BMRB Entry 7106

Name: NMR Assignment of human RGS18 (regulator of G-protein signalling)
Published: 2006-08-15

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PDB ID: 2jm5
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7106
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Assignment of human RGS18 (regulator of G-protein signalling)

Deposition date:

2006-05-11

Original release date:

2006-08-15

Authors:

Higman, Victoria; Leidert, Martina; Elkins, Jon; Soundararajan, Meera; Brockmann, Christoph; Schmieder, Peter; Sundstrom, Michael; Arrowsmith, Cheryl; Weigelt, Johan; Edwards, Aled; Diehl, Annette; Oschkinat, Hartmut; Ball, Linda

Citation:

Citation: Higman, Victoria; Leidert, Martina; Diehl, Annette; Elkins, Jonathan; Soundararajan, Meera; Oschkinat, Hartmut; Ball, Linda. "NMR assignment of Human RGS18" J. Biomol. NMR 36, 72-72 (2006).
PubMed: 16964532

Assembly members:

Assembly members:
RGS18, polymer, 151 residues, 17760 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RGS18: SMVSPEEAVKWGESFDKLLS HRDGLEAFTRFLKTEFSEEN IEFWIACEDFKKSKGPQQIH LKAKAIYEKFIQTDAPKEVN LDFHTKEVITNSITQPTLHS FDAAQSRVYQLMEQDSYTRF LKSDIYLDLMEGRPQRPTNL RRRSRSFTCNE

Data sets:

assigned_chemical_shifts
- chem_shift_list_1

Data type	Count
13C chemical shifts	601
15N chemical shifts	134
1H chemical shifts	924

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RGS18	1

Entities:

Entity 1, RGS18 151 residues - 17760 Da.

1

SER

MET

VAL

SER

PRO

GLU

ALA

VAL

LYS

2

TRP

GLY

GLU

SER

PHE

ASP

LYS

LEU

SER

3

HIS

ARG

ASP

GLY

LEU

GLU

ALA

PHE

THR

ARG

4

PHE

LEU

LYS

THR

GLU

PHE

SER

GLU

ASN

5

ILE

GLU

PHE

TRP

ILE

ALA

CYS

GLU

ASP

PHE

6

LYS

SER

LYS

GLY

PRO

GLN

ILE

HIS

7

LEU

LYS

ALA

LYS

ALA

ILE

TYR

GLU

LYS

PHE

8

ILE

GLN

THR

ASP

ALA

PRO

LYS

GLU

VAL

ASN

9

LEU

ASP

PHE

HIS

THR

LYS

GLU

VAL

ILE

THR

10

ASN

SER

ILE

THR

GLN

PRO

THR

LEU

HIS

SER

11

PHE

ASP

ALA

GLN

SER

ARG

VAL

TYR

GLN

12

LEU

MET

GLU

GLN

ASP

SER

TYR

THR

ARG

PHE

13

LEU

LYS

SER

ASP

ILE

TYR

LEU

ASP

LEU

MET

14

GLU

GLY

ARG

PRO

GLN

ARG

PRO

THR

ASN

LEU

15

ARG

SER

ARG

SER

PHE

THR

CYS

ASN

16

GLU

Samples:

sample_1: RGS18, [U-95% 15N], 1.0 mM; Sodium Phosphate buffer 20 mM; sodium chloride 50 mM

sample_2: RGS18, [U-95% 15N; U-95% 13C], 1.0 mM; Sodium Phosphate buffer 20 mM; sodium chloride 50 mM

sample_3: RGS18, [U-95% 15N; U-95% 13C], 1.0 mM; Sodium Phosphate buffer 20 mM; sodium chloride 50 mM; D2O 100%

conditions_1: pH: 6.0; temperature: 297.0 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H15N HSQC	sample_1	not available	conditions_1
3D HNCO	sample_2	not available	conditions_1
3D CBCA(CO)NNH	sample_2	not available	conditions_1
3D CBCACONNH	sample_2	not available	conditions_1
3D HN(CA)CO	sample_2	not available	conditions_1
3D CCC(CO)NNH	sample_2	not available	conditions_1
3D H(CCO)NNH	sample_2	not available	conditions_1
3D HBHA(CO)NNH	sample_2	not available	conditions_1
3D HCCH TOCSY	sample_2	not available	conditions_1
3D HCCH COSY	sample_2	not available	conditions_1
3D 15N-NOESY-HSQC	sample_2	not available	conditions_1
3D 13C-HMQC-NOESY	sample_2	not available	conditions_1
3D 13C-NOESY-HSQC	sample_2	not available	conditions_1
1H13C-HMQC	sample_3	not available	conditions_1
2D TOCSY	not available	not available	conditions_1

Software:

XWIN-NMR v2.6, Bruker - processing

ANALYSIS v1.0.9-10 - assignment

CYANA v2.0 - automated NOESY peak assignment

X-PLOR NIH - structure refinement

NMR spectrometers:

Bruker DRX 600 MHz
Bruker DMX 750 MHz
Bruker DRX 600 MHz

PDB	2DLV 2JM5 2OWI
DBJ	BAF84704 BAG37770 BAG51866 BAI46194
GB	AAF80227 AAH20632 AAK58589 ABM82319 ABM85495
REF	NP_570138 XP_001113061 XP_002809731 XP_003264531 XP_003820638
SP	Q9NS28
AlphaFold	Q9NS28 Q9NS28