BMRB Entry 7147

Name: The acute myeloid leukemia fusion protein AML1-ETO targets E-proteins via a PAH-like TAFH domain
Published: 2006-10-30

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PDB ID: 2h7b
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7147
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The acute myeloid leukemia fusion protein AML1-ETO targets E-proteins via a PAH-like TAFH domain

Deposition date:

2006-05-18

Original release date:

2006-10-30

Authors:

Plevin, M.; Zhang, J.; Guo, C.; Roeder, R.; Ikura, M.

Citation:

Citation: Plevin, M.; Zhang, J.; Guo, C.; Roeder, R.; Ikura, M.. "The acute myeloid leukemia fusion protein AML1-ETO targets E proteins via a paired amphipathic helix-like TBP-associated factor homology domain" Proc. Natl. Acad. Sci. U.S.A. 103, 10242-10247 (2006).
PubMed: 16803958

Assembly members:

Assembly members:
Core-binding factor, ML1-ETO, polymer, 105 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Core-binding factor, ML1-ETO: GSARQLSKLKRFLTTLQQFG NDISPEIGERVRTLVLGLVN STLTIEEFHSKLQEATNFPL RPFVIPFLKANLPLLQRELL HAARLAKQNPAQYLAQHEQL LLDAS

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
13C chemical shifts	326
15N chemical shifts	96
1H chemical shifts	768

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Core-binding factor, ML1-ETO	1

Entities:

Entity 1, Core-binding factor, ML1-ETO 105 residues - Formula weight is not available

1

GLY

SER

ALA

ARG

GLN

LEU

SER

LYS

LEU

LYS

2

ARG

PHE

LEU

THR

LEU

GLN

PHE

GLY

3

ASN

ASP

ILE

SER

PRO

GLU

ILE

GLY

GLU

ARG

4

VAL

ARG

THR

LEU

VAL

LEU

GLY

LEU

VAL

ASN

5

SER

THR

LEU

THR

ILE

GLU

PHE

HIS

SER

6

LYS

LEU

GLN

GLU

ALA

THR

ASN

PHE

PRO

LEU

7

ARG

PRO

PHE

VAL

ILE

PRO

PHE

LEU

LYS

ALA

8

ASN

LEU

PRO

LEU

GLN

ARG

GLU

LEU

9

HIS

ALA

ARG

LEU

ALA

LYS

GLN

ASN

PRO

10

ALA

GLN

TYR

LEU

ALA

GLN

HIS

GLU

GLN

LEU

11

LEU

ASP

ALA

SER

Samples:

sample_1: Core-binding factor, ML1-ETO, [U-15N; U-13C], 0.7 mM; sodium phosphate 20 mM; NaCl 50 mM; PMSF 1 mM; AEBSF 1 mM; sodium azide 0.25 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 70 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
NOESY	sample_1	not available	sample_cond_1
4D 13C/15N-separated NOESY	sample_1	not available	sample_cond_1

Software:

CYANA - refinement, structure solution

NMR spectrometers:

Varian UNITYplus 800 MHz

BMRB	16467 7396
PDB	2H7B 2KNH 2PP4
DBJ	BAA03089 BAA03247 BAA03558 BAA03559 BAA03560
EMBL	CAA56311 CAF99400 CAG33073
GB	AAB34819 AAB34820 AAC28931 AAC28932 AAC28933
REF	NP_001070244 NP_001089065 NP_001101923 NP_001102127 NP_001104496
SP	O75081 Q06455 Q61909
AlphaFold	O75081 Q06455 Q61909