BMRB Entry 7194

Title:
Transcription Factor IIIA zinc fingers 4-6 bound to 5S rRNA 55mer   PubMed: 16405997
Deposition date:
2006-06-27
Original release date:
2006-08-12
Authors:
Lee, Brian
Citation:

Citation: Lee, Brian; Xu, Jing; Clarkson, Bryan; Martinez-Yamout, Maria; Dyson, H.; Case, Dave; Gottesfeld, Joel; Wright, Peter. "Induced Fit and 'Lock and Key' Recognition of 5 S RNA by Zinc Fingers of Transcription Factor IIIA"  J. Mol. Biol. 357, 275-291 (2006).

Assembly members:

Assembly members:
5S rRNA 55mer, polymer, 55 residues, Formula weight is not available
TFIIIA zf46, polymer, 87 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: African clawed frog   Taxonomy ID: 8355   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus laevis

Experimental source:   Production method: enzymatic semisynthesis

Experimental source:

Natural source:   Common Name: African clawed frog   Taxonomy ID: 8355   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus laevis

Experimental source:   Production method: enzymatic semisynthesis

Data sets:
Data typeCount
13C chemical shifts297
15N chemical shifts97
1H chemical shifts583
residual dipolar couplings98

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
15S rRNA1
2TFIIIA zf462
3ZINC (II) ION, 13
4ZINC (II) ION, 23
5ZINC (II) ION, 33

Entities:

Entity 1, 5S rRNA 55 residues - Formula weight is not available

1   GGGCCACACC
2   UCUUGGGCCU
3   GGUUAGUACC
4   UGUUCGCUGG
5   GAAUACCAGG
6   UGCCC

Entity 2, TFIIIA zf46 87 residues - Formula weight is not available

1   METTYRVALCYSHISPHEGLUASNCYSGLY
2   LYSALAPHELYSLYSHISASNGLNLEULYS
3   VALHISGLNPHESERHISTHRGLNGLNLEU
4   PROTYRGLUCYSPROHISGLUGLYCYSASP
5   LYSARGPHESERLEUPROSERARGLEULYS
6   ARGHISGLULYSVALHISALAGLYTYRPRO
7   CYSLYSLYSASPASPSERCYSSERPHEVAL
8   GLYLYSTHRTRPTHRLEUTYRLEULYSHIS
9   VALALAGLUCYSHISGLNASP

Entity 3, ZINC (II) ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: 5S rRNA 55mer0.6 – 2.0 mM; TFIIIA zf46, [U-15N], 0.6 – 2.0 mM; ZINC (II) ION1.8 – 6.0 mM; KH2PO4 10 mM; DTT 20 mM

sample_2: 5S rRNA 55mer0.6 – 2.0 mM; TFIIIA zf46, [U-13; U-15N], 0.6 – 2.0 mM; ZINC (II) ION1.8 – 6.0 mM; KH2PO4 10 mM; DTT 20 mM

sample_3: 5S rRNA 55mer0.6 – 2.0 mM; TFIIIA zf46, [U-2H; U-13; U-15N], 0.6 – 2.0 mM; ZINC (II) ION1.8 – 6.0 mM; KH2PO4 10 mM; DTT 20 mM

sample_4: 5S rRNA 55mer, [U-15N], 0.6 – 2.0 mM; TFIIIA zf46, [U-15N], 0.6 – 2.0 mM; ZINC (II) ION1.8 – 6.0 mM; KH2PO4 10 mM; DTT 20 mM

sample_5: 5S rRNA 55mer, [U-15N], 0.6 – 2.0 mM; TFIIIA zf460.6 – 2.0 mM; ZINC (II) ION1.8 – 6.0 mM; KH2PO4 10 mM; DTT 20 mM

sample_6: 5S rRNA 55mer, [U-13C; U-15N], 0.6 – 2.0 mM; TFIIIA zf460.6 – 2.0 mM; ZINC (II) ION1.8 – 6.0 mM; KH2PO4 10 mM; DTT 20 mM

conditions_1: ionic strength: 10 mM; pH: 6.5; temperature: 291 K

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availableconditions_1
HNCAnot availablenot availableconditions_1
(HCA)CO(CA)NHnot availablenot availableconditions_1
CBCA(CO)NHnot availablenot availableconditions_1
HCACOnot availablenot availableconditions_1
13C-HSQCnot availablenot availableconditions_1
(H)C(CO)NH-TOCSYnot availablenot availableconditions_1
(H)CCH-COSYnot availablenot availableconditions_1
HCCH-TOCSYnot availablenot availableconditions_1
(HB)CB(CGCD)HDnot availablenot availableconditions_1
TROSY based HNCOnot availablenot availableconditions_1
TROSY based HNCAnot availablenot availableconditions_1
TROSY based HN(CA)COnot availablenot availableconditions_1
3D 15N NOESY HSQCnot availablenot availableconditions_1
3D shared-time 15N/13C NOESY HSQCnot availablenot availableconditions_1
3D 13C HMQC-NOESYnot availablenot availableconditions_1
15N HMQCnot availablenot availableconditions_1
water flip-back 2D NOESYnot availablenot availableconditions_1
TROSY based HNN-COSYnot availablenot availableconditions_1
13C HMQCnot availablenot availableconditions_1
2JHN 15N HMQCnot availablenot availableconditions_1
3D 13C HMQC NOESYnot availablenot availableconditions_1
2D double half filtered NOESYnot availablenot availableconditions_1
3D 13C edit/filter NOESYnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 800 MHz
  • Bruker DMX 750 MHz
  • Bruker DRX 600 MHz
  • Bruker AMX-II 500 MHz
  • Bruker AMX 500 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts