BMRB Entry 7085
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR7085
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Wu, Bin; Yee, Adelina; Lemak, Alexander; Ramelot, Theresa; Semesi, Anthony; Kennedy, Michael; Arrowsmith, Cheryl. "Solution structure of hypothetical protein yst6499 from Saccharomyces
cerevisiae/ Northeast Structural Genomics Consortium Target YT727/ Ontario
Center for Structural Proteomics Target yst6499" The BMRB entry is the only known published source for the data..
Assembly members:
hypothetical protein yst6499, polymer, 120 residues, 13343.00 Da.
Natural source: Common Name: Saccharomyces cerevisiae Taxonomy ID: 2303 Superkingdom: Bacteria Kingdom: not available Genus/species: saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET15B
Entity Sequences (FASTA):
hypothetical protein yst6499: MGSSHHHHHHSSGRENLYFQ
GHMKSSIPITEVLPRAVGSL
TFDENYNLLDTSGVAKVIEK
SPIAEIIRKSNAELGRLGYS
VYEDAQYIGHAFKKAGHFIV
YFTPKNKNREGVVPPVGITN
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 405 |
| 15N chemical shifts | 91 |
| 1H chemical shifts | 679 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | yst6499 | 1 |
Entities:
Entity 1, yst6499 120 residues - 13343.00 Da.
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | |
| 2 | SER | SER | GLY | ARG | GLU | ASN | LEU | TYR | PHE | GLN | |
| 3 | GLY | HIS | MET | LYS | SER | SER | ILE | PRO | ILE | THR | |
| 4 | GLU | VAL | LEU | PRO | ARG | ALA | VAL | GLY | SER | LEU | |
| 5 | THR | PHE | ASP | GLU | ASN | TYR | ASN | LEU | LEU | ASP | |
| 6 | THR | SER | GLY | VAL | ALA | LYS | VAL | ILE | GLU | LYS | |
| 7 | SER | PRO | ILE | ALA | GLU | ILE | ILE | ARG | LYS | SER | |
| 8 | ASN | ALA | GLU | LEU | GLY | ARG | LEU | GLY | TYR | SER | |
| 9 | VAL | TYR | GLU | ASP | ALA | GLN | TYR | ILE | GLY | HIS | |
| 10 | ALA | PHE | LYS | LYS | ALA | GLY | HIS | PHE | ILE | VAL | |
| 11 | TYR | PHE | THR | PRO | LYS | ASN | LYS | ASN | ARG | GLU | |
| 12 | GLY | VAL | VAL | PRO | PRO | VAL | GLY | ILE | THR | ASN |
Samples:
sample_1: hypothetical protein yst6499, [U-13C; U-15N], 1 mM; NaCl 250 mM; tris 10 mM
cond_set1: ionic strength: 0.25 M; pH: 7.1; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N HSQC | not available | not available | not available |
| HNCO | not available | not available | not available |
| CBCAcoNH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNcoCA | not available | not available | not available |
| CCTOCSY_NH | not available | not available | not available |
| HCCTOCSY_NH | not available | not available | not available |
| 13C HSQC | not available | not available | not available |
| HCCHTOCSY | not available | not available | not available |
| HCCHCOSY | not available | not available | not available |
| CCHTOCSY | not available | not available | not available |
| 15N NOESY | not available | not available | not available |
| 13C NOESY | not available | not available | not available |
Software:
SPARKY v3.95 - assignemt, spectral analysis
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | GAA26128 |
| EMBL | CAY82229 |
| GB | AHY77122 EDN62841 EDV12232 EEU08283 EIW08126 |
| REF | NP_061494 |
| SP | Q3E841 |
| TPG | DAA10576 |
| AlphaFold | Q3E841 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks