BMRB Entry 7191
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR7191
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Citation: Eletsky, Alexander; Atreya, Hanudatta; Liu, Gaohua; Sukumaran, Dinesh; Garcia, Maite; Yee, Adelinda; Arrowsmith, Cheryl; Szyperski, Thomas. "Solution NMR Structure of Pseudomonas aeruginosa Hypothetical Protein RPA1041" Proteins: Struct. Funct. Genet. ., .-..
Assembly members:
pat90, polymer, 97 residues, Formula weight is not available
Natural source: Common Name: P. aeruginosa Taxonomy ID: 287 Superkingdom: Eubacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa
Experimental source: Production method: recombinant technology Vector: p15Tv lic
Entity Sequences (FASTA):
pat90: MGSSHHHHHHSSGRENLYFQ
GHLRELLRTNDAVLLSAVGA
LLDGADIGHLVLDQNMSILE
GSLGVIPRRVLVHEDDLAGA
RRLLTDAGLAHELRSDD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 337 |
| 15N chemical shifts | 86 |
| 1H chemical shifts | 570 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | pat90 monomer | 1 |
Entities:
Entity 1, pat90 monomer 97 residues - Formula weight is not available
Contains 22-residue N-terminal His-tag MGSSHHHHHHSSGRENLYFQGH
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | ARG | GLU | ASN | LEU | TYR | PHE | GLN | ||||
| 3 | GLY | HIS | LEU | ARG | GLU | LEU | LEU | ARG | THR | ASN | ||||
| 4 | ASP | ALA | VAL | LEU | LEU | SER | ALA | VAL | GLY | ALA | ||||
| 5 | LEU | LEU | ASP | GLY | ALA | ASP | ILE | GLY | HIS | LEU | ||||
| 6 | VAL | LEU | ASP | GLN | ASN | MET | SER | ILE | LEU | GLU | ||||
| 7 | GLY | SER | LEU | GLY | VAL | ILE | PRO | ARG | ARG | VAL | ||||
| 8 | LEU | VAL | HIS | GLU | ASP | ASP | LEU | ALA | GLY | ALA | ||||
| 9 | ARG | ARG | LEU | LEU | THR | ASP | ALA | GLY | LEU | ALA | ||||
| 10 | HIS | GLU | LEU | ARG | SER | ASP | ASP |
Samples:
NC: pat90, [U-100% 13C; U-100% 15N], 0.5 mM; MOPS 10 mM; NaCl 450 mM; NaN3 0.01 % w/w; ZnSO4 10 mM; DTT 10 mM; benzamidine 1 mM; inhibitor cocktail 1
NC7: pat90, [U-7% 13C; U-100% 15N], 0.5 mM; MOPS 10 mM; NaCl 450 mM; NaN3 0.01 % w/w; ZnSO4 10 mM; DTT 10 mM; benzamidine 1 mM; inhibitor cocktail 1
conditions_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H15N_HSQC | NC | isotropic | conditions_1 |
| 2D 1H13C_HSQC (ali) | NC | isotropic | conditions_1 |
| 2D 1H13C_HSQC (aro) | NC | isotropic | conditions_1 |
| 2D CT-1H13C_HSQC (ali) | NC | isotropic | conditions_1 |
| 2D CT-1H13C_HSQC (aro) | not available | not available | conditions_1 |
| 3D HNCO | NC | isotropic | conditions_1 |
| (4,3)D GFT CABCA(CO)NHN | NC | isotropic | conditions_1 |
| 3D HAB(CO)NH | NC | isotropic | conditions_1 |
| (4,3)D GFT HNNCABCA | NC | isotropic | conditions_1 |
| (4,3)D GFT HCCH-COSY (ali) | NC | isotropic | conditions_1 |
| 3D (H)CCH-COSY (ali) | NC | isotropic | conditions_1 |
| 3D 15N-, 13Cali-, 13Caro-resolved NOESY | NC | isotropic | conditions_1 |
| 2D 28ms CT-1H13C_HSQC (methyl) | not available | not available | conditions_1 |
| 2D 56ms CT-1H13C_HSQC (methyl) | not available | not available | conditions_1 |
| 3D (H)CCH-TOCSY (ali) | NC | isotropic | conditions_1 |
Software:
VNMR v6.1C, Varian, Inc. - NMR data acqusition
XEASY v1.3.13, Bartels et al. - Spectral analysis
CARA v1.5.3, Keller and Wuthrich - Spectral analysis
PROSA v6.0, Guntert - NMR data processing
NMR spectrometers:
- Varian, Inc INOVA 600 MHz
- Varian, Inc INOVA 750 MHz
Related Database Links:
| PDB | |
| EMBL | CAE26484 |
| GB | ACE99772 |
| REF | NP_946392 WP_011156574 WP_012494775 YP_001990248 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks