BMRB Entry 7149
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7149
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NMR-STAR v3 text file.
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Citation: Greene, Lesley; Wijesinha-Bettoni, Ramani; Redfield, Christina. "Characterization of the molten globule of human serum retinol-binding protein using NMR spectroscopy" Biochemistry 45, 9475-9484 (2006).
PubMed: 16878982
Assembly members:
human serum retinol-binding protein, polymer, 182 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
human serum retinol-binding protein: ERDCRVSSFRVKENFDKARF
SGTWYAMAKKDPEGLFLQDN
IVAEFSVDETGQMSATAKGR
VRLLNNWDVCADMVGTFTDT
EDPAKFKMKYWGVASFLQKG
NDDHWIVDTDYDTYAVQYSC
RLLNLDGTCADSYSFVFSRD
PNGLPPEAQKIVRQRQEELC
LARQYRLIVHNGYCDGRSER
NL
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 174 |
| 1H chemical shifts | 174 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RBP | 1 |
Entities:
Entity 1, RBP 182 residues - Formula weight is not available
| 1 | GLU | ARG | ASP | CYS | ARG | VAL | SER | SER | PHE | ARG | ||||
| 2 | VAL | LYS | GLU | ASN | PHE | ASP | LYS | ALA | ARG | PHE | ||||
| 3 | SER | GLY | THR | TRP | TYR | ALA | MET | ALA | LYS | LYS | ||||
| 4 | ASP | PRO | GLU | GLY | LEU | PHE | LEU | GLN | ASP | ASN | ||||
| 5 | ILE | VAL | ALA | GLU | PHE | SER | VAL | ASP | GLU | THR | ||||
| 6 | GLY | GLN | MET | SER | ALA | THR | ALA | LYS | GLY | ARG | ||||
| 7 | VAL | ARG | LEU | LEU | ASN | ASN | TRP | ASP | VAL | CYS | ||||
| 8 | ALA | ASP | MET | VAL | GLY | THR | PHE | THR | ASP | THR | ||||
| 9 | GLU | ASP | PRO | ALA | LYS | PHE | LYS | MET | LYS | TYR | ||||
| 10 | TRP | GLY | VAL | ALA | SER | PHE | LEU | GLN | LYS | GLY | ||||
| 11 | ASN | ASP | ASP | HIS | TRP | ILE | VAL | ASP | THR | ASP | ||||
| 12 | TYR | ASP | THR | TYR | ALA | VAL | GLN | TYR | SER | CYS | ||||
| 13 | ARG | LEU | LEU | ASN | LEU | ASP | GLY | THR | CYS | ALA | ||||
| 14 | ASP | SER | TYR | SER | PHE | VAL | PHE | SER | ARG | ASP | ||||
| 15 | PRO | ASN | GLY | LEU | PRO | PRO | GLU | ALA | GLN | LYS | ||||
| 16 | ILE | VAL | ARG | GLN | ARG | GLN | GLU | GLU | LEU | CYS | ||||
| 17 | LEU | ALA | ARG | GLN | TYR | ARG | LEU | ILE | VAL | HIS | ||||
| 18 | ASN | GLY | TYR | CYS | ASP | GLY | ARG | SER | GLU | ARG | ||||
| 19 | ASN | LEU |
Samples:
sample_1: human serum retinol-binding protein, [U-15N; U-2H], 0.16 mM; sodium phosphate 5 mM; urea 8 M
conditions_1: pH: 2; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H15N_HSQC | sample_1 | not available | conditions_1 |
Software:
FELIX v2.3, Accelrys - processing
NMR spectrometers:
- Home-built . 750 MHz
Related Database Links:
| PDB | |
| DBJ | BAD16621 BAI46108 |
| EMBL | CAA24959 CAA26553 |
| GB | AAF69622 AAH20633 ABC69164 ABM82307 ABM85484 |
| PRF | 1401251A |
| REF | NP_001038960 NP_006735 XP_002821040 XP_003255281 XP_003904062 |
| SP | P02753 P61641 |
| AlphaFold | P02753 P61641 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks