BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7170

Title: 1H, 13C, and 15N Chemical Shift Assignments for Bacillus subtilis Hypothetical Protein yvyC: Northeast Structural Genomics Consortium target SR482

Deposition date: 2006-06-15 Original release date: 2015-04-23

Authors: Eletsky, Alexandre; ., Liu; S., .; ., .; 6, .; Haleema, 7; Ma, Li-Chung; ., Xiao; ., .; ., .; 13, .; Gaetano, 14; Szyperski, Thomas

Citation: Eletsky, Alexandre; ., Liu; S., .; ., .; 7170, .; 7, 7170; Li-Chung, 8; Xiao, Rong; ., Liu; ., .; ., B.; 7170, .; 15, 7170; , . "Solution NMR Structure of Bacillus subtilis Protein yvyC"  Proteins: Struct. Funct. Genet. ., .-..

Assembly members:
sr482, polymer, 117 residues, Formula weight is not available

Natural source:   Common Name: B. subtilis' . . Eubacteria 'Not applicable   Taxonomy ID: 1423   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology' . 'E. coli

Entity Sequences (FASTA):
sr482: LNIERLTTLQPVWDRYDTQI HNQKDNDNEVPVHQVSYTNL AEMVGEMNKLLEPSQVHLKF ELHDKLNEYYVKVIEDSTNE VIREIPPKRWLDFYAAMTEF LGLFVDEKKLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts510
1251H chemical shifts

Time Domain Data

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1sr482 monomer1

Entities:

Entity 1, sr482 monomer 117 residues - Formula weight is not available

Contains C-terminal His-tag LEHHHHHH

1   LEU.71705.THR.717011.
2   TRP.717017.GLN.717023.
3   ASP.717029.PRO.717035.
4   TYR.717041.MET.717047.
5   LYS.717053.GLN.717059.
6   GLU.717065.ASN.717071.
7   VAL.717077.ASN.717083.
8   ILE.717089.LEU.717095.
9   MET.7170101.LEU.7170107.
10   LYS.7170113.HIS.7170

Samples:

NC: sr482, [U-100% 13C; U-100% 15N], 1.1 mM; .; .; . % w/w

NC5: sr482, [U-5% 13C; U-100% 15N], 1.1 mM; .; .; . % w/w

conditions_1: pH: 6.5; 298: 0.1 7170

Experiments:

NameSampleSample stateSample conditions
2D 1H15N_HSQCnot availablenot availableconditions_1
nonot availablenot availablenot available
not availablenot availablenot availablenot available
not availablenot available1not available
not availablenot available$conditions_1not available
not availableconditions_1not availablenot available
not availablenot availablenot availablenot available
not availablenot availablenot availablenot available
not availablenot availablenot availablenot available
not availablenot availablenot availablenot available
not availablenot availablenot availablenot available
not availablenot availablenot availablenot available
not availablenot availablenot availablenot available

Software:

VNMR v6.1C, Varian, Inc. - NMR data acqusition

XEASY v1.3.13, xeasy - Spectral analysis

CARA v1.5.3, CARA - Spectral analysis

PROSA v6.0, PROSA - NMR data processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 750 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts