BMRB Entry 7161
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7161
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NMR-STAR v3 text file.
XML gzip file.
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Citation: Jayaraman, Bhargavi; Nicholson, Linda. "Backbone Resonance Assignments of Ezrin C ERMAD in a non-covalent complex with Ezrin N FERM" J. Biomol. NMR 36, 63-63 (2006).
PubMed: 17131035
Assembly members:
C ERMAD, polymer, 112 residues, 14580 Da.
N FERM, polymer, 297 residues, 34800 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
C ERMAD: PPVYEPVSYHVQESLQDEGA
EPTGYSAELSSEGIRDDRNE
EKRITEAEKNERVQRQLLTL
SSELSQARDENKRTHNDIIH
NENMRQGRDKYKTLRQIRQG
NTKQRIDEFEAL
N FERM: PKPINVRVTTMDAELEFAIQ
PNTTGKQLFDQVVKTIGLRE
VWYFGLHYVDNKGFPTWLKL
DKKVSAQEVRKENPLQFKFR
AKFYPEDVAEELIQDITQKL
FFLQVKEGILSDEIYCPPET
AVLLGSYAVQAKFGDYNKEV
HKSGYLSSERLIPQRVMDQH
KLTRDQWEDRIQVWHAEHRG
MLKDNAMLEYLKIAQDLEMY
GINYFEIKNKKGTDLWLGVD
ALGLNIYEKDDKLTPKIGFP
WSEIRNISFNDKKFVIKPID
KKAPDFVFYAPRLRINKRIL
QLCMGNHELYMRRRK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 293 |
| 15N chemical shifts | 102 |
| 1H chemical shifts | 102 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | C ERMAD | 1 |
| 2 | N FERM | 2 |
Entities:
Entity 1, C ERMAD 112 residues - 14580 Da.
| 1 | PRO | PRO | VAL | TYR | GLU | PRO | VAL | SER | TYR | HIS | ||||
| 2 | VAL | GLN | GLU | SER | LEU | GLN | ASP | GLU | GLY | ALA | ||||
| 3 | GLU | PRO | THR | GLY | TYR | SER | ALA | GLU | LEU | SER | ||||
| 4 | SER | GLU | GLY | ILE | ARG | ASP | ASP | ARG | ASN | GLU | ||||
| 5 | GLU | LYS | ARG | ILE | THR | GLU | ALA | GLU | LYS | ASN | ||||
| 6 | GLU | ARG | VAL | GLN | ARG | GLN | LEU | LEU | THR | LEU | ||||
| 7 | SER | SER | GLU | LEU | SER | GLN | ALA | ARG | ASP | GLU | ||||
| 8 | ASN | LYS | ARG | THR | HIS | ASN | ASP | ILE | ILE | HIS | ||||
| 9 | ASN | GLU | ASN | MET | ARG | GLN | GLY | ARG | ASP | LYS | ||||
| 10 | TYR | LYS | THR | LEU | ARG | GLN | ILE | ARG | GLN | GLY | ||||
| 11 | ASN | THR | LYS | GLN | ARG | ILE | ASP | GLU | PHE | GLU | ||||
| 12 | ALA | LEU |
Entity 2, N FERM 297 residues - 34800 Da.
