BMRB Entry 7151

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for DLC2-SAM
Deposition date:
2006-06-06
Original release date:
2007-04-18
Authors:
LI, HONGYAN; FUNG, KING-LEUNG; JIN, DONG-YAN; CHUNG, STEPHEN SM; CHING, YICK-PANG; NG, Irene OI-LIN; SZE, KONG-HUNG; KO, BEN CB; SUN, HONGZHE
Citation:

Citation: LI, HONGYAN; FUNG, KING-LEUNG; JIN, DONG-YAN; CHUNG, STEPHEN SM; CHING, YICK-PANG; NG, Irene OI-LIN; SZE, KONG-HUNG; KO, BEN CB; SUN, HONGZHE. "Solution structures, dynamics, and lipid-binding of the sterile alpha-motif domain of the deleted in liver cancer 2"  Proteins 67, 1154-1166 (2007).
PubMed: 17380510

Assembly members:

Assembly members:
SAM domain of Deleted in Liver Cancer 2, polymer, 81 residues, 9321 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pEt-32M

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SAM domain of Deleted in Liver Cancer 2: MHHHHHHSSGLVPRGSQEIE AKEACDWLRAAGFPQYAQLY EDSQFPINIVAVKNDHDFLE KDLVEPLCRRLNTLNKCASM K

Data sets:
Data typeCount
13C chemical shifts318
15N chemical shifts80
1H chemical shifts520

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DLC2-SAM monomer1

Entities:

Entity 1, DLC2-SAM monomer 81 residues - 9321 Da.

1   METHISHISHISHISHISHISSERSERGLY
2   LEUVALPROARGGLYSERGLNGLUILEGLU
3   ALALYSGLUALACYSASPTRPLEUARGALA
4   ALAGLYPHEPROGLNTYRALAGLNLEUTYR
5   GLUASPSERGLNPHEPROILEASNILEVAL
6   ALAVALLYSASNASPHISASPPHELEUGLU
7   LYSASPLEUVALGLUPROLEUCYSARGARG
8   LEUASNTHRLEUASNLYSCYSALASERMET
9   LYS

Samples:

sample_1: SAM domain of Deleted in Liver Cancer 2, [U- 13C; U-15N], 1.3 mM; phosphate buffer 20 mM; DTT" 4 mM; NaN3 0.05%

conditions_1: pH*: 7.13; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D (1H-15N) HSQCsample_1not availableconditions_1
3D (1H-15N) NOESY-HSQCsample_1not availableconditions_1
3D HNCOsample_1not availableconditions_1
3D CBCANHsample_1not availableconditions_1
3D CBCACONHsample_1not availableconditions_1
3D 1H-13C) NOESY-HSQCsample_1not availableconditions_1
3D HCCH-TOCSYsample_1not availableconditions_1
3D HCCH-COSYsample_1not availableconditions_1
3D HBHACONHsample_1not availableconditions_1
2D (1H-13C) HSQCsample_1not availableconditions_1

Software:

NMRPipe -

SPARKY -

CYANA v2.1 -

AMBER v7 -

ProcheckNMR -

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

PDB
GB AAH46563 AAL91649 AIC61070 EAX08531 ELR59815
REF NP_001230395 NP_821075 XP_003314160 XP_003913797 XP_004054406

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks