BMRB Entry 7133

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7133

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Title:
Backbone 1H and 15N Chemical Shift Assignments for the CheA P4 domain from Thermotoga maritima, in the context of a P3P4 construct
Deposition date:
2006-05-25
Original release date:
2006-10-30
Authors:
Hamel, Damon; Zhou, Hongjun; Starich, Mary; Byrd, Andrew; Dahlquist, Frederick
Citation:

Citation: Hamel, Damon; Zhou, Hongjun; Starich, Mary; Byrd, Andrew; Dahlquist, Frederick. "Chemical-shift-perturbation mapping of the phosphotransfer and catalytic domain interaction in the histidine autokinase CheA from Thermotoga maritima"  Biochemistry 45, 9509-9517 (2006).
PubMed: 16878985

Assembly members:

Assembly members:
P3P4, polymer, 259 residues, 28327 Da.

Natural source:

Natural source:   Common Name: Thermotoga maritima   Taxonomy ID: 243274   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Thermotoga maritima

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
P3P4: KVISQTVRVDIEKLDNLMDL MGELVIARSRILETLKKYNI KELDESLSHLSRITLDLQNV VMKIRMVPISFVFNRFPRMV RDLAKKMNKEVNFIMRGEDT ELDRTFVEEIGEPLLHLLRN AIDHGIEPKEERIAKGKPPI GTLILSARHEGNNVVIEVED DGRGIDKEKIIRKAIEKGLI DESKAATLSDQEILNFLFVP GFSTKEKVSEVSGRGVGMDV VKNVVESLNGSISIESEKDK GTKVTIRLPLTLEHHHHHH

Data sets:
Data typeCount
15N chemical shifts115
1H chemical shifts115

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CheA P3P41

Entities:

Entity 1, CheA P3P4 259 residues - 28327 Da.

Sequence contains CheA residues 290-540. Although not listed, this construct contains a C-terminal histidine tag, LEHHHHHH

1   LYSVALILESERGLNTHRVALARGVALASP
2   ILEGLULYSLEUASPASNLEUMETASPLEU
3   METGLYGLULEUVALILEALAARGSERARG
4   ILELEUGLUTHRLEULYSLYSTYRASNILE
5   LYSGLULEUASPGLUSERLEUSERHISLEU
6   SERARGILETHRLEUASPLEUGLNASNVAL
7   VALMETLYSILEARGMETVALPROILESER
8   PHEVALPHEASNARGPHEPROARGMETVAL
9   ARGASPLEUALALYSLYSMETASNLYSGLU
10   VALASNPHEILEMETARGGLYGLUASPTHR
11   GLULEUASPARGTHRPHEVALGLUGLUILE
12   GLYGLUPROLEULEUHISLEULEUARGASN
13   ALAILEASPHISGLYILEGLUPROLYSGLU
14   GLUARGILEALALYSGLYLYSPROPROILE
15   GLYTHRLEUILELEUSERALAARGHISGLU
16   GLYASNASNVALVALILEGLUVALGLUASP
17   ASPGLYARGGLYILEASPLYSGLULYSILE
18   ILEARGLYSALAILEGLULYSGLYLEUILE
19   ASPGLUSERLYSALAALATHRLEUSERASP
20   GLNGLUILELEUASNPHELEUPHEVALPRO
21   GLYPHESERTHRLYSGLULYSVALSERGLU
22   VALSERGLYARGGLYVALGLYMETASPVAL
23   VALLYSASNVALVALGLUSERLEUASNGLY
24   SERILESERILEGLUSERGLULYSASPLYS
25   GLYTHRLYSVALTHRILEARGLEUPROLEU
26   THRLEUGLUHISHISHISHISHISHIS

Samples:

sample_1: P3P4, [U-2H; U-13C; U-15N], 1.0 ± 0.2 mM; Na2HPO4 50 mM; NaCl 50 mM; sodium azide 0.04%

conditions_1: ionic strength: 100 mM; pH: 7.40; temperature: 323 K

Experiments:

NameSampleSample stateSample conditions
1H15N_TROSY-HSQCsample_1not availableconditions_1
TROISY-HNCAsample_1not availableconditions_1
TROSY-HN(CO)CAsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz
  • Varian Inova 800 MHz

Related Database Links:

PDB
GB AAA96387 AAD35784 ABQ46257 ACB08586 ADA66595
REF NP_228511 WP_004081040 WP_008192691 WP_011942905 WP_012310403
SP Q56310
AlphaFold Q56310

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks