BMRB Entry 7094

Name: 1H and 15N Chemical Shift Assignments for the Acylphosphatase from Eschaerichia Coli
Published: 2006-10-30

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7094

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H and 15N Chemical Shift Assignments for the Acylphosphatase from Eschaerichia Coli

Deposition date:

2006-05-03

Original release date:

2006-10-30

Authors:

Pagano, Katiuscia; Corazza, Alessandra; Viglino, Paolo; Esposito, Gennaro

Citation:

Citation: Pagano, K.; Ramazzotti, M.; Viglino, P.; Esposito, G.; Degl'innocenti, D.; Taddei, N.; Corazza, A.. "NMR solution structure of the acylphosphatase from Escherichia coli." J. Biomol. NMR 36, 199-204 (2006).
PubMed: 17021943

Assembly members:

Assembly members:
Eschaerichia Coli Acylphosphatase, polymer, 92 residues, 10581 Da.

Natural source:

Natural source: Common Name: Eschaerichia Coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Eschaerichia coli

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Eschaerichia Coli Acylphosphatase: MSKVCIIAWVYGRVQGVGFR YTTQYEAKRLGLTGYAKNLD DGSVEVVACGEEGQVEKLMQ WLKSGGPRSARVERVLSEPH HPSGELTDFRIR

Data sets:

assigned_chemical_shifts
- chem_shift_list_1
coupling_constants
- J_coupling_list_1

Data type	Count
15N chemical shifts	100
1H chemical shifts	486
coupling constants	59

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	EColi	1

Entities:

Entity 1, EColi 92 residues - 10581 Da.

1

MET

SER

LYS

VAL

CYS

ILE

ALA

TRP

VAL

2

TYR

GLY

ARG

VAL

GLN

GLY

VAL

GLY

PHE

ARG

3

TYR

THR

GLN

TYR

GLU

ALA

LYS

ARG

LEU

4

GLY

LEU

THR

GLY

TYR

ALA

LYS

ASN

LEU

ASP

5

ASP

GLY

SER

VAL

GLU

VAL

ALA

CYS

GLY

6

GLU

GLY

GLN

VAL

GLU

LYS

LEU

MET

GLN

7

TRP

LEU

LYS

SER

GLY

PRO

ARG

SER

ALA

8

ARG

VAL

GLU

ARG

VAL

LEU

SER

GLU

PRO

HIS

9

HIS

PRO

SER

GLY

GLU

LEU

THR

ASP

PHE

ARG

10

ILE

ARG

Samples:

sample_1: Eschaerichia Coli Acylphosphatase 0.5 mM

sample_2: Eschaerichia Coli Acylphosphatase, [U-95% 15N], 0.5 mM

conditions_1: pH: 4.95; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H TOCSY	not available	not available	conditions_1
2D 1H NOESY	not available	not available	conditions_1
2D 1H DQF-COSY	not available	not available	conditions_1
2D 1H 15N HSQC	not available	not available	conditions_1
3D 1H 15N NOESY-HSQC	not available	not available	conditions_1

Software:

No software information available

NMR spectrometers:

Bruker Avance 500.1323 MHz

PDB	2GV1
DBJ	BAA35733 BAB34475 BAI24411 BAI29861 BAI34990
EMBL	CAQ31496 CAQ88634 CAQ97873 CAR12367 CAR18304
GB	AAC74054 AAG55454 AAN42598 AAN79574 AAP16484
REF	NP_286843 NP_309079 NP_415488 NP_706891 NP_753031
SP	P0AB65 P0AB66 Q31YN1 Q83LL9 Q8FJ70
AlphaFold	P0AB65 P0AB66 Q31YN1 Q83LL9 Q8FJ70