BMRB Entry 7086
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7086
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Wu, Bin; Yee, Adelina; Lukin, Jonathan; Lemak, Alexander; Ramelot, Theresa; Semesi, Anthony; Kennedy, Michael; Arrowsmith, Cheryl. "Assignment of hypothetical protein tm1012 from Thermotoga maritima/ Northeast
Structural Genomics Consortium Target VT128/ Ontario Center for Structural
Proteomics Target tm1012" The BMRB entry is the only known published source for the data..
Assembly members:
hypothetical protein tm1012, polymer, 158 residues, 18630 Da.
Natural source: Common Name: Thermotoga maritima Taxonomy ID: 2303 Superkingdom: Bacteria Kingdom: not available Genus/species: thermotoga maritima
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET15B
Entity Sequences (FASTA):
hypothetical protein tm1012: MIRPEYLRVLRKIYDRLKNE
KVNWVVTGSLSFALQGVPVE
VHDIDIQTDEEGAYEIERIF
SEFVSKKVRFSSTEKICSHF
GELIIDGIKVEIMGDIRKRL
EDGTWEDPVDLNKYKRFVET
HGMKIPVLSLEYEYQAYLKL
GRVEKAETLRKWLNERKG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 663 |
| 15N chemical shifts | 147 |
| 1H chemical shifts | 1103 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | tm1012 | 1 |
Entities:
Entity 1, tm1012 158 residues - 18630 Da.
| 1 | MET | ILE | ARG | PRO | GLU | TYR | LEU | ARG | VAL | LEU | ||||
| 2 | ARG | LYS | ILE | TYR | ASP | ARG | LEU | LYS | ASN | GLU | ||||
| 3 | LYS | VAL | ASN | TRP | VAL | VAL | THR | GLY | SER | LEU | ||||
| 4 | SER | PHE | ALA | LEU | GLN | GLY | VAL | PRO | VAL | GLU | ||||
| 5 | VAL | HIS | ASP | ILE | ASP | ILE | GLN | THR | ASP | GLU | ||||
| 6 | GLU | GLY | ALA | TYR | GLU | ILE | GLU | ARG | ILE | PHE | ||||
| 7 | SER | GLU | PHE | VAL | SER | LYS | LYS | VAL | ARG | PHE | ||||
| 8 | SER | SER | THR | GLU | LYS | ILE | CYS | SER | HIS | PHE | ||||
| 9 | GLY | GLU | LEU | ILE | ILE | ASP | GLY | ILE | LYS | VAL | ||||
| 10 | GLU | ILE | MET | GLY | ASP | ILE | ARG | LYS | ARG | LEU | ||||
| 11 | GLU | ASP | GLY | THR | TRP | GLU | ASP | PRO | VAL | ASP | ||||
| 12 | LEU | ASN | LYS | TYR | LYS | ARG | PHE | VAL | GLU | THR | ||||
| 13 | HIS | GLY | MET | LYS | ILE | PRO | VAL | LEU | SER | LEU | ||||
| 14 | GLU | TYR | GLU | TYR | GLN | ALA | TYR | LEU | LYS | LEU | ||||
| 15 | GLY | ARG | VAL | GLU | LYS | ALA | GLU | THR | LEU | ARG | ||||
| 16 | LYS | TRP | LEU | ASN | GLU | ARG | LYS | GLY |
Samples:
sample_1: hypothetical protein tm1012, [U-13C; U-15N], 1 mM; NaCl 500 mM; Tris 10 mM
cond_set1: ionic strength: 0.50 M; pH: 7.7; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| CBCAcoNH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNcoCA | not available | not available | not available |
| HBHAcoNH | not available | not available | not available |
| CCTOCSY NH | not available | not available | not available |
| HCCTOCSY NH | not available | not available | not available |
| 13C HSQC | not available | not available | not available |
| HCCHTOCSY | not available | not available | not available |
| HCCHCOSY | not available | not available | not available |
| CCHTOCSY | not available | not available | not available |
| 15N NOESY | not available | not available | not available |
| 13C NOESY | not available | not available | not available |
Software:
SPARKY v3.95 - assignemt, spectral analysis
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 800 MHz
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| GB | AAD36089 ACB10124 ACM23742 AGL49939 AHD19079 |
| REF | NP_228818 WP_004080525 YP_001739807 YP_002535108 YP_007977364 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks