BMRB Entry 7130

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7130

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Title:
Backbone NMR assignment of the 29.6 kDa Rhodanese protein from Azotobacter vinelandii
Deposition date:
2006-05-24
Original release date:
2006-10-02
Authors:
Gallo, Mariana; Melis, Riccardo; Paci, Maurizio; Melino, Sonia; Cicero, Daniel
Citation:

Citation: Gallo, Mariana; Melino, Sonia; Melis, Riccardo; Paci, Maurizio; Cicero, Daniel. "Backbone NMR assignment of the 29.6 kDa Rhodanese protein from Azotobacter vinelandii"  J. Biomol. NMR 36, 73-73 (2006).
PubMed: 16933019

Assembly members:

Assembly members:
Rhodanese, polymer, 271 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Azotobacter vinelandii   Taxonomy ID: 354   Superkingdom: Eubacteria   Kingdom: Metazoa   Genus/species: Azotobacter vinelandii

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Rhodanese: MDDFASLPLVIEPADLQARL SAPELILVDLTSAARYAEGH IPGARFVDPKRTQLGQPPAP GLQPPREQLESLFGELGHRP EAVYVVYDDEGGGWAGRFIW LLDVIGQQRYHYLNGGLTAW LAEDRPLSRELPAPAGGPVA LSLHDEPTASRDYLLGRLGA ADLAIWDARSPQEYRGEKVL AAKGGHIPGAVNFEWTAAMD PSRALRIRTDIAGRLEELGI TPDKEIVTHCQTHHRSGLTY LIAKALGYPRVKGYAGSWGE WGNHPDTPVEL

Data sets:
Data typeCount
13C chemical shifts720
15N chemical shifts233
1H chemical shifts234

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Rhodanese1

Entities:

Entity 1, Rhodanese 271 residues - Formula weight is not available

1   METASPASPPHEALASERLEUPROLEUVAL
2   ILEGLUPROALAASPLEUGLNALAARGLEU
3   SERALAPROGLULEUILELEUVALASPLEU
4   THRSERALAALAARGTYRALAGLUGLYHIS
5   ILEPROGLYALAARGPHEVALASPPROLYS
6   ARGTHRGLNLEUGLYGLNPROPROALAPRO
7   GLYLEUGLNPROPROARGGLUGLNLEUGLU
8   SERLEUPHEGLYGLULEUGLYHISARGPRO
9   GLUALAVALTYRVALVALTYRASPASPGLU
10   GLYGLYGLYTRPALAGLYARGPHEILETRP
11   LEULEUASPVALILEGLYGLNGLNARGTYR
12   HISTYRLEUASNGLYGLYLEUTHRALATRP
13   LEUALAGLUASPARGPROLEUSERARGGLU
14   LEUPROALAPROALAGLYGLYPROVALALA
15   LEUSERLEUHISASPGLUPROTHRALASER
16   ARGASPTYRLEULEUGLYARGLEUGLYALA
17   ALAASPLEUALAILETRPASPALAARGSER
18   PROGLNGLUTYRARGGLYGLULYSVALLEU
19   ALAALALYSGLYGLYHISILEPROGLYALA
20   VALASNPHEGLUTRPTHRALAALAMETASP
21   PROSERARGALALEUARGILEARGTHRASP
22   ILEALAGLYARGLEUGLUGLULEUGLYILE
23   THRPROASPLYSGLUILEVALTHRHISCYS
24   GLNTHRHISHISARGSERGLYLEUTHRTYR
25   LEUILEALALYSALALEUGLYTYRPROARG
26   VALLYSGLYTYRALAGLYSERTRPGLYGLU
27   TRPGLYASNHISPROASPTHRPROVALGLU
28   LEU

Samples:

sample_1: Rhodanese, [U-95% 13C; U-90% 15N; U-70% 2H], 0.5 ± 0.1 mM; deuterated Tris 100 ± 1.0 mM; NaCl 300 ± 5.0 mM; perdeuterated b-octil glucoside 0.2 ± 0.05 %

conditions_1: ionic strength: 0.3 M; pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
trosy-HNCOsample_1not availableconditions_1
trosy-HNCAsample_1not availableconditions_1
trosy-HNcoCAsample_1not availableconditions_1
trosy-HNCOCACBsample_1not availableconditions_1
trosy-HNCACBsample_1not availableconditions_1
trosy-HNcaCOsample_1not availableconditions_1
15Nedited 1H1H NOESYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

    Related Database Links:

    PDB
    GB AAB03239 ACO76991 AGK13937 AGK18661
    REF WP_012699416 YP_002797966 YP_007891778 YP_007896826
    SP P52197
    AlphaFold P52197

    Download HSQC peak lists in one of the following formats:
    CSV: Backbone or all simulated peaks
    SPARKY: Backbone or all simulated peaks