BMRB Entry 7174

Name: Chemical Shift Assignments for Hdm2 RING finger domain
Published: 2006-10-30

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR7174

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical Shift Assignments for Hdm2 RING finger domain

Deposition date:

2006-06-19

Original release date:

2006-10-30

Authors:

Kostic, Milka; Matt, Theresia; Martinez-Yamout, Maria; Dyson, H.; Wright, Peter

Citation:

Citation: Kostic, Milka; Matt, Theresia; Martinez-Yamout, Maria; Dyson, H.; Wright, Peter. "Solution Structure of Hdm2 C2H2C4 RING, a Domain Critical for Ubiquitination of p53" J. Mol. Biol. 363, 433-450 (2006).
PubMed: 16965791

Assembly members:

Assembly members:
Hdm2 RING finger, polymer, 63 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hdm2 RING finger: SLPLNAIEPCVICQGRPKNG CIVHGKTGHLMACFTCAKKL KKRNKPCPVCRQPIQMIVLT YFP

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	97
15N chemical shifts	65
1H chemical shifts	403

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Hdm2 RING finger domain	1
2	ZINC (II) ION, 1	2
3	ZINC (II) ION, 2	2
4	Hdm2 RING finger domain	1
5	ZINC (II) ION, 1	2
6	ZINC (II) ION, 2	2

Entities:

Entity 1, Hdm2 RING finger domain 63 residues - Formula weight is not available

1

SER

LEU

PRO

LEU

ASN

ALA

ILE

GLU

PRO

CYS

2

VAL

ILE

CYS

GLN

GLY

ARG

PRO

LYS

ASN

GLY

3

CYS

ILE

VAL

HIS

GLY

LYS

THR

GLY

HIS

LEU

4

MET

ALA

CYS

PHE

THR

CYS

ALA

LYS

LEU

5

LYS

ARG

ASN

LYS

PRO

CYS

PRO

VAL

CYS

6

ARG

GLN

PRO

ILE

GLN

MET

ILE

VAL

LEU

THR

7

TYR

PHE

PRO

Entity 2, ZINC (II) ION, 1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: Hdm2 RING finger, [U-13C; U-15N], 0.08 – 0.15 mM; NaCl 150 ± 5 mM; additive arginine 250 ± 5 mM; additive TCEP 0.5 mM; additive ZnSO4 0.005 mM

conditions_1: pH: 7.1; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N_HSQC	sample_1	not available	conditions_1
1H13C_HSQC	sample_1	not available	conditions_1
1H15N_3D_NOESY_HSQC	sample_1	not available	conditions_1
1H15N_3D_TOCSY_HSQC	sample_1	not available	conditions_1
HNCA	sample_1	not available	conditions_1
1H15N_HMQC	sample_1	not available	conditions_1
2D_1H_NOESY	sample_1	not available	conditions_1
2D_1H_TOCSY	sample_1	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Bruker AVANCE 500 MHz
Bruker DRX 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks