BMRB Entry 7219

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7219

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Title:
NMR Data for the Human Ubiquitin-Conjugating Enzyme Variant hUev1a
Deposition date:
2006-07-10
Original release date:
2006-10-19
Authors:
Hau, David; Lewis, Michael; Saltibus, Linda; Spyracopoulos, Leo
Citation:

Citation: Hau, David; Lewis, Michael; Saltibus, Linda; Pastushok, Landon; Xiao, Wei; Spyracopoulos, Leo. "Structure and interactions of the ubiquitin-conjugating enzyme variant human Uev1a: implications for enzymatic synthesis of polyubiquitin chains"  Biochemistry 45, 9866-9877 (2006).
PubMed: 16893187

Assembly members:

Assembly members:
hUev1a, polymer, 170 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pGEX-6P

Entity Sequences (FASTA):

Entity Sequences (FASTA):
hUev1a: MPGEVQASYLKSQSKLSDEG RLEPRKFHCKGVKVPRNFRL LEELEEGQKGVGDGTVSWGL EDDEDMTLTRWTGMIIGPPR TIYENRIYSLKIECGPKYPE APPFVRFVTKINMNGVNSSN GVVDPRAISVLAKWQNSYSI KVVLQELRRLMMSKENMKLP QPPEGQCYSN

Data sets:
Data typeCount
13C chemical shifts599
15N chemical shifts170
1H chemical shifts1129
coupling constants56
S2 parameters111
T1 relaxation values248
T2 relaxation values249

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hUev1a1

Entities:

Entity 1, hUev1a 170 residues - Formula weight is not available

1   METPROGLYGLUVALGLNALASERTYRLEU
2   LYSSERGLNSERLYSLEUSERASPGLUGLY
3   ARGLEUGLUPROARGLYSPHEHISCYSLYS
4   GLYVALLYSVALPROARGASNPHEARGLEU
5   LEUGLUGLULEUGLUGLUGLYGLNLYSGLY
6   VALGLYASPGLYTHRVALSERTRPGLYLEU
7   GLUASPASPGLUASPMETTHRLEUTHRARG
8   TRPTHRGLYMETILEILEGLYPROPROARG
9   THRILETYRGLUASNARGILETYRSERLEU
10   LYSILEGLUCYSGLYPROLYSTYRPROGLU
11   ALAPROPROPHEVALARGPHEVALTHRLYS
12   ILEASNMETASNGLYVALASNSERSERASN
13   GLYVALVALASPPROARGALAILESERVAL
14   LEUALALYSTRPGLNASNSERTYRSERILE
15   LYSVALVALLEUGLNGLULEUARGARGLEU
16   METMETSERLYSGLUASNMETLYSLEUPRO
17   GLNPROPROGLUGLYGLNCYSTYRSERASN

Samples:

sample_1: hUev1a, [U-15N; U-13C], 1 mM; NaH2PO4 50 mM; NaCl 250 mM; DTT 0.9 mM; DSS 1.7 mM; D2O 10%

sample_2: hUev1a, [U-15N; U-10% 13C], 0.75 mM; NaH2PO4 50 mM; NaCl 250 mM; DTT 0.9 mM; DSS 1.7 mM; D2O 10%

sample_3: hUev1a, [U-15N], 0.5 mM; NaH2PO4 50 mM; NaCl 250 mM; DTT 0.9 mM; DSS 1.7 mM; D2O 10%

conditions_1: pH: 7.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D_13C-separate_NOESYnot availablenot availableconditions_1
3D_15N-separated_NOESYnot availablenot availableconditions_1
HNHAnot availablenot availableconditions_1
15N HSQCnot availablenot availableconditions_1
HNCACBnot availablenot availableconditions_1
CBCA(CO)NHnot availablenot availableconditions_1
HNCAnot availablenot availableconditions_1
MQ-(H)CCmHm-TOCSYnot availablenot availableconditions_1
H(CC)(CO)NH-TOCSYnot availablenot availableconditions_1
(H)-CC(CO)NH-TOCSYnot availablenot availableconditions_1
HCCH-TOCSYnot availablenot availableconditions_1
HNHBnot availablenot availableconditions_1
(HB)CB(CGCD)HDnot availablenot availableconditions_1
(HB)CB(CGCDCE)HEnot availablenot availableconditions_1
(H)CCH-TROSYnot availablenot availableconditions_1
aromatic 2D 1H 13C HSQCnot availablenot availableconditions_1
2D 1H 13C HSQCnot availablenot availableconditions_1

Software:

VNMR, Varian -

NMRPipe -

SPARKY v3.110 -

NMR spectrometers:

  • Varian Unity INOVA 600 MHz
  • Varian Unity INOVA 500 MHz
  • Varian Unity INOVA 800 MHz

Related Database Links:

PDB
DBJ BAK63082
EMBL CAH92507 CAH93170
GB AAB04629 AAB72015 AAC02755 AAG24229 AAH00468
REF NP_001027459 NP_001030574 NP_001128950 NP_001192006 NP_001192013
SP Q13404 Q3SZ52 Q5R4Z6 Q90879
TPG DAA23316
AlphaFold Q13404 Q3SZ52 Q5R4Z6 Q90879

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks