BMRB Entry 7074
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PDB ID: 2gmg
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, LACS
BMRB Entry DOI: doi:10.13018/BMR7074
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Wang, Xu; Lee, Han-Seung; Sugar, Frank; Jenney, Francis; Adams, Michael; Prestegard, James. "PF0610, a novel winged helix-turn-helix variant possessing a rubredoxin-like
Zn ribbon motif from the hyperthermophilic archaeon, Pyrococcus furiosus" Biochemistry 46, 752-761 (2007).
PubMed: 17223696
Assembly members:
Pf0610, polymer, 105 residues, 12139 Da.
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Pyrococcus furiosus Taxonomy ID: 2261 Superkingdom: Archaea Kingdom: Euryarchaeota Genus/species: Pyrococcus furiosus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Pf0610: AHHHHHHGSATRREKIIELL
LEGDYSPSELARILDMRGKG
SKKVILEDLKVISKIAKREG
MVLLIKPAQCRKCGFVFKAE
INIPSRCPKCKSEWIEEPRF
KLERK
- assigned_chemical_shifts
- RDCs
| Data type | Count |
| 13C chemical shifts | 391 |
| 15N chemical shifts | 90 |
| 1H chemical shifts | 660 |
| residual dipolar couplings | 209 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Pf0610 | 1 |
| 2 | ZINC (II) ION | 2 |
Entities:
Entity 1, Pf0610 105 residues - 12139 Da.
The first 9 residues are part of the His tag. Pf0610 protein starts at residue 10
| 1 | ALA | HIS | HIS | HIS | HIS | HIS | HIS | GLY | SER | ALA | ||||
| 2 | THR | ARG | ARG | GLU | LYS | ILE | ILE | GLU | LEU | LEU | ||||
| 3 | LEU | GLU | GLY | ASP | TYR | SER | PRO | SER | GLU | LEU | ||||
| 4 | ALA | ARG | ILE | LEU | ASP | MET | ARG | GLY | LYS | GLY | ||||
| 5 | SER | LYS | LYS | VAL | ILE | LEU | GLU | ASP | LEU | LYS | ||||
| 6 | VAL | ILE | SER | LYS | ILE | ALA | LYS | ARG | GLU | GLY | ||||
| 7 | MET | VAL | LEU | LEU | ILE | LYS | PRO | ALA | GLN | CYS | ||||
| 8 | ARG | LYS | CYS | GLY | PHE | VAL | PHE | LYS | ALA | GLU | ||||
| 9 | ILE | ASN | ILE | PRO | SER | ARG | CYS | PRO | LYS | CYS | ||||
| 10 | LYS | SER | GLU | TRP | ILE | GLU | GLU | PRO | ARG | PHE | ||||
| 11 | LYS | LEU | GLU | ARG | LYS |
Entity 2, ZINC (II) ION - Zn - 65.409 Da.
| 1 | ZN |
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