BMRB Entry 7132

Title:
1H, 13C and 15N Chemical Shift Assignments for the CheA P1 domain from Thermotoga maritima
Deposition date:
2006-05-25
Original release date:
2006-10-30
Authors:
Hamel, Damon; Zhou, Hongjun; Starich, Mary; Byrd, Andrew; Dahlquist, Frederick
Citation:

Citation: Hamel, Damon; Zhou, Hongjun; Starich, Mary; Byrd, Andrew; Dahlquist, Frederick. "Chemical-shift-perturbation mapping of the phosphotransfer and catalytic domain interaction in the histidine autokinase CheA from Thermotoga maritima."  Biochemistry 45, 9509-9517 (2006).
PubMed: 16878985

Assembly members:

Assembly members:
P1, polymer, 131 residues, 14690 Da.

Natural source:

Natural source:   Common Name: Thermotoga maritima   Taxonomy ID: 243274   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Thermotoga maritima

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts276
15N chemical shifts122
1H chemical shifts358

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CheA P11

Entities:

Entity 1, CheA P1 131 residues - 14690 Da.

1   METMETGLUGLUTYRLEUGLYVALPHEVAL
2   ASPGLUTHRLYSGLUTYRLEUGLNASNLEU
3   ASNASPTHRLEULEUGLULEUGLULYSASN
4   PROGLUASPMETGLULEUILEASNGLUALA
5   PHEARGALALEUHISTHRLEULYSGLYMET
6   ALAGLYTHRMETGLYPHESERSERMETALA
7   LYSLEUCYSHISTHRLEUGLUASNILELEU
8   ASPLYSALAARGASNSERGLUILELYSILE
9   THRSERASPLEULEUASPLYSILEPHEALA
10   GLYVALASPMETILETHRARGMETVALASP
11   LYSILEVALSERGLUGLYSERASPASPILE
12   GLYGLUASNILEASPVALPHESERASPTHR
13   ILELYSSERPHEALASERSERGLYLYSGLU
14   LYS

Samples:

sample_1: P1, [U-98% 13C; U-98% 15N], 1.5 ± 0.2 mM; Na2HPO4 50 mM; NaCl 50 mM; sodium azide 0.04%

conditions_1: ionic strength: 100 mM; pH: 7.40; temperature: 323 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
CBCA(CO)NNHsample_1not availableconditions_1
ccc-tocsysample_1not availableconditions_1
hcch-tocsysample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

BMRB 17651
PDB
GB AAA96387 AAD35784 ABQ46257 ACB08586 ADA66595
REF NP_228511 WP_004081040 WP_008192691 WP_011942905 WP_012310403
SP Q56310
AlphaFold Q56310

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks