BMRB Entry 7189

Title:
NMR assignments of the low molecular weight protein tyrosine phosphatase from Campylobacter Jejuni   PubMed: 17008719
Deposition date:
2006-06-22
Original release date:
2006-10-12
Authors:
Tolkatchev, Dmitri; Shaykhutdinov, Rustem; Xu, Ping; Ni, Feng
Citation:

Citation: Tolkatchev, Dmitri; Shaykhutdinov, Rustem; Xu, Ping; Plamondon, Josee; Watson, David; Young, N.; Ni, Feng. "Three-dimensional structure and ligand interactions of the low molecular weight protein tyrosine phosphatase from Campylobacter jejuni"  Protein Sci. 15, 2381-2394 (2006).

Assembly members:

Assembly members:
LMW PTPase, polymer, 156 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Campylobacter jejuni   Taxonomy ID: 197   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Campylobacter C. jejuni

Experimental source:   Production method: recombinant technology   Vector: PCWORI+

Experimental source:

Natural source:   Common Name: Campylobacter jejuni   Taxonomy ID: 197   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Campylobacter C. jejuni

Experimental source:   Production method: recombinant technology   Vector: PCWORI+

Data sets:
Data typeCount
13C chemical shifts1087
15N chemical shifts300
1H chemical shifts1609

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cj12581

Entities:

Entity 1, Cj1258 156 residues - Formula weight is not available

1   METLYSLYSILELEUPHEILECYSLEUGLY
2   ASNILECYSARGSERPROMETALAGLUPHE
3   ILEMETLYSASPLEUVALLYSLYSALAASN
4   LEUGLULYSGLUPHEPHEILEASNSERALA
5   GLYTHRSERGLYGLUHISASPGLYGLUGLY
6   METHISTYRGLYTHRLYSASNLYSLEUALA
7   GLNLEUASNILEGLUHISLYSASNPHETHR
8   SERLYSLYSLEUTHRGLNLYSLEUCYSASP
9   GLUSERASPPHELEUILETHRMETASPASN
10   SERASNPHELYSASNVALLEULYSASNPHE
11   THRASNTHRGLNASNLYSVALLEULYSILE
12   THRASPPHESERPROSERLEUASNTYRASP
13   GLUVALPROASPPROTRPTYRSERGLYASN
14   PHEASPGLUTHRTYRLYSILELEUSERLEU
15   ALACYSLYSASNLEULEUVALPHELEUSER
16   LYSHISHISHISHISHIS

Samples:

sample_1: Cj1258, [U-15N; U-13C], 0.7; sodium phosphate 20 mM; EDTA 0.2 mM; NaN3 0.01%; water 90%; deuterated water, [U-99.96% 2H], 10%

conditions_1: pH: 5.8; temperature: 298 K

conditions_2: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HBHAcoNHsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
CBCAcoNHsample_1not availableconditions_1
H(C)CH-COSYsample_1not availableconditions_1
15N-edited_NOESY-HSQCsample_1not availablenot available
13C-edited_NOESY-HSQCsample_1not availablenot available
aromatic_13C-edited_NOESY-HSQCsample_1not availablenot available
15N,1H-HSQCsample_1not availableconditions_1
13C,1H-HSQCsample_1not availableconditions_1
15N,1H-HSQC_2sample_1not availableconditions_2
HBHAcoNH_2sample_1not availableconditions_2
HNCACB_2sample_1not availableconditions_2
HNCO_2sample_1not availableconditions_2
CBCAcoNH_2sample_1not availableconditions_2

Software:

NMRPipe v1.7 - Data processing

NMRView v5.0.4 - Data analysis, Data visualization

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

PDB
EMBL CAL35373 CCK67350 CDH63092
GB AAW35713 ABS43351 ABV52801 ADC28835 ADN91432
REF WP_002853170 WP_002853721 WP_002856382 WP_002858269 WP_002860467

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks