BMRB Entry 7180

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR7180
MolProbity Validation Chart

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Title:
NMR structure of UPF0301 PROTEIN SO3346 from Shewanella oneidensis: Northeast Structural Genomics Consortium target SOR39
Deposition date:
2006-06-21
Original release date:
2007-11-21
Authors:
Singarapu, K.; Liu, G.; Eletsky, A.; Xu, D.; Sukumaran, D.; Mei, J.; Xiao, R.; Cunningham, K.; Ma, L.; Ritu, S.; Acton, T.; Rost, B.; Montelione, G.; Szyperski, T.
Citation:

Citation: Singarapu, K.; Liu, G.; Eletsky, A.; Xu, D.; Sukumaran, D.; Mei, J.; Xiao, R.; Cunningham, K.; Ma, L.; Ritu, S.; Acton, T.; Rost, B.; Montelione, G.; Szyperski, T.. "NMR structure of UPF0301 PROTEIN SO3346 from Shewanella oneidensis: Northeast Structural Genomics Consortium target SOR39"  .

Assembly members:

Assembly members:
UPF0301 protein SO3346, polymer, 195 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Shewanella oneidensis   Taxonomy ID: 70863   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Shewanella oneidensis

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UPF0301 protein SO3346: MESLQNHFLIAMPSLDDTFF ERTVIYLCEHDEKGAMGLVI NKPLGIEVNSLLEQMDLPTE QVSADLAMGSQVLMGGPVSQ DRGFVLHTSQPYWANSTELG SGLMLTTSRDVLTAIGSKRS PDKFLVALGYAGWSKNQLEQ ELADNSWLTIPADHALLFDI NHEDRWQQASRSLGFEAWQL STQAGHALEHHHHHH

Data sets:
Data typeCount
13C chemical shifts503
15N chemical shifts135
1H chemical shifts1029

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UPF0301 protein SO33461

Entities:

Entity 1, UPF0301 protein SO3346 195 residues - Formula weight is not available

1   METGLUSERLEUGLNASNHISPHELEUILE
2   ALAMETPROSERLEUASPASPTHRPHEPHE
3   GLUARGTHRVALILETYRLEUCYSGLUHIS
4   ASPGLULYSGLYALAMETGLYLEUVALILE
5   ASNLYSPROLEUGLYILEGLUVALASNSER
6   LEULEUGLUGLNMETASPLEUPROTHRGLU
7   GLNVALSERALAASPLEUALAMETGLYSER
8   GLNVALLEUMETGLYGLYPROVALSERGLN
9   ASPARGGLYPHEVALLEUHISTHRSERGLN
10   PROTYRTRPALAASNSERTHRGLULEUGLY
11   SERGLYLEUMETLEUTHRTHRSERARGASP
12   VALLEUTHRALAILEGLYSERLYSARGSER
13   PROASPLYSPHELEUVALALALEUGLYTYR
14   ALAGLYTRPSERLYSASNGLNLEUGLUGLN
15   GLULEUALAASPASNSERTRPLEUTHRILE
16   PROALAASPHISALALEULEUPHEASPILE
17   ASNHISGLUASPARGTRPGLNGLNALASER
18   ARGSERLEUGLYPHEGLUALATRPGLNLEU
19   SERTHRGLNALAGLYHISALALEUGLUHIS
20   HISHISHISHISHIS

Samples:

sample_1: UPF0301 protein SO3346; Arginine 50 mM; DTT 10 mM; Bis Tris 50 mM; H2O 95%; D2O 5%

sample_cond_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
[4,3]D GFTsample_1not availablesample_cond_1
HNNCABCAsample_1not availablesample_cond_1
CABCA(CO)NHNsample_1not availablesample_cond_1
[4,3]D GFT ALI-HCCHsample_1not availablesample_cond_1
[4,3]D GFT ARO-HCCHsample_1not availablesample_cond_1
HABCAB(CO)NHNsample_1not availablesample_cond_1
SIMULTANIOUS HETERONUCLEAR RESOLVED [1H, 1H]-NOESYsample_1not availablesample_cond_1

Software:

NMRPipe v2.5 - processing

DYANA v1.5 - data analysis

AutoStruct v2.0.0 - data analysis

CYANA v2.1 - refinement

CNS v1.1 - refinement

XEASY v1.3.11 - data analysis

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

PDB
GB AAN56344 KEK27680
REF NP_718900 WP_011073216 WP_037420759 WP_037429424
SP Q8EBZ9
AlphaFold Q8EBZ9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks