BMRB Entry 7151
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7151
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: LI, HONGYAN; FUNG, KING-LEUNG; JIN, DONG-YAN; CHUNG, STEPHEN SM; CHING, YICK-PANG; NG, Irene OI-LIN; SZE, KONG-HUNG; KO, BEN CB; SUN, HONGZHE. "Solution structures, dynamics, and lipid-binding of the sterile alpha-motif domain of the deleted in liver cancer 2" Proteins 67, 1154-1166 (2007).
PubMed: 17380510
Assembly members:
SAM domain of Deleted in Liver Cancer 2, polymer, 81 residues, 9321 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Vector: pEt-32M
Entity Sequences (FASTA):
SAM domain of Deleted in Liver Cancer 2: MHHHHHHSSGLVPRGSQEIE
AKEACDWLRAAGFPQYAQLY
EDSQFPINIVAVKNDHDFLE
KDLVEPLCRRLNTLNKCASM
K
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 318 |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 520 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DLC2-SAM monomer | 1 |
Entities:
Entity 1, DLC2-SAM monomer 81 residues - 9321 Da.
| 1 | MET | HIS | HIS | HIS | HIS | HIS | HIS | SER | SER | GLY | ||||
| 2 | LEU | VAL | PRO | ARG | GLY | SER | GLN | GLU | ILE | GLU | ||||
| 3 | ALA | LYS | GLU | ALA | CYS | ASP | TRP | LEU | ARG | ALA | ||||
| 4 | ALA | GLY | PHE | PRO | GLN | TYR | ALA | GLN | LEU | TYR | ||||
| 5 | GLU | ASP | SER | GLN | PHE | PRO | ILE | ASN | ILE | VAL | ||||
| 6 | ALA | VAL | LYS | ASN | ASP | HIS | ASP | PHE | LEU | GLU | ||||
| 7 | LYS | ASP | LEU | VAL | GLU | PRO | LEU | CYS | ARG | ARG | ||||
| 8 | LEU | ASN | THR | LEU | ASN | LYS | CYS | ALA | SER | MET | ||||
| 9 | LYS |
Samples:
sample_1: SAM domain of Deleted in Liver Cancer 2, [U- 13C; U-15N], 1.3 mM; phosphate buffer 20 mM; DTT" 4 mM; NaN3 0.05%
conditions_1: pH*: 7.13; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D (1H-15N) HSQC | sample_1 | not available | conditions_1 |
| 3D (1H-15N) NOESY-HSQC | sample_1 | not available | conditions_1 |
| 3D HNCO | sample_1 | not available | conditions_1 |
| 3D CBCANH | sample_1 | not available | conditions_1 |
| 3D CBCACONH | sample_1 | not available | conditions_1 |
| 3D 1H-13C) NOESY-HSQC | sample_1 | not available | conditions_1 |
| 3D HCCH-TOCSY | sample_1 | not available | conditions_1 |
| 3D HCCH-COSY | sample_1 | not available | conditions_1 |
| 3D HBHACONH | sample_1 | not available | conditions_1 |
| 2D (1H-13C) HSQC | sample_1 | not available | conditions_1 |
Software:
NMRPipe -
SPARKY -
CYANA v2.1 -
AMBER v7 -
ProcheckNMR -
NMR spectrometers:
- Bruker AVANCE 600 MHz
Related Database Links:
| PDB | |
| GB | AAH46563 AAL91649 AIC61070 EAX08531 ELR59815 |
| REF | NP_001230395 NP_821075 XP_003314160 XP_003913797 XP_004054406 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks