mM; NaN3 0.02%; DTT 100 mM; CaCl2 5 mM; NaCl 100 mM; MES 20 mM; H2O 95%; D2O 5%

sample_cond_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
SIMULTANEOUS HETERONUCLEAR RESOLVED [1H,1H]-NOESYsample_1not availablesample_cond_1
GFT (4,3)D HNNCABCAsample_1not availablesample_cond_1
GFT (4,3)D CABCA(CO)NHNsample_1not availablesample_cond_1
GFT (4,3)D HABCAB(CO)NHNsample_1not availablesample_cond_1
GFT (4,3)D HCCHsample_1not availablesample_cond_1

Software:

VNMR v6.1c - collection

NMRPipe v2.3 - processing

AutoAssign v1.15.1 - data analysis

AutoStruct v2.0 - data analysis

CYANA v21 - structure solution

CNS v1.1 - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB AEP90405
REF WP_014476554 YP_005556379

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks