BMRB Entry 7115

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7115

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Title:
1H, 13C and 15N resonance assignments of the apo-form of human eIF4E
Deposition date:
2006-05-17
Original release date:
2006-09-11
Authors:
Volpon, Laurent; Osborne, Michael; Borden, Katherine
Citation:

Citation: Volpon, Laurent; Osborne, Michael; Borden, Katherine. "NMR assignment of human eukaryotic translation initiation factor 4E (eIF4E) in its cap-free form"  J. Biomol. NMR 36, 65-65 (2006).
PubMed: 16871426

Assembly members:

Assembly members:
eIF4E, polymer, 217 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
eIF4E: MATVEPETTPTPNPPTTEEE KTESNQEVANPEHYIKHPLQ NRWALWFFKNDKSKTWQANL RLISKFDTVEDFWALYNHIQ LSSNLMPGCDYSLFKDGIEP MWEDEKNKRGGRWLITLNKQ QRRSDLDRFWLETLLCLIGE SFDDYSDDVCGAVVNVRAKG DKIAIWTTECENREAVTHIG RVYKERLGLPPKIVIGYQSH ADTATKSGSTTKNRFVV

Data sets:
Data typeCount
13C chemical shifts553
15N chemical shifts213
1H chemical shifts927

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1eIF4E1

Entities:

Entity 1, eIF4E 217 residues - Formula weight is not available

1   METALATHRVALGLUPROGLUTHRTHRPRO
2   THRPROASNPROPROTHRTHRGLUGLUGLU
3   LYSTHRGLUSERASNGLNGLUVALALAASN
4   PROGLUHISTYRILELYSHISPROLEUGLN
5   ASNARGTRPALALEUTRPPHEPHELYSASN
6   ASPLYSSERLYSTHRTRPGLNALAASNLEU
7   ARGLEUILESERLYSPHEASPTHRVALGLU
8   ASPPHETRPALALEUTYRASNHISILEGLN
9   LEUSERSERASNLEUMETPROGLYCYSASP
10   TYRSERLEUPHELYSASPGLYILEGLUPRO
11   METTRPGLUASPGLULYSASNLYSARGGLY
12   GLYARGTRPLEUILETHRLEUASNLYSGLN
13   GLNARGARGSERASPLEUASPARGPHETRP
14   LEUGLUTHRLEULEUCYSLEUILEGLYGLU
15   SERPHEASPASPTYRSERASPASPVALCYS
16   GLYALAVALVALASNVALARGALALYSGLY
17   ASPLYSILEALAILETRPTHRTHRGLUCYS
18   GLUASNARGGLUALAVALTHRHISILEGLY
19   ARGVALTYRLYSGLUARGLEUGLYLEUPRO
20   PROLYSILEVALILEGLYTYRGLNSERHIS
21   ALAASPTHRALATHRLYSSERGLYSERTHR
22   THRLYSASNARGPHEVALVAL

Samples:

sample_1: eIF4E, [U-95% 13C; U-95% 15N], 0.3 mM; NaCl 100 mM

sample_2: eIF4E, [U-95% 2H; U-95% 13C; U-95% 15N], 0.3 mM; NaCl 100 mM

conditions_1: ionic strength: 0.1 M; pH: 7.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availableconditions_1
HNCOnot availablenot availableconditions_1
3D HCCH-COSYnot availablenot availableconditions_1
3D 15N-edited NOESYnot availablenot availableconditions_1
3D 13C-edited NOESYnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAC38660 BAE00892 BAE39250 BAE87828 BAE88752
EMBL CAA43943 CAA58316 CAL92193
GB AAA37545 AAC13647 AAF66991 AAH10759 AAH12611
REF NP_001095180 NP_001124150 NP_001233399 NP_001253148 NP_001271630
SP P06730 P29338 P63073 P63074 Q9N0T5
TPG DAA28826
AlphaFold P06730 P29338 P63073 P63074 Q9N0T5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks