BMRB Entry 7202

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7202

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for dSUMO
Deposition date:
2006-06-30
Original release date:
2007-07-02
Authors:
Kumar, Ashutosh; Misra, Jyoti; Mittal, Rohit; Hosur, Ramakrishna
Citation:

Citation: Kumar, Dinesh; Kumar, Ashutosh; Misra, Jyoti; Chugh, Jeetender; Sharma, Shilpy; Hosur, Ramakrishna. "1H, 15N, 13C resonance assignment of folded and 8 M urea-denatured state of SUMO from Drosophila melanogaster"  Biomol. NMR Assignments 2, 13-15 (2008).

Assembly members:

Assembly members:
dSUMO, polymer, 90 residues, 9972.2 Da.

Natural source:

Natural source:   Common Name: Drosophila melanogaster   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dSUMO: GSMSDEKKGGETEHINLKVL GQDNAVVQFKIKKHTPLRKL MNAYCDRAGLSMQVVRFRFD GQPINENDTPTSLEMEEGDT IEVYQQQTGG

Data sets:
Data typeCount
13C chemical shifts356
15N chemical shifts90
1H chemical shifts564

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dSUMO1

Entities:

Entity 1, dSUMO 90 residues - 9972.2 Da.

1   GLYSERMETSERASPGLULYSLYSGLYGLY
2   GLUTHRGLUHISILEASNLEULYSVALLEU
3   GLYGLNASPASNALAVALVALGLNPHELYS
4   ILELYSLYSHISTHRPROLEUARGLYSLEU
5   METASNALATYRCYSASPARGALAGLYLEU
6   SERMETGLNVALVALARGPHEARGPHEASP
7   GLYGLNPROILEASNGLUASNASPTHRPRO
8   THRSERLEUGLUMETGLUGLUGLYASPTHR
9   ILEGLUVALTYRGLNGLNGLNTHRGLYGLY

Samples:

sample_1: dSUMO, [U-13C; U-15N], 1 ± 0.1 mM; Sodium Phosphate 100 mM

conditions_1: pH: 5.6; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
HNNsample_1not availableconditions_1
HN(C)Nsample_1not availableconditions_1
CBCACONHsample_1not availableconditions_1
CBCANHsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
TOCSY-HSQCsample_1not availableconditions_1
15N-1H HSQCsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

BMRB 15473
PDB
EMBL CBA18485
GB AAD19219 AAF31702 AAF52470 AAL28638 EAL32843
REF NP_477411 XP_001355784 XP_001650444 XP_001962391 XP_001970104

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks