BMRB Entry 7170

Name: 1H, 13C, and 15N Chemical Shift Assignments for Bacillus subtilis Hypothetical Protein yvyC: Northeast Structural Genomics Consortium target SR482
Published: 2015-04-23

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PDB ID: 2hc5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7170
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N Chemical Shift Assignments for Bacillus subtilis Hypothetical Protein yvyC: Northeast Structural Genomics Consortium target SR482

Deposition date:

2006-06-15

Original release date:

2015-04-23

Authors:

Eletsky, Alexandre; Liu, Gaohua; Atreya, Hanudatta; Sukumaran, Dinesh; Wang, Dongyan; Cunningham, Kellie; Janjua, Haleema; Ma, Li-Chung; Xiao, Rong; Liu, Jinfeng; Baran, Michael; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano; Szyperski, Thomas

Citation:

Citation: Eletsky, Alexandre; Liu, Gaohua; Atreya, Hanudatta; Sukumaran, Dinesh; Wang, Dongyan; Cunningham, Kellie; Janjua, Haleema; Ma, Li-Chung; Xiao, Rong; Liu, Jinfeng; Baran, Michael; Acton, Thomas; Rost, Burkhard; Montelione, Gaetano; Szyperski, Thomas. "Solution NMR Structure of Bacillus subtilis Protein yvyC" Proteins: Struct. Funct. Genet. ., .-..

Assembly members:

Assembly members:
sr482, polymer, 117 residues, Formula weight is not available

Natural source:

Natural source: Common Name: B. subtilis Taxonomy ID: 1423 Superkingdom: Eubacteria Kingdom: Not applicable Genus/species: Bacillus subtilis

Experimental source:

Experimental source: Production method: recombinant technology Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
sr482: LNIERLTTLQPVWDRYDTQI HNQKDNDNEVPVHQVSYTNL AEMVGEMNKLLEPSQVHLKF ELHDKLNEYYVKVIEDSTNE VIREIPPKRWLDFYAAMTEF LGLFVDEKKLEHHHHHH

Data sets:

assigned_chemical_shifts
- chem_shift_list_1
spectral_peak_list

Data type	Count
13C chemical shifts	510
15N chemical shifts	125
1H chemical shifts	854

Time Domain Data

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	sr482 monomer	1

Entities:

Entity 1, sr482 monomer 117 residues - Formula weight is not available

Contains C-terminal His-tag LEHHHHHH

1

LEU

ASN

ILE

GLU

ARG

LEU

THR

LEU

GLN

2

PRO

VAL

TRP

ASP

ARG

TYR

ASP

THR

GLN

ILE

3

HIS

ASN

GLN

LYS

ASP

ASN

ASP

ASN

GLU

VAL

4

PRO

VAL

HIS

GLN

VAL

SER

TYR

THR

ASN

LEU

5

ALA

GLU

MET

VAL

GLY

GLU

MET

ASN

LYS

LEU

6

LEU

GLU

PRO

SER

GLN

VAL

HIS

LEU

LYS

PHE

7

GLU

LEU

HIS

ASP

LYS

LEU

ASN

GLU

TYR

8

VAL

LYS

VAL

ILE

GLU

ASP

SER

THR

ASN

GLU

9

VAL

ILE

ARG

GLU

ILE

PRO

LYS

ARG

TRP

10

LEU

ASP

PHE

TYR

ALA

MET

THR

GLU

PHE

11

LEU

GLY

LEU

PHE

VAL

ASP

GLU

LYS

LEU

12

GLU

HIS

Samples:

NC: sr482, [U-100% 13C; U-100% 15N], 1.1 mM; Tris 10 mM; NaCl 100 mM; NaN3 0.02 % w/w

NC5: sr482, [U-5% 13C; U-100% 15N], 1.1 mM; Tris 10 mM; NaCl 100 mM; NaN3 0.02 % w/w

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H15N_HSQC	not available	not available	conditions_1
2D 1H13C_HSQC (ali)	not available	not available	conditions_1
2D 1H13C_HSQC (aro)	not available	not available	conditions_1
2D CT-1H13C_HSQC (ali)	not available	not available	conditions_1
2D CT-1H13C_HSQC (aro)	not available	not available	conditions_1
3D HNCO	not available	not available	conditions_1
(4,3)D GFT CABCA(CO)NHN	not available	not available	conditions_1
(4,3)D GFT HNNCABCA	not available	not available	conditions_1
(4,3)D GFT HABCAB(CO)NHN	not available	not available	conditions_1
(4,3)D GFT HCCH-COSY (ali)	not available	not available	conditions_1
(4,3)D GFT HCCH-COSY (aro)	not available	not available	conditions_1
3D 15N-, 13Cali-, 13Caro-resolved NOESY	not available	not available	conditions_1
2D CT-1H13C_HSQC (methyl)	not available	not available	conditions_1

Software:

VNMR v6.1C, Varian, Inc. - NMR data acqusition

XEASY v1.3.13, xeasy - Spectral analysis

CARA v1.5.3, CARA - Spectral analysis

PROSA v6.0, PROSA - NMR data processing

NMR spectrometers:

Varian INOVA 600 MHz
Varian INOVA 750 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks