BMRB Entry 7113

Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR7113

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Title:
Chemical Shift Assignments for the First Immunoglobulin Domain of Myotilin
Deposition date:
2006-05-17
Original release date:
2009-05-27
Authors:
Heikkinen, Outi; Kilpelainen, Ilkka; Permi, Perttu; Ylanne, Jari; Koskela, Harri; Heikkinen, Sami
Citation:

Citation: Heikkinen, Outi; Permi, Perttu; Koskela, Harri; Carpen, Olli; Ylanne, Jari; Kilpelainen, Ilkka. "Solution Structure of The First Immunoglobulin Domain of Myotilin"  J. Biomol. NMR 44, 107-112 (2009).
PubMed: 19418025

Assembly members:

Assembly members:
The First Immunoglobulin Domain of Myotilin, polymer, 100 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Homo Sapiens   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: C1-5A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
The First Immunoglobulin Domain of Myotilin: GAMGPPRFIQVPENMSIDEG RFCRMDFKVSGLPAPDVSWY LNGRTVQSDDLHKMIVSEKG LHSLIFEVVRASDAGAYACV AKNRAGEATFTVQLDVLAKE

Data sets:
Data typeCount
13C chemical shifts397
15N chemical shifts92
1H chemical shifts692

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Myotilin_Ig11

Entities:

Entity 1, Myotilin_Ig1 100 residues - Formula weight is not available

1   GLYALAMETGLYPROPROARGPHEILEGLN
2   VALPROGLUASNMETSERILEASPGLUGLY
3   ARGPHECYSARGMETASPPHELYSVALSER
4   GLYLEUPROALAPROASPVALSERTRPTYR
5   LEUASNGLYARGTHRVALGLNSERASPASP
6   LEUHISLYSMETILEVALSERGLULYSGLY
7   LEUHISSERLEUILEPHEGLUVALVALARG
8   ALASERASPALAGLYALATYRALACYSVAL
9   ALALYSASNARGALAGLYGLUALATHRPHE
10   THRVALGLNLEUASPVALLEUALALYSGLU

Samples:

sample_1: Myotilin_Ig1, [U-U-15N; U-13C], 0.5 mM; Phosphate Buffer 20 mM; Sodium Chloride 140 mM; Dithiothreitol 1 mM

conditions_1: pH: 6.7; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCsample_1not availableconditions_1
1H13C_HSQCsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
HNCOCAsample_1not availableconditions_1
HNCOCACBsample_1not availableconditions_1
iHNCAsample_1not availableconditions_1
CCCONHsample_1not availableconditions_1
HCCCOHNsample_1not availableconditions_1
HCCH-COSYsample_1not availableconditions_1
1H13C_NOESY_HSQCsample_1not availableconditions_1
1H15N_NOESY_HSQCsample_1not availableconditions_1
HNCOsample_1not availableconditions_1
HNCACOsample_1not availableconditions_1

Software:

VNMR v6.1, Varian Inc. - Data aquisition and processing

AutoAssign - automated peak assignments

SPARKY v3.106 - Spectra visualization and chemical shift assignment

NMR spectrometers:

  • Varian Unity 800 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks