BMRB Entry 7105

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7105

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Title:
1H, 13C and 15N chemical shift assignments for SRY.B in complex with 16-mer DNA
Deposition date:
2006-05-09
Original release date:
2006-08-07
Authors:
Stott, Katherine; Tang, George; Lee, Keng-Boon; Thomas, Jean
Citation:

Citation: Stott, Katherine; Tang, George; Lee, Keng-Boon; Thomas, Jean. "Structure of a Complex of Tandem HMG Boxes and DNA"  J. Mol. Biol. 360, 90-104 (2006).
PubMed: 16813837

Assembly members:

Assembly members:
SRY.B, polymer, 159 residues, Formula weight is not available
16-mer DNA trand 1, polymer, 16 residues, Formula weight is not available
16-mer DNA strand 2, polymer, 16 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SRY.B: VQDRVKRPMNAFIVWSRDQR RKMALENPRMRNSEISKQLG YQWKMLTEAEKWPFFQEAQK LQAMHREKYPNYKYRKGETK KKFKDPNAPKRPPSAFFLFC SEYRPKIKGEHPGLSIGDVA KKLGEMWNNTAADDKQPYEK KAAKLKEKYEKDIAAYRAK
16-mer DNA trand 1: GGGATCTAAACAATGC
16-mer DNA strand 2: GCATTGTTTAGATCCC

Data sets:
Data typeCount
13C chemical shifts619
15N chemical shifts163
1H chemical shifts1493

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SRY.B in complex with 16-mer DNA1
2SRY.B in complex with 16-mer DNA strand 12
3SRY.B in complex with 16-mer DNA strand 23

Entities:

Entity 1, SRY.B in complex with 16-mer DNA 159 residues - Formula weight is not available

1   VALGLNASPARGVALLYSARGPROMETASN
2   ALAPHEILEVALTRPSERARGASPGLNARG
3   ARGLYSMETALALEUGLUASNPROARGMET
4   ARGASNSERGLUILESERLYSGLNLEUGLY
5   TYRGLNTRPLYSMETLEUTHRGLUALAGLU
6   LYSTRPPROPHEPHEGLNGLUALAGLNLYS
7   LEUGLNALAMETHISARGGLULYSTYRPRO
8   ASNTYRLYSTYRARGLYSGLYGLUTHRLYS
9   LYSLYSPHELYSASPPROASNALAPROLYS
10   ARGPROPROSERALAPHEPHELEUPHECYS
11   SERGLUTYRARGPROLYSILELYSGLYGLU
12   HISPROGLYLEUSERILEGLYASPVALALA
13   LYSLYSLEUGLYGLUMETTRPASNASNTHR
14   ALAALAASPASPLYSGLNPROTYRGLULYS
15   LYSALAALALYSLEULYSGLULYSTYRGLU
16   LYSASPILEALAALATYRARGALALYS

Entity 2, SRY.B in complex with 16-mer DNA strand 1 16 residues - Formula weight is not available

1   DGDGDGDADTDCDTDADADA
2   DCDADADTDGDC

Entity 3, SRY.B in complex with 16-mer DNA strand 2 16 residues - Formula weight is not available

1   DGDCDADTDTDGDTDTDTDA
2   DGDADTDCDCDC

Samples:

sample_1: SRY.B, [U-99% 13C; U-99% 15N], 1 mM; Sodium phosphate 10 mM; EDTA 1 mM; DTT, [U-98% 2H], 2 mM

conditions_1: pH: 6.8; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D HSQCsample_1not availableconditions_1
3D HN(CO)CAsample_1not availableconditions_1
3D HNCAsample_1not availableconditions_1
3D CBCA(CO)NHsample_1not availableconditions_1
3D HNCACBsample_1not availableconditions_1
3D HNCOsample_1not availableconditions_1
3D(H)CC(CO)NH-TOCSYsample_1not availableconditions_1
3D TOCSY-15N-HSQCsample_1not availableconditions_1
3D NOESY-15N-HSQCsample_1not availableconditions_1
3D HC(C)H-TOCSYsample_1not availableconditions_1
3D NOESY-13C-HSQCsample_1not availableconditions_1
3D HNHAsample_1not availableconditions_1
2D TOCSYsample_1not availableconditions_1
2D DQF-COSYsample_1not availableconditions_1
2D NOESYsample_1not availableconditions_1
2D [F1]-13C/15N-filtered TOCSYsample_1not availableconditions_1
2D [F1]-13C/15N-filtered DQF COSYsample_1not availableconditions_1
2D [F1]-13C/15N-filtered NOESYsample_1not availableconditions_1
3D [F1]-13C/15N-filtered NOESY-13C-HSQCsample_1not availableconditions_1
2D [F1,F2]-13C/15N-filtered NOESYsample_1not availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 800 MHz

Related Database Links:

PDB
DBJ BAG51867
GB EGW08959
REF XP_004384214 XP_004435356 XP_004680239 XP_005902227 XP_007956028

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks