BMRB Entry 7210

Name: plant homeodomain finger of the tumour suppressor ING4
Published: 2007-01-09

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7210

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

plant homeodomain finger of the tumour suppressor ING4

Deposition date:

2006-07-04

Original release date:

2007-01-09

Authors:

Palacios, Alicia; Garcia, Pascal; Padro, Daniel; Lopez-Hernandez, Eva; Blanco, Francisco Jose

Citation:

Citation: Palacios, Alicia; Garcia, Pascal; Padro, Daniel; Lopez-Hernandez, Eva; Martin, I.; Blanco, Francisco Jose. "Solution structure and NMR characterization of the binding to methylated histone tails of the plant homeodomain finger of the tumour suppressor ING4." FEBS Lett. 580, 6903-6908 (2006).
PubMed: 17157298

Assembly members:

Assembly members:
ING4_PHD, polymer, 63 residues, 7315 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: not available Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Vector: pET11d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ING4_PHD: MDMPVDPNEPTYCLCHQVSY GEMIGCDNPDCSIEWFHFAC VGLTTKPRGKWFCPRCSQER KKK

Data sets:

assigned_chemical_shifts
- chem_shift_list_1
coupling_constants
- J_coupling_list_1

Data type	Count
13C chemical shifts	256
15N chemical shifts	56
1H chemical shifts	403
coupling constants	49

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Plant HomeoDomain of ING4	1
2	ZINC (II) ION, 1	2
3	ZINC (II) ION, 2	2

Entities:

Entity 1, Plant HomeoDomain of ING4 63 residues - 7315 Da.

1

MET

ASP

MET

PRO

VAL

ASP

PRO

ASN

GLU

PRO

2

THR

TYR

CYS

LEU

CYS

HIS

GLN

VAL

SER

TYR

3

GLY

GLU

MET

ILE

GLY

CYS

ASP

ASN

PRO

ASP

4

CYS

SER

ILE

GLU

TRP

PHE

HIS

PHE

ALA

CYS

5

VAL

GLY

LEU

THR

LYS

PRO

ARG

GLY

LYS

6

TRP

PHE

CYS

PRO

ARG

CYS

SER

GLN

GLU

ARG

7

LYS

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: ING4_PHD, [U-95% 13C; U-90% 15N], 1.2 mM; sodium phosphate 20 mM; NaCl 50 mM; DTT 1 mM; D2O 9%

sample_2: ING4_PHD, [U-95% 13C; U-90% 15N], 1.2 mM; sodium phosphate 20 mM; NaCl 50 mM; DTT 1 mM; D2O 100%

sample_3: ING4_PHD, [U-90% 15N], 0.9 mM; sodium phosphate 20 mM; NaCl 50 mM; DTT 1 mM; D2O 9%

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H15N_HSQC	not available	not available	conditions_1
2D 1H13C_HSQC	not available	not available	conditions_1
3D CBCA(CO)NH	not available	not available	conditions_1
3D HNCACB	not available	not available	conditions_1
3D (H)C(CCO)NH	not available	not available	conditions_1
3D HNHA	not available	not available	conditions_1
3D HNHB	not available	not available	conditions_1
3D 15N edited TOCSY	not available	not available	conditions_1
3D 15N edited NOESY	not available	not available	conditions_1
3D 13C edited NOESY	not available	not available	conditions_1
3D HCCH-TOCSY	not available	not available	conditions_1
3D HNCO	not available	not available	conditions_1

Software:

NMRView v5.0.20, One Moon Scientific Inc -

NMRPipe v97.027.12.56, . -

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks