BMRB Entry 7206
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7206
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Citation: Huang, Yu-Chie; Lin, Yu-Min; Chang, Tin-Wei; Wu, Shi-Jung; Lee, Yan-Shin; Yu, Hui-Ming; Chang, Dah-Tsyr; Chen, Chinpan; Wu, Shih-Hsiung; Liao, You-Di. "The flexible and clustered lysine residues of human ribonuclease 7 are critical
for membrane permeability and antimicrobial activity" J. Biol. Chem. 282, 4626-4633 (2007).
PubMed: 17150966
Assembly members:
human RNase 7, polymer, 129 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
human RNase 7: MKPKGMTSSQWFKIQHMQPS
PQACNSAMKNINKHTKRCKD
LNTFLHEPFSSVAATCQTPK
IACKNGDKNCHQSHGPVSLT
MCKLTSGKYPNCRYKEKRQN
KSYVVACKPPQKKDSQQFHL
VPVHLDRVL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 522 |
| 15N chemical shifts | 118 |
| 1H chemical shifts | 849 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | human RNase 7 | 1 |
Entities:
Entity 1, human RNase 7 129 residues - Formula weight is not available
| 1 | MET | LYS | PRO | LYS | GLY | MET | THR | SER | SER | GLN | ||||
| 2 | TRP | PHE | LYS | ILE | GLN | HIS | MET | GLN | PRO | SER | ||||
| 3 | PRO | GLN | ALA | CYS | ASN | SER | ALA | MET | LYS | ASN | ||||
| 4 | ILE | ASN | LYS | HIS | THR | LYS | ARG | CYS | LYS | ASP | ||||
| 5 | LEU | ASN | THR | PHE | LEU | HIS | GLU | PRO | PHE | SER | ||||
| 6 | SER | VAL | ALA | ALA | THR | CYS | GLN | THR | PRO | LYS | ||||
| 7 | ILE | ALA | CYS | LYS | ASN | GLY | ASP | LYS | ASN | CYS | ||||
| 8 | HIS | GLN | SER | HIS | GLY | PRO | VAL | SER | LEU | THR | ||||
| 9 | MET | CYS | LYS | LEU | THR | SER | GLY | LYS | TYR | PRO | ||||
| 10 | ASN | CYS | ARG | TYR | LYS | GLU | LYS | ARG | GLN | ASN | ||||
| 11 | LYS | SER | TYR | VAL | VAL | ALA | CYS | LYS | PRO | PRO | ||||
| 12 | GLN | LYS | LYS | ASP | SER | GLN | GLN | PHE | HIS | LEU | ||||
| 13 | VAL | PRO | VAL | HIS | LEU | ASP | ARG | VAL | LEU |
Samples:
sample_1: human RNase 7, [U-95% 13C; U-90% 15N], 0.6 mM
conditions_1: pH: 3.5; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D TOCSY | sample_1 | not available | conditions_1 |
| 2D NOESY | sample_1 | not available | conditions_1 |
| 2D 1H-13C HSQC | sample_1 | not available | conditions_1 |
| 2D 1H-15N HSQC | sample_1 | not available | conditions_1 |
| 3D HNCACB | sample_1 | not available | conditions_1 |
| 3D CBCA(CO)NH | sample_1 | not available | conditions_1 |
| 3D HNCO | sample_1 | not available | conditions_1 |
| 3D HN(CA)CO | sample_1 | not available | conditions_1 |
| 3D 15N NOESY-HSQC | sample_1 | not available | conditions_1 |
| 3D 15N TOCSY-HSQC | sample_1 | not available | conditions_1 |
| 3D C(CO)NH | sample_1 | not available | conditions_1 |
| 3D HC(CO)NH | sample_1 | not available | conditions_1 |
| 3D HCCH-TOCSY | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- BRUKER DRX 600 MHz
- BRUKER AVANCE 800 MHz
Related Database Links:
| PDB | |
| DBJ | BAF85386 |
| EMBL | CAC20410 CAC84457 CAC84458 CAC84462 |
| GB | AAH74960 AAI12335 AAO12510 AAQ89455 AIC57364 |
| REF | NP_115961 XP_003314288 XP_003804787 XP_004054925 |
| SP | Q9H1E1 |
| TPE | CDG31915 CDG31930 CDG31937 |
| AlphaFold | Q9H1E1 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks