BMRB Entry 7193

Title:
Sequence specific resonance assignments of a hypothetical protein PA1123 (NESG ID: PaT4) from Pseudomonas aeruginosa
Deposition date:
2006-06-27
Original release date:
2006-08-11
Authors:
Srisailam, Sampath; Lemak, Alexander; Yee, Adelinda; Lukin, Jonathan; Arrowsmith, Cheryl
Citation:

Citation: Srisailam, Sampath; Lemak, Alexander; Yee, Adelinda; Lukin, Jonathan; Arrowsmith, Cheryl. "Sequence specific assignment of a hypothetical protein PA1123 (NESG ID: PaT4) from Pseudomonas aeruginosa"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
hypothetical protein, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts434
15N chemical shifts108
1H chemical shifts714

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PA11231

Entities:

Entity 1, PA1123 106 residues - Formula weight is not available

1   METSERILETHRSERTHRASPILECYSGLN
2   ALAALAASPALALEULYSGLYPHEVALGLY
3   PHEASNARGLYSTHRGLYARGTYRILEVAL
4   ARGPHESERGLUASPSERPHEGLYMETASP
5   VALALAASPASPSERILETHRPROTHRSER
6   GLUPHEVALTRPSERSERVALARGASPASP
7   VALMETARGLEUGLYARGGLUGLNLEUGLN
8   ILELEULEUGLUGLNASNILEASNGLUARG
9   LEUASNILEGLYGLUPROLEULEUVALTYR
10   LEUARGARGGLNASPLEUPROGLUILETHR
11   ALAGLNARGGLNLEUARG

Samples:

sample_1: hypothetical protein, [U-15N; U-13C], 1 ± 0.1 mM; Buffer 10 ± 0.05 mM; Salt 400 ± 0.05 mM; DTT 10 ± 0.05 mM; NaN3 0.01 ± 0.001 %; Benzamidine 1 ± 0.001 mM

conditions_1: pH: 6.5; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
1H13C-HSQCsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
CCTOCSY NHsample_1not availableconditions_1
HCCTOCSY NHsample_1not availableconditions_1
HC(C)H-TOCSYsample_1not availableconditions_1
HCC(H)-TOCSYsample_1not availableconditions_1
15N-NOESYsample_1not availableconditions_1
13C-NOESYsample_1not availableconditions_1

Software:

SPARKY v3.95 -

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAK88853 BAP20595 BAP52175 GAA16659 GAJ52478
EMBL CAW28951 CCQ87904 CDH72324 CDH78589 CDI89265
GB AAG04512 ABJ10291 ABR86109 AEO76390 AFM66282
REF NP_249814 WP_003082265 WP_003157837 WP_003163119 WP_023129052

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks