BMRB Entry 7186

Name: Backbone 1H Chemical Shift Assignments for Magi 5
Published: 2008-07-16

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PDB ID: 2gx1
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7186
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H Chemical Shift Assignments for Magi 5

Deposition date:

2006-06-21

Original release date:

2008-07-16

Authors:

Sabo, Jennifer; Corzo, Gerardo; Bosmans, Frank; Billen, Bert; Villegas, Elba; Tygat, Jan; Norton, Raymond

Citation:

Citation: Corzo, Gerardo; Sabo, Jennifer; Bosmans, Frank; Billen, Bert; Villegas, Elba; Tygat, Jan; Norton, Raymond. "Solution structure and alanine scan of a spider toxin that affects the activation of mammalian voltage-gated sodium channels" J. Biol. Chem. 282, 4643-4652 (2007).
PubMed: 17148449

Assembly members:

Assembly members:
Magi 5, polymer, 29 residues, 3268.8 Da.

Natural source:

Natural source: Common Name: Macrothele gigas Taxonomy ID: 223896 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Macrothele gigas

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Magi 5: GCKLTFWKCKNKKECCGWNA CALGICMPR

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1
coupling_constants
- J_values_set_1

Data type	Count
1H chemical shifts	178
coupling constants	14

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Magi 5 polypeptide	1

Entities:

Entity 1, Magi 5 polypeptide 29 residues - 3268.8 Da.

1

GLY

CYS

LYS

LEU

THR

PHE

TRP

LYS

CYS

LYS

2

ASN

LYS

GLU

CYS

GLY

TRP

ASN

ALA

3

CYS

ALA

LEU

GLY

ILE

CYS

MET

PRO

ARG

Samples:

sample_1: Magi 5 2.2 mM; H2O 95%; D2O 5%

sample_cond_1: pH: 4.5; pressure: 1 atm; temperature: 278 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	not available	sample_cond_1
2D 1H-1H NOESY	sample_1	not available	sample_cond_1
2D 1H-1H DQF-COSY	sample_1	not available	sample_cond_1

Software:

xwinnmr v3.5 - processing

TOPSPIN v1.3 - processing

XEASY v1.3.13 - data analysis

CYANA v1.0.6 - structure solution

X-PLOR NIH v2.9.3 - refinement

NMR spectrometers:

Bruker DRX 600 MHz
Bruker Avance 800 MHz