| 1 | PRO | LYS | PRO | ILE | ASN | VAL | ARG | VAL | THR | THR | ||||
| 2 | MET | ASP | ALA | GLU | LEU | GLU | PHE | ALA | ILE | GLN | ||||
| 3 | PRO | ASN | THR | THR | GLY | LYS | GLN | LEU | PHE | ASP | ||||
| 4 | GLN | VAL | VAL | LYS | THR | ILE | GLY | LEU | ARG | GLU | ||||
| 5 | VAL | TRP | TYR | PHE | GLY | LEU | HIS | TYR | VAL | ASP | ||||
| 6 | ASN | LYS | GLY | PHE | PRO | THR | TRP | LEU | LYS | LEU | ||||
| 7 | ASP | LYS | LYS | VAL | SER | ALA | GLN | GLU | VAL | ARG | ||||
| 8 | LYS | GLU | ASN | PRO | LEU | GLN | PHE | LYS | PHE | ARG | ||||
| 9 | ALA | LYS | PHE | TYR | PRO | GLU | ASP | VAL | ALA | GLU | ||||
| 10 | GLU | LEU | ILE | GLN | ASP | ILE | THR | GLN | LYS | LEU | ||||
| 11 | PHE | PHE | LEU | GLN | VAL | LYS | GLU | GLY | ILE | LEU | ||||
| 12 | SER | ASP | GLU | ILE | TYR | CYS | PRO | PRO | GLU | THR | ||||
| 13 | ALA | VAL | LEU | LEU | GLY | SER | TYR | ALA | VAL | GLN | ||||
| 14 | ALA | LYS | PHE | GLY | ASP | TYR | ASN | LYS | GLU | VAL | ||||
| 15 | HIS | LYS | SER | GLY | TYR | LEU | SER | SER | GLU | ARG | ||||
| 16 | LEU | ILE | PRO | GLN | ARG | VAL | MET | ASP | GLN | HIS | ||||
| 17 | LYS | LEU | THR | ARG | ASP | GLN | TRP | GLU | ASP | ARG | ||||
| 18 | ILE | GLN | VAL | TRP | HIS | ALA | GLU | HIS | ARG | GLY | ||||
| 19 | MET | LEU | LYS | ASP | ASN | ALA | MET | LEU | GLU | TYR | ||||
| 20 | LEU | LYS | ILE | ALA | GLN | ASP | LEU | GLU | MET | TYR | ||||
| 21 | GLY | ILE | ASN | TYR | PHE | GLU | ILE | LYS | ASN | LYS | ||||
| 22 | LYS | GLY | THR | ASP | LEU | TRP | LEU | GLY | VAL | ASP | ||||
| 23 | ALA | LEU | GLY | LEU | ASN | ILE | TYR | GLU | LYS | ASP | ||||
| 24 | ASP | LYS | LEU | THR | PRO | LYS | ILE | GLY | PHE | PRO | ||||
| 25 | TRP | SER | GLU | ILE | ARG | ASN | ILE | SER | PHE | ASN | ||||
| 26 | ASP | LYS | LYS | PHE | VAL | ILE | LYS | PRO | ILE | ASP | ||||
| 27 | LYS | LYS | ALA | PRO | ASP | PHE | VAL | PHE | TYR | ALA | ||||
| 28 | PRO | ARG | LEU | ARG | ILE | ASN | LYS | ARG | ILE | LEU | ||||
| 29 | GLN | LEU | CYS | MET | GLY | ASN | HIS | GLU | LEU | TYR | ||||
| 30 | MET | ARG | ARG | ARG | LYS |
Samples:
sample_1: C ERMAD, [U-99% 13C; U-99% 15N; U-95% 2H], 0.5 mM; N FERM 0.5 mM; Acetic Acid 10 mM; Sodium Acetate 90 mM; DTT 5 mM; Sodium Azide 15 mM; Protease inhibitors mM
conditions_1: pH: 5.7; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | not available | conditions_1 |
| 3D 15N-edited NOESY (80, 100 and 150 ms mixing times) | sample_1 | not available | conditions_1 |
| HNCO | sample_1 | not available | conditions_1 |
| HNCOCA | sample_1 | not available | conditions_1 |
| HNCACB | sample_1 | not available | conditions_1 |
| HNCA | sample_1 | not available | conditions_1 |
| CBCACONH | sample_1 | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Varian Inova 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAB22341 BAE22456 BAE22499 BAE26602 BAE35312 |
| EMBL | CAA35893 CAA43086 CAB82418 |
| GB | AAA30510 AAA61278 AAH13903 AAH48181 AAH68458 |
| PIR | T47177 |
| REF | NP_001104547 NP_001267458 NP_001273918 NP_003370 NP_033536 |
| SP | P15311 P26040 P31976 P31977 |
| TPG | DAA25983 |
| AlphaFold | P15311 P26040 P31976 P31977 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks