BMRB Entry 7185
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7185
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Yang, F.; Vought, B.; Satterlee, J.; Walker, A.; Jim_Sun, Z.; Watts, J.; DeBeaumont, R.; Saito, R.; Hyberts, S.; Yang, S.; Macol, C.; Iyer, L.; Tjian, R.; van den Heuvel, S.; Hart, A.; Wagner, G.; Naar, A.. "An ARC/Mediator subunit required for SREBP control of cholesterol and lipid homeostasis" Nature 442, 700-704 (2006).
PubMed: 16799563
Assembly members:
Positive cofactor 2 glutamine/Q-rich-associated protein, polymer, 77 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Positive cofactor 2 glutamine/Q-rich-associated protein: GAMGQETDWRSTAFRQKLVS
QIEDAMRKAGVAHSKSSKDM
ESHVFLKAKTRDEYLSLVAR
LIIHFRDIHNKKSQASV
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 254 |
| 15N chemical shifts | 75 |
| 1H chemical shifts | 388 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Positive cofactor 2 glutamine/Q-rich-associated protein | 1 |
Entities:
Entity 1, Positive cofactor 2 glutamine/Q-rich-associated protein 77 residues - Formula weight is not available
| 1 | GLY | ALA | MET | GLY | GLN | GLU | THR | ASP | TRP | ARG | ||||
| 2 | SER | THR | ALA | PHE | ARG | GLN | LYS | LEU | VAL | SER | ||||
| 3 | GLN | ILE | GLU | ASP | ALA | MET | ARG | LYS | ALA | GLY | ||||
| 4 | VAL | ALA | HIS | SER | LYS | SER | SER | LYS | ASP | MET | ||||
| 5 | GLU | SER | HIS | VAL | PHE | LEU | LYS | ALA | LYS | THR | ||||
| 6 | ARG | ASP | GLU | TYR | LEU | SER | LEU | VAL | ALA | ARG | ||||
| 7 | LEU | ILE | ILE | HIS | PHE | ARG | ASP | ILE | HIS | ASN | ||||
| 8 | LYS | LYS | SER | GLN | ALA | SER | VAL |
Samples:
sample_1: Positive cofactor 2 glutamine/Q-rich-associated protein, [U-15N; U-13C], 1 mM; NaCl 30 mM; phosphate buffer 10 mM; H2O 90%; D2O 10%
sample_cond_1: ionic strength: 30 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | sample_1 | not available | sample_cond_1 |
| HNCOCA | sample_1 | not available | sample_cond_1 |
| HNCACB | sample_1 | not available | sample_cond_1 |
| HNCOCACB | sample_1 | not available | sample_cond_1 |
| HNCO | sample_1 | not available | sample_cond_1 |
| HNCACO | sample_1 | not available | sample_cond_1 |
| HN(COCA)NH | sample_1 | not available | sample_cond_1 |
| N15noesy | sample_1 | not available | sample_cond_1 |
| tocsy | sample_1 | not available | sample_cond_1 |
| CCONH | sample_1 | not available | sample_cond_1 |
| HCCONH | sample_1 | not available | sample_cond_1 |
| aliphatic | sample_1 | not available | sample_cond_1 |
| aromatic-C13noesy | sample_1 | not available | sample_cond_1 |
| HCCHTOCSY | sample_1 | not available | sample_cond_1 |
Software:
PROSA v3.7 - processing
CARA v1.5 - data analysis
CYANA v2.1 - refinement, structure solution
NMR spectrometers:
- Bruker AVANCE 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAC03446 BAD21436 BAE38826 BAE41448 BAG57747 |
| EMBL | CAG30423 CAK54568 CAK54867 |
| GB | AAC12944 AAH03078 AAH13985 AAH54779 AAI42513 |
| REF | NP_001003891 NP_001035773 NP_001092645 NP_001101795 NP_001267180 |
| SP | Q924H2 Q96RN5 |
| TPG | DAA20413 |
| AlphaFold | Q924H2 Q96RN5 |