BMRB Entry 7218

Title:
1H, 13C and 15N assignment of the Master-Rep protein nuclease domain from the Faba Bean Necrotic Yellows Virus
Deposition date:
2006-07-10
Original release date:
2006-10-30
Authors:
Vega-Rocha, Susana; Campos-Olivas, Ramon
Citation:

Citation: Vega-Rocha, Susana; Gronenborn, A.; Gronenborn, B.; Campos-Olivas, Ramon. "(1)H, (13)C, and (15)N NMR assignment of the master Rep protein nuclease domain from the Nanovirus FBNYV"  J. Biomol. NMR 38, 169-169 (2007).
PubMed: 17043745

Assembly members:

Assembly members:
N-terminal DNA binding domain of M-Rep protein from the nanovirus FBNYV, polymer, 138 residues, 15866.0 Da.

Natural source:

Natural source:   Common Name: Faba Bean Necrotic Yellows Virus   Taxonomy ID: 59817   Superkingdom: Viruses   Kingdom: Viridiplantae   Genus/species: Nanovirus Faba Bean Necrotic Yellows Virus

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
N-terminal DNA binding domain of M-Rep protein from the nanovirus FBNYV: GSSHHHHHHSSGLEVLFQGP HMARQVICWCFTLNNPLSPL SLHDSMKYLVYQTEQGEAGN IHFQGYIEMKKRTSLAGMKK LIPGAHFEKRRGTQGEARAY SMKEDTRLEGPWEYGEFVPT IEDKLREVMNDMKITGKR

Data sets:
Data typeCount
13C chemical shifts356
15N chemical shifts127
1H chemical shifts559
coupling constants74

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1monomer1

Entities:

Entity 1, monomer 138 residues - 15866.0 Da.

1   GLYSERSERHISHISHISHISHISHISSER
2   SERGLYLEUGLUVALLEUPHEGLNGLYPRO
3   HISMETALAARGGLNVALILECYSTRPCYS
4   PHETHRLEUASNASNPROLEUSERPROLEU
5   SERLEUHISASPSERMETLYSTYRLEUVAL
6   TYRGLNTHRGLUGLNGLYGLUALAGLYASN
7   ILEHISPHEGLNGLYTYRILEGLUMETLYS
8   LYSARGTHRSERLEUALAGLYMETLYSLYS
9   LEUILEPROGLYALAHISPHEGLULYSARG
10   ARGGLYTHRGLNGLYGLUALAARGALATYR
11   SERMETLYSGLUASPTHRARGLEUGLUGLY
12   PROTRPGLUTYRGLYGLUPHEVALPROTHR
13   ILEGLUASPLYSLEUARGGLUVALMETASN
14   ASPMETLYSILETHRGLYLYSARG

Samples:

sample_1: FBNYV M-Rep (1-117), [U-10% 13C; U-15N], 0.4; Sodium phosphate 20; NaCl 600; DTT 1; D2O 8%

sample_2: FBNYV M-Rep (1-117), [U-13C; U-15N], 0.4; Sodium phosphate 20; NaCl 600; DTT 1; D2O 8%

conditions_1: ionic strength: 1.264 M; pH: 6.6; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H15N_HSQCnot availablenot availableconditions_1
3D HNCOnot availablenot availableconditions_1
3D HNCACBnot availablenot availableconditions_1
3D CBCA (CO) NHnot availablenot availableconditions_1
3D HNHAnot availablenot availableconditions_1
3D 15N edited NOESYnot availablenot availableconditions_1
3D CC (CO) NHnot availablenot availableconditions_1
3D 15N edited TOCSYnot availablenot availableconditions_1

Software:

NMRPipe v97.027.12.56 -

NMRView v5.0.20 -

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

BMRB 7112
PDB
EMBL CAA72209 CAB44020
GB ACX50503
REF NP_619567
SP O39828 Q9WIJ5
AlphaFold O39828 Q9WIJ5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks