BMRB Entry 7118

Name: Solution structure of neurabin SAM domain
Published: 2007-09-24

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7118

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of neurabin SAM domain

Deposition date:

2006-05-17

Original release date:

2007-09-24

Authors:

Ju, Tingting; Peti, Wolfgang

Citation:

Citation: Ju, Tingting; Ragusa, M.J.; Hudak, J.; Nairn, A.C.; Peti, Wolfgang. "Structural characterization of the neurabin sterile alpha motif domain" Proteins 69, 192-198 (2007).
PubMed: 17600833

Assembly members:

Assembly members:
Neurabin SAM domain, polymer, 74 residues, 8341.6 Da.

Natural source:

Natural source: Common Name: Norway Rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Animalia Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Neurabin SAM domain: GHMVHEWSVQQVSHWLVGLS LDQYVSEFSAQNISGEQLLQ LDGNKLKALGMTSSQDRALV KKKLKEMKMSLEKA

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	303
15N chemical shifts	83
1H chemical shifts	530

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Neurabin SAM domain monomer	1

Entities:

Entity 1, Neurabin SAM domain monomer 74 residues - 8341.6 Da.

1

GLY

HIS

MET

VAL

HIS

GLU

TRP

SER

VAL

GLN

2

GLN

VAL

SER

HIS

TRP

LEU

VAL

GLY

LEU

SER

3

LEU

ASP

GLN

TYR

VAL

SER

GLU

PHE

SER

ALA

4

GLN

ASN

ILE

SER

GLY

GLU

GLN

LEU

GLN

5

LEU

ASP

GLY

ASN

LYS

LEU

LYS

ALA

LEU

GLY

6

MET

THR

SER

GLN

ASP

ARG

ALA

LEU

VAL

7

LYS

LEU

LYS

GLU

MET

LYS

MET

SER

8

LEU

GLU

LYS

ALA

Samples:

sample_1: Neurabin SAM domain, [U-95% 13C; U-99% 15N], 1.5 ± 0.1 mM; NaPi 20 mM; NaCl 50 mM; D2O 100%

sample_2: Neurabin SAM domain, [U-99% 15N], 1.5 ± 0.1 mM; NaPi 20 mM; NaCl 50 mM; D2O 100%

sample_3: Neurabin SAM domain 1.5 ± 0.1 mM; NaPi 20 mM; NaCl 50 mM; D2O 100%

conditions_1: pH: 6.8; temperature: 298 K

conditions_2: pH: 6.8; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
15N_edited_NOESY	sample_2	not available	conditions_1
13C_edited_NOESY	sample_1	not available	conditions_1
2D_NOESY	sample_3	not available	conditions_2

Software:

TOPSPIN v1.3, Bruker - data acquisition and processing

CARA v1.5, . - Data analysis

ATNOS v1.1, . - automated peak assignments

CYANA v2.0, LAS - structure calculation

CNS v1.1, . - structure refinement

NMR spectrometers:

Bruker AVANCE II 500 MHz

PDB	2GLE
GB	AAC53454 EDM15014 EDM15015 EDM15016
REF	NP_445925 XP_006236119 XP_006236120 XP_006236123 XP_006236125
SP	O35867
AlphaFold	O35867